Ganoderma lucidum Extract Modulates Gene Expression Profiles Associated with Antioxidant Defense, Cytoprotection, and Senescence in Human Dermal Fibroblasts: Investigation of Quantitative Gene Expression by qPCR

Current Issues in Molecular Biology, Год журнала: 2025, Номер 47(2), С. 130 - 130

Опубликована: Фев. 18, 2025

Cellular senescence plays a crucial role in skin aging, with senescent dermal fibroblasts contributing to reduced elasticity and increased inflammation. This study investigated the potential of Ganoderma lucidum (Reishi) ethanol extract modulate phenotype human fibroblasts. Reishi powder two different vendors was used. The produced by extracting for at least three weeks 40% room temperature. Etoposide-induced were treated extracts from commercial sources 14 days. Gene expression analysis performed using qPCR assess makers, antioxidant defense, extracellular matrix remodeling. Results showed that significantly upregulated cytoprotective genes, including Heme oxygenase 1 (HO-1), γ-Glutamylcysteine synthetase (γGCS-L), NAD(P)H dehydrogenase [quinone] (NQO1), compared untreated controls. Importantly, treatment suppressed p16INK4a, key marker cellular senescence, while transiently upregulating p21Cip1. also demonstrated senolytic properties, reducing percentage cells as measured senescence-associated β-galactosidase staining. However, did not mitigate upregulation MMP1 IL-8 one group, indicating differences preparations vendors. These findings suggest may help alleviate some aspects fibroblasts, primarily through enhanced defense cytoprotection, potentially offering novel approach combat aging.

Язык: Английский

---

Network Pharmacology and Experimental Validation Reveal Ganodermanontriol Modulates Pneumonia via TNF/NF‐κB/MAPKs Signaling Pathway

Food Science & Nutrition, Год журнала: 2025, Номер 13(4)

Опубликована: Март 25, 2025

Ganoderma lucidum (Leyss. ex Fr.) Karst, commonly known as Lingzhi, has long been employed in traditional Chinese medicine for its medicinal properties, particularly alleviating respiratory issues like cough and asthma. Recognized both a therapeutic agent an edible supplement, Lingzhi is celebrated health-promoting benefits. Despite widespread use, the effectiveness of G. treating pneumonia not extensively studied, highlighting need further research. This research aimed to evaluate potential treatment uncover mechanisms behind effects, specifically examining how active constituents influence inflammatory pathways. The study utilized approaches such network pharmacology, bioinformatics, molecular docking, vivo experiments. High-performance liquid chromatography (HPLC) chromatography-mass spectrometry (LC-MS) analyses revealed eight triterpenoids lucidum, with ganodermanontriol being most prominent. Molecular docking studies anticipated interactions between these compounds target proteins, while experiments on pneumonia-induced rat models assessed efficacy ganodermanontriol. Additionally, HPLC LC-MS confirmed presence ethanol extract predominantly Network pharmacology identified key genes-including TNF, EGFR, ESR1, HIF1A, HSP90AA1, SRC-that played significant roles regulation In results demonstrated that mitigated lung tissue damage rats experimentally induced by reducing release mediators. Further mechanistic showed downregulated TNF-α inhibited NF-κB/MAPKs signaling These findings suggested holds promising anti-inflammatory targeting TNF/NF-κB/MAPKs pathway, offering novel approach.

Язык: Английский

---

Rejuvenation of mesenchymal stem cells by human peripheral blood lymphocytes: A dual-mechanism for targeting senescent cell clearance and promoting cell proliferation in a coculture system

Опубликована: Апрель 3, 2025

Background: In the in-vitro expansion of mesenchymal stem cells (MSCs), replicative or stress-induced senescence poses a significant challenge, leading to loss their cellular properties and therapeutic functions. Currently, there is lack efficient strategies address this issue. Methods: Here we presented novel approach combat promote cell proliferation by coculturing human MSCs with peripheral blood lymphocytes (PBLs). After treatment PBL from tenth passage (P10), secretion profile PBLs was assessed RNA sequencing. RNA-seq iTRAQ quantitative proteomics-seq analyses were used determine transcriptomic proteomic changes, respectively. Results: heterogeneous population comprising both aged nonaged cells, effector rather than cytokines, exhibited dual role. They selectively induced apoptosis in facilitating p53 SUMOylation activating p53-dependent mitochondrial pathway, while simultaneously safeguarding younger against promoting Serpinb2/NF-κB signaling. This resulted decrease an enrichment rejuvenated MSCs. process effectively reversed phenotype, remodeling stemness characteristics enhancement functionality within MSC population.Furthermore, enhanced efficacy favorable safety profile. Conclusion: rejuvenate promptly removing enhancing proliferative capacity cells. work provides ideal method for obtaining substantial meeting quality requirements therapy.

Язык: Английский

---

Ganoderic acid a potentiates cisplatin’s cytotoxicity on gallbladder cancer cells by promoting DNA damage and inhibiting cell stemness

World Journal of Surgical Oncology, Год журнала: 2025, Номер 23(1)

Опубликована: Апрель 21, 2025

Ganoderma acid A (GAA), a triterpenoid compound from lucidum, has gained attention for its anti-tumor properties. Herein, we hypothesized that GAA may enhance cisplatin's (DDP) anticancer effect in gallbladder cancer (GBC) cells by promoting DNA damage response, particularly through upregulation of markers such as γH2AX, p-ATM, p-ATR, and p-p53, reducing cell stemness downregulating like SOX2, Oct4, NANOG. The human GBC line GBC-SD epithelial HGBEC were cultured RPMI-1640 DMEM/F12 media with 10% fetal bovine serum. Cells treated 2 µM DDP 60 24 h. To evaluate the toxicity normal cells, under same conditions. Cell viability was assessed CCK-8 assay, colony formation measured 6-well plates. Apoptosis evaluated TUNEL using comet assay. Stemness analyzed spheroid CD44 immunofluorescence staining. Western blot analysis performed to expression apoptotic, stemness, (Bax/Bcl-2, cleaved-caspase 3, NANOG, p-p53). results showed significantly reduced concentration-dependent manner (p < 0.05). combined treatment further decreased viability, IC50 value 8.98 4.07 Colony inhibited 0.05), apoptosis increased, assay revealed increased pro-apoptotic proteins Bax/Bcl-2 3(p p-p53 altered Specifically, p53 indicating enhanced response can effects on inhibiting supporting an adjuvant warrants validatory preclinical studies.

Язык: Английский

---

Natural compounds from traditional Chinese medicine regulating stem cell fate: identification and therapeutic potential

Chinese Herbal Medicines, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

---