Cellular Signalling, Год журнала: 2023, Номер 108, С. 110698 - 110698
Опубликована: Май 4, 2023
Язык: Английский
Life Sciences, Год журнала: 2023, Номер 340, С. 122370 - 122370
Опубликована: Дек. 22, 2023
Язык: Английский
Процитировано
11
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(9), С. 4905 - 4905
Опубликована: Апрель 30, 2024
Osteoarthritis is more prevalent than any other form of arthritis and characterized by the progressive mechanical deterioration joints. Glucosamine, an amino monosaccharide, has been used for over fifty years as a dietary supplement to alleviate osteoarthritis-related discomfort. Silibinin, extracted from milk thistle, modifies degree glycosylation target proteins, making it essential component in treatment various diseases. In this study, we aimed investigate functional roles glucosamine silibinin cartilage homeostasis using TC28a2 cell line. Western blots showed that suppressed N-glycosylation gp130, EGFR, N-cadherin proteins. Furthermore, both differentially decreased increased proteins such Snail, KLF4 cells. We observed compounds dose-dependently induced proliferation Our MitoSOX DCFH-DA dye data 1 µM mitochondrial reactive oxygen species (ROS) generation cytosol ROS generation, whereas JC-1 red aggregates, resulting increase red/green fluorescence intensity ratio, while all tested concentrations green monomers, decreases ratio. increasing subG1 S populations decreasing G1 G2/M with amounts glucosamine, led increases subG1, S, MTT cytotoxicity cells dose-dependent manner. Regarding endoplasmic reticulum stress, expression CHOP level p-eIF2α/eIF2α. With respect O-GlcNAcylation status, reduced levels O-GlcNAc transferase hypoxia-inducible factor alpha. examined mRNAs related these processes. summary, our findings demonstrated modulated cellular proliferation, membrane potential, cycle profile, autophagy. Therefore, conclude not only mediate modifications but also regulate processes human chondrocytes.
Язык: Английский
Процитировано
4
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(15), С. 8212 - 8212
Опубликована: Июль 27, 2024
Ferroptosis is a form of iron-dependent regulated cell death caused by the accumulation lipid peroxides. In this review, we summarize research on impact ferroptosis disease models and isolated cells in various types arthritis. While most studies have focused rheumatoid arthritis (RA) osteoarthritis (OA), there limited spondylarthritis crystal arthropathies. The effects inducing or inhibiting strongly depend studied type. search for new therapeutic targets, chondrocytes might promising any type On other hand, induction may also lead to desired decrease synovial fibroblasts RA. Thus, must consider cell-type-specific Further investigation needed clarify these complexities.
Язык: Английский
Процитировано
4
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(17), С. 9398 - 9398
Опубликована: Авг. 29, 2024
Articular cartilage receives nutrients and oxygen from the synovial fluid to maintain homeostasis. However, compared tissues with abundant blood flow, articular is exposed a hypoxic environment (i.e., physioxia) has an enhanced stress response. Hypoxia-inducible factors (HIFs) play pivotal role in this physioxic environment. In normoxic conditions, HIFs are downregulated, whereas they upregulated. The HIF-α family comprises three members: HIF-1α, HIF-2α, HIF-3α. Each member distinct function cartilage. osteoarthritis, which primarily caused by degeneration of cartilage, HIF-1α upregulated chondrocytes believed protect acting anabolically on it. Conversely, contrast HIF-2α exerts catabolic influence It may therefore be possible develop new treatment for OA controlling expression drugs or altering joints.
Язык: Английский
Процитировано
4
Cellular Signalling, Год журнала: 2023, Номер 108, С. 110698 - 110698
Опубликована: Май 4, 2023
Язык: Английский
Процитировано
9