Counteracting Skin Aging In Vitro by Phytochemicals

Journal of Cellular and Molecular Medicine, Год журнала: 2025, Номер 29(7)

Опубликована: Апрель 1, 2025

ABSTRACT The skin is the most extensive organ in human body. Photo exposure to ultraviolet (UV) rays causes several damages cells, including premature aging, onset of possible DNA mutations, and risk developing cancers, melanoma. Protecting from damaging effects sun through application creams filters important prevent irreversible damages. Several natural extracts biomolecules with antioxidant activity are widely used production dietary supplements or topical products, for prevention treatment affections. Within this context, we pre‐treated fibroblasts (HFF1), skin‐isolated stem cells (SSCs) keratinocytes (HaCaT) two containing a specific solar protection factor (SPF) 72 h then exposed UV light. Gene expression analysis was performed key cell cycle regulators (p16, p19, p21, p53 TERT). Cell senescence assessed by colorimetric assays beta‐galactosidase potential, revealing ability treated counteract free radical as result oxidative stress. Finally, mutations induced photo were studied. results obtained demonstrated that tested products elicit positive on all populations, preserving them senescence, being also able increase repairing mechanisms inducing youngest phenotype.

Язык: Английский

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Multifunctional Biomimetic Nanotherapeutics for Anti‐Oxidative and Anti‐Inflammatory Synergistic Therapy of Corneal Neovascularization

Aggregate, Год журнала: 2025, Номер unknown

Опубликована: Апрель 9, 2025

ABSTRACT Corneal neovascularization (CNV) is a debilitating ocular surface disease that severely compromises visual function and carries significant risk of vision loss. Despite its clinical impact, the development effective safe pharmacological treatments for CNV remains an unmet medical need. The pathogenesis largely driven by inflammation excessive oxidative stress. In this study, we introduce novel nanotherapeutic strategy utilizing vanadium carbide quantum dots (V 2 C QDs) with intrinsic nanozyme properties, co‐encapsulated plasmid encoding interleukin‐10 (IL‐10) within biomimetic metal‐organic framework (MOF) treatment CNV. To enhance targeting biocompatibility, nanoparticles (NPs) are further coated mesenchymal stem cell (MSC)‐derived membrane vesicles (CMVs), yielding final nanomedicine designated as MOF‐V C‐Plasmid@CMVs (MVPC). vitro studies demonstrate MVPC NPs effectively scavenge reactive oxygen species (ROS) induced tert ‐butyl hydroperoxide (tBOOH), mitigating Moreover, successful delivery expression IL‐10 in RAW264.7 cells result elevated secretion, showcasing robust anti‐inflammatory activity. CMV coating facilitates targeted delivery, enabling efficient accumulation region following topical administration via eye drops. vivo experiments model rats reveal nanotherapeutics significantly suppress without inducing adverse effects. Collectively, study provides proof concept multifunctional platform CNV, offering promising clinically translatable approach challenging disease.

Язык: Английский

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Inhibiting the Histone Demethylase Kdm4a Restrains Cardiac Fibrosis After Myocardial Infarction by Promoting Autophagy in Premature Senescent Fibroblasts

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

Abstract Premature senescent fibroblasts (PSFs) play an important role in regulating the fibrotic process after myocardial infarction (MI), but their effect on cardiac fibrosis remains unknown. Here, investigation is aimed to determine whether PSFs contribute and underlying mechanisms involved. It observed that premature senescence of strongly activated injured myocardium at 7 days MI identified Kdm4a located by analysis scRNA‐seq data immunostaining staining. Moreover, fibroblast specific gain‐ loss‐of‐function assays showed promoted interstitial fibrosis, contributing remodeling advanced stage MI, without influencing early rupture. ChIP‐seq ChIP‐PCR revealed deficiency autophagy reducing Trim44 expression through increased levels H3K9me3 modification promoter region. Furthermore, a coculture system overexpression accumulation secretion senescence‐associated secretory phenotype (SASP) factors, subsequently inducing which could be reversed interference. induces Trim44‐mediated then facilitates ultimately resulting remodeling, not affecting ventricular

Язык: Английский

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Aquaporin Channels in Skin Physiology and Aging Pathophysiology: Investigating Their Role in Skin Function and the Hallmarks of Aging

Biology, Год журнала: 2024, Номер 13(11), С. 862 - 862

Опубликована: Окт. 24, 2024

This study examines the critical role of aquaporins (AQPs) in skin physiology and aging pathophysiology. The plays a vital maintaining homeostasis by acting as protective barrier against external pathogens excessive water loss, while also contributing to appearance self-esteem individuals. Key physiological features, such elasticity repair capability, are essential for its proper function. However, with aging, these characteristics deteriorate, reducing skin's ability tolerate environmental stressors which contribute well internal processes, negatively affect function, immune response, overall well-being. AQPs, primarily known facilitating transport, significant normal functions, including hydration movement molecules like glycerol hydrogen peroxide, influence various cellular processes functions. In this context, we categorized aquaporin dysfunction into several hallmarks mitochondrial dysfunction, senescence, stem cell depletion, impaired macroautophagy, dysbiosis, inflamm-aging. Eight (AQP1, 3, 5, 7, 8, 9, 10, 11) expressed cells, regulating migration, proliferation, differentiation, response. Dysregulation or altered expression proteins can enhance related pathologies activating hallmarks. provides valuable insights potential targeting mitigate improve physiologic

Язык: Английский

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Needle-Free Injection of Metformin Ameliorates Skin Photoaging Through Inhibition of Ferroptosis and Oxidative Stress

Discovery Medicine, Год журнала: 2024, Номер 36(184), С. 1080 - 1080

Опубликована: Янв. 1, 2024

Background: Skin photoaging is a complex process of skin aging caused by continuous exposure to ultraviolet (UV) radiation through oxidative stress and other pathways, yet effective treatments are scarce. Metformin drug with both anti-senescence antioxidant functions; however, there fewer studies on photoaging. The study aimed investigate the role needle-free injection metformin in alleviating B (UVB) induced photoaging, explore mechanisms which alleviates fibroblast inhibiting ferroptosis stress. Methods: In our study, we initially performed bioinformatic analysis gene expression profile (GSE38308), RNA sequencing (RNA-Seq) found that associated ferroptosis. We investigated potential skin-protective mechanism utilizing UVB-induced rat model human fibroblasts (HSF) treated UVB. For in vitro experiments, cellular senescence was detected using SA-β-galactosidase staining p16 western blot. Ferroptosis were assessed via blot (glutathione Peroxidase 4 (GPX4) nuclear factor erythroid-2-related 2 (Nrf2)), reactive oxygen species (ROS) levels, transmission electron microscope, Lillie's staining, immunofluorescence staining. During vivo administered jet injectors injected into backs rats. effectiveness Masson Results: pathway closely bioinformatics analysis. HSF cells, treatment exhibits following effects: reduction blue-stained granules decrease p16, indicating senescence. Moreover, leads decreased ROS levels increased stress-related protein Nrf2, suggesting inhibition within cells. Additionally, results an elevation GPX4 expression, ferroptosis-associated mitochondrial damage, decline Needle-free could directly achieve therapeutic effects affecting cells dermis. effectively improved rats compared group, ameliorated stress, reduced Conclusions: Our data highlights novel improves has good potential.

Язык: Английский

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