Combinatorial transcriptomic and genetic dissection of insulin/IGF‐1 signaling‐regulated longevity in Caenorhabditis elegans DOI Creative Commons
Seokjin Ham, Sieun S. Kim, Sang‐Soon Park

и другие.

Aging Cell, Год журнала: 2024, Номер 23(7)

Опубликована: Март 26, 2024

Abstract Classical genetic analysis is invaluable for understanding the interactions underlying specific phenotypes, but requires laborious and subjective experiments to characterize polygenic quantitative traits. Contrarily, transcriptomic enables simultaneous objective identification of multiple genes whose expression changes are associated with phenotypes. Here, we conducted crucial longevity using datasets daf‐2 /insulin/IGF‐1 receptor mutant Caenorhabditis elegans . Our unraveled epistatic relationships at level, in addition verifying genetically established interactions. combinatorial also revealed conferred by mutations. In particular, demonstrated that extent lifespan caused various alleles transcription factor daf‐16 / FOXO matched their effects on mutants. We identified aging‐regulating signaling pathways subsets structural functional RNA elements altered different Lastly, elucidated cooperation between several regulators, based combination molecular analysis. These data suggest biological processes coordinately exert networks. Together our work demonstrates utility dissection identifying important physiological processes, including aging longevity.

Язык: Английский

L-Theanine Extends the Lifespan of Caenorhabditis elegans by Reducing the End Products of Advanced Glycosylation DOI Creative Commons

Zhihang Huang,

Haiming Jing, Yan Pan

и другие.

Foods, Год журнала: 2025, Номер 14(2), С. 221 - 221

Опубликована: Янв. 13, 2025

L-theanine, a non-protein amino acid naturally occurring in tea leaves, is recognized for its antioxidant, anti-inflammatory, and neuroprotective properties. Despite known benefits, the mechanisms by which L-theanine influences lifespan extension remain poorly understood. This study investigated effects of on Caenorhabditis elegans explored underlying mechanisms. Our findings indicate that significantly diminishes accumulation advanced glycation end products (AGEs), are biomarkers closely linked to aging age-related diseases. Through an AGE-level analysis, we observed when administered during early adulthood, notably extended under both normal high-glucose-induced stress conditions. enhanced typical conditions provided protective against stress. A further analysis demonstrated extends modulating DAF-2/DAF-16 insulin-like signaling pathway reducing (AGEs). In summary, this identified as potential anti-aging intervention AGE regulating pathways. These provide new insights developing strategies lay groundwork research benefits mammals. Future studies could explore molecular mechanisms, test mammalian models, assess long-term side effects.

Язык: Английский

Процитировано

0

Combinatorial transcriptomic and genetic dissection of insulin/IGF‐1 signaling‐regulated longevity in Caenorhabditis elegans DOI Creative Commons
Seokjin Ham, Sieun S. Kim, Sang‐Soon Park

и другие.

Aging Cell, Год журнала: 2024, Номер 23(7)

Опубликована: Март 26, 2024

Abstract Classical genetic analysis is invaluable for understanding the interactions underlying specific phenotypes, but requires laborious and subjective experiments to characterize polygenic quantitative traits. Contrarily, transcriptomic enables simultaneous objective identification of multiple genes whose expression changes are associated with phenotypes. Here, we conducted crucial longevity using datasets daf‐2 /insulin/IGF‐1 receptor mutant Caenorhabditis elegans . Our unraveled epistatic relationships at level, in addition verifying genetically established interactions. combinatorial also revealed conferred by mutations. In particular, demonstrated that extent lifespan caused various alleles transcription factor daf‐16 / FOXO matched their effects on mutants. We identified aging‐regulating signaling pathways subsets structural functional RNA elements altered different Lastly, elucidated cooperation between several regulators, based combination molecular analysis. These data suggest biological processes coordinately exert networks. Together our work demonstrates utility dissection identifying important physiological processes, including aging longevity.

Язык: Английский

Процитировано

2