Redefinition of the Mora Romagnola Pig Breed Herd Book Standard Based on DNA Markers Useful to Authenticate Its “Mono-Breed” Products: An Example of Sustainable Conservation of a Livestock Genetic Resource

Animals, Год журнала: 2021, Номер 11(2), С. 526 - 526

Опубликована: Фев. 18, 2021

Mora Romagnola is an autochthonous pig breed, raised in the north of Italy. Mono-breed pork products this breed are part important niche value chain that intrinsically linked to conservation local genetic resources can only survive due premium price these obtain on market. However, added attracts fraudsters unscrupulously sell mis-labelled products, causing consumer distrust that, turn, undermines strategy breed. To monitor and better characterise we phenotyped 826 pigs for three breed-specific traits. Then, genotyped almost all living sows boars registered Herd Book (n. = 357 animals) polymorphisms MC1R NR6A1 genes (affecting coat colour vertebral number, respectively). The results were used re-define descriptors Romagnala included information allowed genotypes at two genes. A few did not carry excluded from its Book. Finally, evaluated usefulness DNA markers authenticate meat against derived other 11 breeds wild boars. our knowledge, one first examples established a direct link between standard with possibility mono-breed using specific purpose combat frauds and, indirectly, support livestock resource.

Язык: Английский

---

Genome-wide detection for runs of homozygosity analysis in three pig breeds from Chinese Taihu Basin and Landrace pigs by SLAF-seq data

Journal of Integrative Agriculture, Год журнала: 2022, Номер 21(11), С. 3293 - 3301

Опубликована: Янв. 1, 2022

Erhualian (E), Meishan (MS) and Mi (MI) pigs are excellent indigenous pig breeds in Chinese Taihu Basin, which have made great contributions to the genetic improvement of commercial pigs. Investigation structure inbreeding level 3 is significance for sustainable breeding The length number runs homozygosity (ROH) as well frequency genomes covered by ROH can be used indicators evaluate origin population. In this study, characteristics E, MS, MI Landrace (L) were analyzed SLAF-seq data, coefficient was calculated according (FROH). addition, we identified candidate genes genomic regions associated with ROH. A total 10,568 detected 116 individuals 4 breeds. analysis showed that there significant differences between Basin L, E similar. results FROH MS highest (0.25±0.07), while lower than L. Compared other populations, a higher long (>5 Mb), indicating recent times. large related reproductive traits located high region ROH, these expected marker-assisted selection (MAS) programs. Our findings provide theoretical support conservation Basin.

Язык: Английский

---

Persistence of autozygosity in crossbreds between autochthonous and cosmopolitan breeds of swine: a simulation study

animal, Год журнала: 2024, Номер 18(2), С. 101070 - 101070

Опубликована: Янв. 22, 2024

Crossbreeding might be a valid strategy to valorize local pig breeds. should reduce homozygosity and, as consequence, yield hybrid vigor for fitness and production traits. This study aimed quantify the persistence of autozygosity in terminal crossbred pigs compared with purebreds turn, identify genomic regions where autozygosity's would not found. The was based on genotyping data from 20 European breeds three cosmopolitan used simulate offspring. consisted two steps. First, one hundred matings were simulated each pairwise combination 23 considered (for total 276 combinations), ignoring sex parent individuals order generate purebred leveraging all germplasm available. Second, few preselected terminal-maternal breed pairs mimic realistic crossbreeding system: (i) Mora Romagnola (boars) or Cinta Senese crossed Large White (sows) Landrace (sows); (ii) Duroc (sows). Runs estimate genome-wide (FROH). Observed FROH higher than crossbreds, although some combinations showed other combinations. Among purebreds, highest values observed Turopolje (0.50 0.46, respectively). ranged 0.04 0.16 crossbreds Alentejana × Iberian, respectively. Persistence found several segments harboring quantitative trait loci (QTLs) literature. enriched QTLs involved fatty acid metabolism associated performance simulation shows that persists most crosses. Results suggest is implemented when autochthonous obtain vigor.

Язык: Английский

---

Genome‐wide scan for runs of homozygosity in Asian wild boars and Anqing six‐end‐white pigs

Animal Genetics, Год журнала: 2022, Номер 53(6), С. 867 - 871

Опубликована: Сен. 9, 2022

Abstract Inbreeding and loss of genetic diversity are serious problems in local Chinese pig breeds. Runs homozygosity (ROHs) contiguous lengths homozygous genotypes that can provide information on inbreeding levels, mating schemes, selection pressure, events. The Anqing six‐end‐white (AQ) pig, an important autochthonous breed bred the Anhui province China, plays a key role industry. In recent decades, AQ pigs have become closed with small population size conservation farms, raising issue decline. this study, we used 10× resequencing to detect whole genome 24 six Asian wild boars (AWBs). We Plink software analyze distribution ROHs based data. obtained 935.04 Gb raw data from results more than 45 822 239 SNPs. Additionally, identified 28 702 ROHs. Compared AWB, had higher ROH numbers, longer rates, F values, which revealed artificial influenced level. A total 307 205 islands were AWBs respectively. genes mainly enriched immune biological processes. Our findings useful reference for developing breeding programs maintain germplasm resources pigs.

Язык: Английский

---

Identification of Homozygous Regions With Adverse Effects on the Five Economic Traits of Duroc Pigs

Frontiers in Veterinary Science, Год журнала: 2022, Номер 9

Опубликована: Апрель 28, 2022

Runs of homozygosity (ROH) are widely used to estimate genomic inbreeding, which is linked inbreeding depression on phenotypes. However, the adverse effects specific homozygous regions phenotypic characteristics rarely studied in livestock. In this study, 50 K SNP data 3,770 S21 Duroc (American origin) and 2,096 S22 (Canadian pigs were investigate harmful ROH five economic traits. The results showed that two lines had different numbers distributions unfavorable ROHs, may be related selection directions intensities between lines. A total 114 58 segments found with significant traits pigs, respectively. Serval pleiotropic ROHs detected reduce or multiple performances populations. Candidate genes these shared mainly growth, fertility, immunity, fat deposition. We also observed some genotypes cause opposite This study not only enhances our understanding phenotypes, but indicates information could incorporated into breeding programs control detrimental regions.

Язык: Английский

---

A meta-analysis of genetic and phenotypic diversity of European local pig breeds reveals genomic regions associated with breed differentiation for production traits

Genetics Selection Evolution, Год журнала: 2023, Номер 55(1)

Опубликована: Дек. 7, 2023

Intense selection of modern pig breeds has resulted in genetic improvement production traits while the performance local remained lower. As have been bred extensive systems, they adapted to specific environmental conditions, resulting a rich genotypic and phenotypic diversity. This study is based on European that genetically characterized using DNA-pool sequencing data phenotypically breed level phenotypes related stature, fatness, growth, reproductive traits. These were analyzed dedicated approach detect signatures linked order uncover potential candidate genes may underlie adaptation environments.Analysis revealed four main axes variation represented by Iberian three (i.e. Large White, Landrace, Duroc). In addition, clustered according their geographical origin, for example French Gascon Basque breeds, Italian Apulo Calabrese Casertana Spanish Iberian, Portuguese Alentejano breeds. Principal component analysis distinguished larger leaner with better growth from smaller fatter low efficiency. Linking phenotype identified 16 significant genomic regions associated 24 2 192 reproduction. Among them, several contained possible biological effects For example, strong associations found stature two containing, respectively, ANXA4 ANTXR1 genes, fatness region containing DNMT3A POMC HSD17B7 gene.In this we used detecting supported at identify different living environments systems.

Язык: Английский

---

Signatures of positive selection after the introduction of genomic selection in the Finnish Ayrshire population

Journal of Dairy Science, Год журнала: 2024, Номер 107(7), С. 4822 - 4832

Опубликована: Март 14, 2024

The Finnish Ayrshire (FAY) belongs to the Nordic Red breeds and is characterized by high milk yield, components, good fertility, functional conformation. FAY breeding program based on genomic selection. Despite benefits of selection values, autozygosity in genome may increase due selection, increased cause inbreeding depression selected traits. However, there lack studies concerning signatures after introduction.The aim this study was identify introduction Genomic data included 45,834 SNPs. genotyped animals were divided into 2 groups: born before (6,108 cows) (47,361 cows). We identified using 3 complementary methods: identification from runs homozygosity (ROH) islands one decay site-specific extended haplotype between populations at SNP sites (Rsb).In total, we 34 ROH chromosomes 1, 3, 6, 8, 12–15, 17, 19, 22, 26 (between 1980 2011) 30 1–3, 13–17, 25–26 2015 2020). additionally detected 22 ΔROH 2–3, 11, 13, 14, 16, 18, 20, 25–26. Finally, a total 31 Rsb regions 2, 25 identified.Based results, has favored certain alleles haplotypes related traits relevant program: production, growth, beef production traits, feed efficiency. Several genes these e.g., PLA2G4A, MECR, CHUK, COX15, RICTOR, SHISA9, SEMA4G overlapped or partially observed signature regions. association genotypes within their effects should be studied genetic undergoing monitored population.

Язык: Английский

---

Base Characteristics, Preservation Methods, and Assessment of the Genetic Diversity of Autochthonous Breeds of Cattle, Sheep and Pigs in Serbia: A Review

Animals, Год журнала: 2024, Номер 14(13), С. 1894 - 1894

Опубликована: Июнь 27, 2024

Preserving local autochthonous domestic animal populations and the products derived from them is a crucial aspect of managing human utilization biosphere. This management approach aims to ensure sustainable benefits for both present future generations. The diversity plays vital role in functionality sustainability food production system. It encompasses productive non-productive aspects, contributing significantly overall health, nutrition, security landscape by providing wide range animal-derived resources. Based on data contained Draft Program Rural Development, significant presence more than 44 breeds animals has been noted Serbia. In order enable preservation animals, competent Ministry Agriculture Republic Serbia has, through number projects, implemented models farms (in situ), as well provided technical assistance small that keep collections. also helps population procure conducts product quality research, provides opportunities integrate conservation programs tourism. Given molecular characterization key factor breeds, Serbia, DNA markers are used identification investigate belonging specific or strain. All mentioned activities led an immediate increase which especially true cattle (Busha), sheep (Sjenicka, Svrljiska, Vlach-vitohorn) pigs (Mangalitsa, Moravka, Resavka) discussed this paper. addition measures undertaken preserve genetic resources (AnGR), it necessary continue work primarily ex situ prevent loss their gene pools. However, regardless evident effort made we believe there still lot room further improvement. refers advanced technologies have not applied so far, mostly related genomic regions associated with economic traits, resistance diseases, adaptability emerging climate changes. way, capacity functional characteristics species will be improved.

Язык: Английский

---

Genetic characterisation of the Nero d’Aspromonte pig population in Southern Italy

Italian Journal of Animal Science, Год журнала: 2024, Номер 23(1), С. 1358 - 1372

Опубликована: Сен. 6, 2024

Язык: Английский

---

Ascertaining the genetic background of the Celtic‐Iberian pig strain: A signatures of selection approach

Journal of Animal Breeding and Genetics, Год журнала: 2023, Номер 141(1), С. 96 - 112

Опубликована: Окт. 9, 2023

Celtic-Iberian pig breeds were majority in Spain and Portugal until the first half of 20th century. In 1990s, they nearly extinct as a result introduction foreign improved breeds. Despite its historical importance, genetic background strain is poorly documented. this study, we have identified genomic regions that might contain signatures selection peculiar lineage. A total 153 DNA samples pigs (Spanish Gochu Asturcelta Portuguese Bísara breeds), Iberian Alentejano Cinta Senese pig, Korean local Cosmopolitan (Hampshire, Landrace Large White individuals) analysed. pairwise-comparison approach was applied: test populations five other reference populations. Three different statistics (XP-EHH, FST ΔDAF) computed on each comparison. Strict criteria used to identify sweeps order reduce noise brought by breeds' severe population bottlenecks. Within population, SNPs construct potential candidate areas under only considered if four ten two-by-two pairwise comparisons at least two three statistics. Genomic constructed within subsequently overlapped putatively unique strain. These finally for enrichment analyses. 39 regions, mainly located SSC5 SSC9 covering 3130.5 kb, could be representative ancient Enrichment analysis allowed seven genes (NOL12, LGALS1, PDXP, SH3BP1, GGA1, WIF1, LYPD6). Other studies reported WIF1 gene associated with ear size, one characteristic traits The function related reproduction, adaptation immunity traits, indirectly fitting rusticity non-improved traditionally exploited semi-extensive conditions.

Язык: Английский

---