Fusobacterium necrophorum and Fusobacterium varium are commensal members of the bovine reproductive microbiota and may colonize calf prenatally DOI Creative Commons

Justine Kilama,

Carl R Dahlen,

Mina Abbasi

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

ABSTRACT Fusobacterium necrophorum is an important pathogen associated with several infectious diseases in cattle. However, recent sequencing-based studies have indicated that F. positively pregnancy beef cows and the most abundant genus bull seminal microbiota, suggesting potential role of reproductive health fertility. Here, we performed a comprehensive screening to 1) determine whether (subspecies [FNN] funduliforme [FNF]), varium (FV) are part commensal members microbiota cattle; 2) explore these spp. colonized calf prenatally. For this, screened 11 different sample types including bovine ram semen, vaginal uterine swabs, fecal samples, as well samples from 180- 260-days old fetuses their respective dams using both quantitative PCR (514 samples) targeted culturing (499 samples). By qPCR, all were detected across types, FNF being highly prevalent semen (66.7%) maternal ruminal fluids (87.1%), which was confirmed by culturing. All identified (3.1%-9.4%) placental caruncles, fetal fluids, meconium (2.7% - 26.3%) qPCR not isolated culture method. Overall, our results suggest member healthy male FNF, FNN FV present vagino-uterine intestine

Язык: Английский

Fusobacterium necrophorum and Fusobacterium varium are commensal members of the bovine reproductive microbiota and may colonize calf prenatally DOI Creative Commons

Justine Kilama,

Carl R Dahlen,

Mina Abbasi

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

ABSTRACT Fusobacterium necrophorum is an important pathogen associated with several infectious diseases in cattle. However, recent sequencing-based studies have indicated that F. positively pregnancy beef cows and the most abundant genus bull seminal microbiota, suggesting potential role of reproductive health fertility. Here, we performed a comprehensive screening to 1) determine whether (subspecies [FNN] funduliforme [FNF]), varium (FV) are part commensal members microbiota cattle; 2) explore these spp. colonized calf prenatally. For this, screened 11 different sample types including bovine ram semen, vaginal uterine swabs, fecal samples, as well samples from 180- 260-days old fetuses their respective dams using both quantitative PCR (514 samples) targeted culturing (499 samples). By qPCR, all were detected across types, FNF being highly prevalent semen (66.7%) maternal ruminal fluids (87.1%), which was confirmed by culturing. All identified (3.1%-9.4%) placental caruncles, fetal fluids, meconium (2.7% - 26.3%) qPCR not isolated culture method. Overall, our results suggest member healthy male FNF, FNN FV present vagino-uterine intestine

Язык: Английский

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