Altered immune surveillance of B and T cells in patients with persistent residual lung abnormalities 12 months after severe COVID-19

Respiratory Research, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 18, 2025

Post-COVID-19 respiratory sequelae often involve lung damage, which is called residual abnormalities, and potentially lead to chronic issues. The adaptive immune response, involving T-cells B-cells, plays a critical role in pathogen control, inflammation, tissue repair. However, the link between dysregulation development of abnormalities remains unclear. 109 patients discharged with after COVID-19 were followed for 12 months divided as full recovery (FRG, n = 88) persistent (PLAG, 21). Cell profiling analysis was done using flow cytometry at 24 h not antigen-specific vitro stimulation. Plasma or supernatant levels IFN-g, IL-4, IL-10, IgM, IgG assessed, 10 (5 FRG, 5 PLAG) randomly selected detailed cell phenotyping functional peripheral blood mononuclear cells cytometry. Compared FRG group, PLAG exhibited an increase unswitched (p 0.0159) decreased double-negative activated B-cells 0.0317), systemic IL-10 lower, displayed reduced frequency total impaired spontaneous IgM 0.0357) 0.0079) release culture. Regarding T-cells, showed reduction effector memory CD4 + TEMRA following Notably, group also higher frequencies central Th2 (GATA3+) response activation than 0.0079). Patients post-critical exhibit B-cell function, increased activation. These imbalances may contribute ongoing dysfunction warrant further investigation potential mechanism abnormalities. Larger studies are necessary confirm these findings.

Язык: Английский

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Associated factors related to production of autoantibodies and dermo-epidermal separation in bullous pemphigoid

Archives of Dermatological Research, Год журнала: 2025, Номер 317(1)

Опубликована: Янв. 24, 2025

Язык: Английский

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Phase 2‐3 Trial: Prevention of the Progression to Moderate and Severe COVID‐19 in SARS‐CoV‐2‐Infected Non‐Hospitalized Adults With Inhaled siRNA‐Based MIR 19

Allergy, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

ABSTRACT Background COVID‐19 continues to be a major global health challenge. Inhaled siRNA‐based MIR 19 has been shown reduce the time clinical improvement in patients hospitalized with moderate COVID‐19. Methods We conducted an open‐label, randomized, controlled multicenter phase 2b‐3 trial (NCT05783206) evaluating safety and efficacy of inhaled siR‐7‐EM/KK‐46 (MIR 19) (5.55 mg/day) comparison standard care (control group) outpatients mild ( N = 492 for each group). The primary endpoint was proportion who developed or severe by 28th day randomization. Results Moderate course disease detected 14 (2.85%) 34 (6.91%) mg) therapy groups, respectively (the difference proportions −4.107% [95% CI: −7.28% −1.03%] p 0.002)). Adverse events (AE) were reported 77 (15.65%) from group, while group AEs registered 100 (20.33%) patients. No severe, treatment‐related observed group. Conclusions siR‐7‐EM/KK‐46, SARS‐CoV‐2‐specific RNAi‐based drug, well‐tolerated significantly decreased progression moderate/severe

Язык: Английский

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The Omics Landscape of Long COVID—A Comprehensive Systematic Review to Advance Biomarker, Target and Drug Discovery

Allergy, Год журнала: 2025, Номер unknown

Опубликована: Март 14, 2025

ABSTRACT An estimated 10% of coronavirus disease (COVID‐19) survivors suffer from persisting symptoms referred to as long COVID (LC), a condition for which approved treatment options are still lacking. This systematic review (PROSPERO: CRD42024499281) aimed explore the pathophysiological mechanisms underlying LC and potential treatable traits across symptom‐based phenotypes. We included studies with primary data, written in English, focusing on omics analyses human samples patients persistent at least 3 months. Our search PubMed Embase, conducted January 8, 2024, identified 642 studies, 29 met inclusion criteria after full‐text assessment. The risk bias was evaluated using Joanna Briggs Institute appraisal tool. synthesis including genomics, transcriptomics, proteomics, metabolomics, metagenomics, revealed common findings associated fatigue, cardiovascular, pulmonary, neurological, gastrointestinal Key mitochondrial dysfunction, dysregulated microRNAs pulmonary tissue impairment, blood–brain barrier disruption, coagulopathy, vascular microbiome disturbances, microbial‐derived metabolite production inflammation. Limitations include cross‐study heterogeneity variability sampling methods. emphasizes complexity need further longitudinal omics‐integrated advance development biomarkers targeted treatments.

Язык: Английский

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Introduction

Опубликована: Янв. 1, 2025

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Risk factors for complicated community-acquired pneumonia in Egyptian children: a hospital-based 6-month study

Egyptian Journal of Bronchology, Год журнала: 2025, Номер 19(1)

Опубликована: Апрель 12, 2025

Abstract Background Community-acquired pneumonia (CAP) remains among the primary cause of morbidity and mortality in children globally. Despite improved treatment guidelines, still there is a vast increase incidence complications linked to CAP. Identifying various risk factors predisposing development complicated community-acquired (CCAP) paves way verify determine new approaches for prevention, early diagnosis, effective management such complications. Aim To identify elucidate that predispose diagnosed with develop local pulmonary (complicated pneumonia) our Egyptian community. Methods One-hundred pediatric cases CAP were involved case–control study, where classified into 2 groups depending on absence (41 cases) or presence (59 Data demographics, medical history, physical examination, laboratory radiological findings collected analyzed. Logistic regression analysis was utilized predict enhancing population. Results Median age studied patients 3.5 years ranging between 0.5 13 years. Gender wise, 55 (55%) population males. Patients living urban areas 47 (47%) patients. High-grade fever reported 91% patients, 63% them received antipyretic form NSAID. Previous COVID-19 infection positive 29% while 26% had previous hospital admission pneumonia. below 5th percentile weight height represented 19%. showed following complication starting over 2.5 more likely complications, an odds ratio (OR) 95% confidence interval (95% CI) 11.875 (3.626–38.885) ( P = 0.000). Urban residence emerged as another critical factor, 61.0% being dwellers OR 4.269 (1.794–10.155) 0.001) respectively. Clinical history 5.868 (1.152–29.883) 0.033), NSAID use 2.822 (1.219–6.532) 0.015), past 2.888 (1.095–7.616) 0.028) prevalent cases. Additionally, CCAP often CI ) 4.713 (1.274–17.434) 0.020) anemia 3.420 (1.158–10.103) 0.041) thrombocytosis 4.889 (1.935–12.351) 0.023). Conclusion The study highlights including following: than years, areas, percentile, fever, NSAID, weaning breastfeeding, exposure environmental tobacco smoke (ETS), daycare center attendance, COVID-19, hemoglobin level equal less 8.9 g/dl, thrombocytosis. Early identification targeted interventions prevention these will lower children.

Язык: Английский

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Impact of age and prior COVID-19 on the response to influenza a components in the 2020–2021 Fluzone vaccine

Vaccine, Год журнала: 2025, Номер 56, С. 127171 - 127171

Опубликована: Май 1, 2025

Язык: Английский

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Decision Trees for the Analysis of Gene Expression Levels of COVID-19: An Association with Alzheimer’s Disease

BioMedInformatics, Год журнала: 2025, Номер 5(2), С. 26 - 26

Опубликована: Май 9, 2025

COVID-19 has caused millions of deaths around the world. The respiratory system is main target this disease, but it also been reported to attack central nervous system, creating a neuroinflammatory environment with release proinflammatory cytokines. There are several studies suggesting possible relationship between Alzheimer’s disease and COVID-19. Therefore, in study, machine learning microarray analysis was performed identify key genes that may be associated disease. dataset identified as GSE177477, containing 47 samples. A bioconductor oligo package RStudio (version 4.3.3) used process normalize data. Subsequently, one-way ANOVA obtain differentially expressed genes. We decision tree generation classify study 1856 Three trees were generated where three (DNAJC16, TREM1, UCP2) differentiated patients. best obtained an accuracy 72.34%, sensitivity 72.34% specificity 86.17%. involved processes like those such inflammation process, amyloid pathologies, related type 2 diabetes mellitus.

Язык: Английский

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Distinct immunity dynamics of natural killer cells in mild and moderate COVID-19 cases during the Omicron variant phase

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Май 12, 2025

The SARS-CoV-2 Omicron variant is associated with milder COVID-19 symptoms than previous strains. This study analyzed alterations in natural killer (NK) cell-associated immunity dynamics mild and moderate cases during the phase of pandemic. We conducted a retrospective observational cohort patients aged ≥16 confirmed infection who were hospitalized at Tottori University Hospital between January 2022 May 2022. A total 27 included analysis. Of these, 11 16 diagnosed COVID-19, respectively, based on Japanese clinical practice guideline. Peripheral blood NK cell subsets surface markers, including activating receptor NKG2D inhibitory TIGIT, as well serum levels 24 immunoregulatory such cytokines cytotoxic mediators, measured admission recovery. In addition, to explore immune patterns disease severity, differences markers soluble UL16-binding protein 2 (sULBP2) clinically most symptomatic time point hospitalization visualized using volcano plot Spearman's rank correlation analysis principal component (PCA). Patients exhibited expanded unconventional CD56dimCD16- cells elevated expression lower mediators (granzyme A, granzyme B, granulysin). contrast, exhaustion, characterized by upregulation along increased mediators. identified that significantly IL-6 sULBP2 levels. revealed IL-6, IFN-γ, Fas, CXCL8 correlated sULBP2. PCA distinct clusters severity. results highlight cases. Elevated levels, their correlations inflammatory reflects response These findings provide insight into caused improve our understanding its immunological features.

Язык: Английский

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Gastrointestinal Barrier Disruption in Post‐COVID Syndrome Fatigue Patients

Allergy, Год журнала: 2025, Номер unknown

Опубликована: Май 15, 2025

ABSTRACT Background Post‐COVID Syndrome (PCS) is the term for a condition with persistent symptoms in proportion of COVID‐19 patients after asymptomatic, mild, or severe disease courses. Numbers vary, but current estimate that approximately 10% develop PCS. The aim our study was to evaluate impact SARS‐CoV‐2 infection on gastrointestinal (GI) tract and associations development PCS fatigue, post‐exertional malaise (PEM), orthostatic dysregulation, autonomous and/or neurocognitive dysregulation. Methods By combining medical record data from prospective observational symptom analysis before, during, infection, we aimed identify potential risk factors predictive markers Additionally, analyzed blood, saliva, stool samples this well‐characterized patient cohort biologically validate findings. Results We identified significant between pre‐existing GI complaints Fatigue. showed higher LBP/sCD14 ratios, lower IL‐33 levels, IL‐6 levels compared control groups. Our results highlight critical role post‐viral Conclusion propose viral disrupts pathways related innate immune response barrier function, evidenced by intestinal low‐grade inflammation leakage. Monitoring crucial identifying clinical phenotypes Understanding interaction infections, responses, gut integrity could lead more effective diagnostic treatment strategies, ultimately reducing burden patients.

Язык: Английский

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