An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques DOI
Patrick Madden, Yanique Thomas, Robert V. Blair

и другие.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Апрель 8, 2022

Abstract The systemic nature of SARS-CoV-2 infection is highly recognized, but poorly characterized. A non-invasive and unbiased method needed to clarify whole body spatiotemporal dynamics after transmission. We recently developed a probe based on the anti-SARS-CoV-2 spike antibody CR3022 study pathogenesis in vivo. Herein, we describe its use immunoPET investigate three rhesus macaques. Using PET/CT imaging macaques at different times post-SARS-CoV-2 inoculation, track 64Cu-labelled CR3022-F(ab’)2 targeting protein within respiratory tract uncover novel sites infection. this method, uncovered differences lung pathology between with WA1 isolate delta variant, which were readily corroborated through computed tomography scans. 64Cu-CR3022-probe also demonstrated dynamic changes occurring 1- 2-weeks post-infection. Remarkably, robust signal was seen male genital (MGT) all animals studied. Infection MGT validated by immunofluorescence infected cells testicular penile tissue severe observed testes one animal results presented here underscore utility using understand pathogenicity discover new anatomical viral replication. provide direct evidence for revealing possible pathologic outcomes replication these sites.

Язык: Английский

Endothelial Dysfunction in COVID-19: A Unifying Mechanism and a Potential Therapeutic Target DOI Creative Commons
Pasquale Ambrosino, Ilenia Calcaterra,

Marco Mosella

и другие.

Biomedicines, Год журнала: 2022, Номер 10(4), С. 812 - 812

Опубликована: Март 30, 2022

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) generated a worldwide emergency, until the declaration of pandemic in March 2020. SARS-CoV-2 could be responsible for disease 2019 (COVID-19), which goes from flu-like illness to potentially fatal condition that needs intensive care. Furthermore, persistence functional disability and long-term cardiovascular sequelae COVID-19 survivors suggests convalescent patients may suffer post-acute syndrome, requiring care personalized rehabilitation. However, pathophysiology manifestations is still under study, as better comprehension these mechanisms would ensure more effective therapies. To date, mounting evidence crucial endothelial contribution clinical COVID-19, cells appear direct or indirect preferential target virus. Thus, dysregulation many homeostatic pathways endothelium has emerged hallmark severity COVID-19. aim this review summarize dysfunction with focus on pharmacological rehabilitation strategies targeting an attractive therapeutic option setting.

Язык: Английский

Процитировано

57
Preeclampsia and severe acute respiratory syndrome coronavirus 2 infection: a systematic review DOI Open Access
Giovanni Tossetta, Sonia Fantone, Nicola delli Muti

и другие.

Journal of Hypertension, Год журнала: 2022, Номер 40(9), С. 1629 - 1638

Опубликована: Июль 22, 2022

Objective: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of disease 2019 (COVID-19) that has rapidly spread worldwide, causing hundreds thousand deaths. Normal placentation characterized by many processes strictly regulated during pregnancy. If impaired, it can lead to gestational disorders, such as preeclampsia a multisystem disorder occurs in 2–8% pregnancies worldwide. Methods: We performed systematic search understand potential involvement SARS-CoV-2 onset using databases, PubMed and Web Science until 31 January 2022. Results: infection not only causes damage system but also infect human placenta cells impairing pivotal necessary for normal development. The inflammatory response trigged COVID-19 very similar one found suggesting possible link between Conclusion: Some studies showed affected had higher incidence compared with SARS-CoV-2-negative ones. However, increased blood pressure does allow associate hypertension common factor both conditions. At present, no diagnostic tools are available discriminate real from preeclampsia-like patients infection. Thus, new specific assure an appropriate diagnosis these patients, especially case severe disease.

Язык: Английский

Процитировано

46
Could SARS‐CoV‐2 infection affect male fertility and sexuality? DOI
Nicola delli Muti, Federica Finocchi, Giovanni Tossetta

и другие.

Apmis, Год журнала: 2022, Номер 130(5), С. 243 - 252

Опубликована: Фев. 3, 2022

Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is the cause of infectious disease (COVID‐19) and has rapidly spread worldwide, causing serious problems to healthcare systems many countries hundreds thousand deaths. In this review we discuss data from literature understand whether various districts male reproductive system may represent another vulnerable target for SARS‐CoV‐2. Studies were searched electronic databases such as Google Scholar, PubMed, Scopus, COVID‐19 specific LitCovid, until July 31, 2021. It appears that SARS‐CoV‐2 virus infection not only causes damage system, but could have a impact on patients modulating physiological processes. Like some other infections, also leads worsening semen quality an increase in oxidative stress (OS) levels. However, due limited number studies, it unclear deterioration parameters temporary or lasts over time. certainly important patients' function monitored after avoid health future.

Язык: Английский

Процитировано

42
Rapid assays of SARS-CoV-2 virus and noble biosensors by nanomaterials DOI Creative Commons
Yang Liu, Yilong Li,

Yuteng Hang

и другие.

Nano Convergence, Год журнала: 2024, Номер 11(1)

Опубликована: Янв. 8, 2024

Abstract The COVID-19 outbreak caused by SARS-CoV-2 in late 2019 has spread rapidly across the world to form a global epidemic of respiratory infectious diseases. Increased investigations on diagnostic tools are currently implemented assist rapid identification virus because mass and diagnosis might be best way prevent virus. This critical review discusses detection principles, fabrication techniques, applications with three categories: nuclear acid augmentation test, immunoassay test biosensors. Special efforts were put enhancement nanomaterials biosensors for rapid, sensitive, low-cost diagnostics Future developments suggested regarding potential candidates hospitals, clinics laboratories control prevention large-scale epidemic. Graphical

Язык: Английский

Процитировано

15
Effect of resveratrol on sperm motility in subjects affected by idiopathic asthenozoospermia: an in vitro study DOI
Nicola delli Muti, Alice Di Paolo, Gianmaria Salvio

и другие.

Tissue and Cell, Год журнала: 2025, Номер 95, С. 102857 - 102857

Опубликована: Март 16, 2025

Язык: Английский

Процитировано

1
ACE-2, TMPRSS2, and Neuropilin-1 Receptor Expression on Human Brain Astrocytes and Pericytes and SARS-CoV-2 Infection Kinetics DOI Open Access
Malik Johid Reza, Arpan Acharya, Sean N. Avedissian

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(10), С. 8622 - 8622

Опубликована: Май 11, 2023

Angiotensin Converting Enzyme 2 (ACE-2), Transmembrane Serine Protease (TMPRSS-2) and Neuropilin-1 cellular receptors support the entry of SARS-CoV-2 into susceptible human target cells are characterized at molecular level. Some evidence on expression mRNA protein levels in brain is available, but co-expression these confirmatory lacking. infects some cell types, infection susceptibility, multiple receptor density, kinetics rarely reported specific types. Highly sensitive Taqman ddPCR, flow-cytometry immunocytochemistry assays were used to quantitate ACE-2, TMPRSS-2 brain-extracted pericytes astrocytes, which an integral part Blood-Brain-Barrier (BBB). Astrocytes showed moderate ACE-2 (15.9 ± 1.3%, Mean SD, n = 2) (17.6%) positive cells, contrast show high (56.4 39.8%, 4) expression. Whereas variable (23.1 20.7%, 2), (30.3 7.5%, higher (667.2 232.3, 3) Co-expression astrocytes allows progression infection. roughly four-fold more virus culture supernatants than pericytes. "in vitro" viral may improve our understanding vivo". In addition, this study facilitate development novel strategies counter effects inhibit tissues prevent spread interference neuronal functions.

Язык: Английский

Процитировано

19
Cardiovascular Complications of Viral Respiratory Infections and COVID-19 DOI Creative Commons

Paweł Franczuk,

Michał Tkaczyszyn, Maria Kulak

и другие.

Biomedicines, Год журнала: 2022, Номер 11(1), С. 71 - 71

Опубликована: Дек. 27, 2022

Viral respiratory infections (VRI) are the most prevalent type of infectious diseases and constitute one common causes contact with medical care. Regarding pathophysiology cardiovascular system, VRI can not only exacerbate already existing chronic disease (such as coronary artery or heart failure) but also trigger new adverse events complications (e.g., venous thromboembolism), latter particularly in subjects multimorbidity disease-related immobilization. In current paper, we provide a narrative review diverse well summarize available data on pathology circulatory system course coronavirus 2019 (COVID-19).

Язык: Английский

Процитировано

24
Mechanisms of COVID-19 Associated Pulmonary Thrombosis: A Narrative Review DOI Creative Commons
Cristian‐Mihail Niculae, Adriana Hristea, Ruxandra Moroti

и другие.

Biomedicines, Год журнала: 2023, Номер 11(3), С. 929 - 929

Опубликована: Март 16, 2023

COVID-19, the infectious disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is frequently associated with pulmonary thrombotic events, especially in hospitalized patients. Severe SARS-CoV-2 infection characterized a proinflammatory state and an disbalance hemostasis. Immune pathology analysis supports inflammatory nature of arterial thrombi composed white blood cells, neutrophils, CD3+ CD20+ lymphocytes, fibrin, red platelets. cytokines, chemokines, complement system are key drivers immunothrombosis, as they induce damage endothelial cells initiate procoagulant positive feedback loops. Neutrophil extracellular traps induced COVID-19-associated “cytokine storm”, platelets, coagulation pathways close inflammation–endotheliopathy–thrombosis axis, contributing to SARS-CoV-2-associated events. The hypothesis immunothrombosis also supported minor role venous thromboembolism chest CT imaging data showing peripheral clots lesions high incidence events despite routine thromboprophylaxis. Understanding complex mechanisms behind COVID-19-induced thrombosis will lead future combination therapies for patients that would target crossroads pathways.

Язык: Английский

Процитировано

15
SARS-CoV-2, COVID-19, and Reproduction: Effects on Fertility, Pregnancy, and Neonatal Life DOI Creative Commons

Julien Harb,

Nour Debs,

Mohamad Rima

и другие.

Biomedicines, Год журнала: 2022, Номер 10(8), С. 1775 - 1775

Опубликована: Июль 22, 2022

Since its discovery in Wuhan, China, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread over the world, having a huge impact on people’s lives and health. The system is often targeted people with disease 2019 (COVID-19). virus can also infect many organs tissues body, including reproductive system. consequences of SARS-CoV-2 infection fertility pregnancy hosts are poorly documented. Available data other coronaviruses, such as (SARS-CoV) Middle Eastern Respiratory Syndrome (MERS-CoV) identified pregnant women vulnerable group increased pregnancy-related complications. COVID-19 was shown to pregnancy, which be seen either mother or fetus. Pregnant more likely require intensive care treatment than non-pregnant women, they susceptible giving birth prematurely their newborns admitted neonatal unit. Angiotensin converting enzyme (ACE2), key player ubiquitous renin-angiotensin (RAS), principal host cellular receptor for spike protein. ACE2 involved regulation both male female systems, suggesting that associated RAS dysfunction could affect reproduction. Herein, we review current knowledge about fertility, fetuses. Furthermore, describe effects vaccination

Язык: Английский

Процитировано

21
Correlation between Type I Interferon Associated Factors and COVID-19 Severity DOI Open Access

Dóra Bencze,

Tünde Fekete, Kitti Pázmándi

и другие.

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(18), С. 10968 - 10968

Опубликована: Сен. 19, 2022

Antiviral type I interferons (IFN) produced in the early phase of viral infections effectively inhibit replication, prevent virus-mediated tissue damages and promote innate adaptive immune responses that are all essential to successful elimination viruses. As professional IFN producing cells, plasmacytoid dendritic cells (pDC) have ability rapidly produce waste amounts IFNs. Therefore, their low frequency, dysfunction or decreased capacity IFNs might increase risk severe infections. In accordance with that, declined pDC numbers delayed inadequate could be observed patients coronavirus disease (COVID-19) caused by acute respiratory syndrome 2 (SARS-CoV-2), as compared individuals mild no symptoms. Thus, besides chronic diseases, those conditions, which negatively affect antiviral lengthen list factors for COVID-19. current review, we would like briefly discuss role dysregulation pDC/type axis COVID-19, introduce IFN-dependent factors, account an increased COVID-19 severity thus responsible different magnitude individual SARS-CoV-2.

Язык: Английский

Процитировано

18