Qing-Luo-Yin-induced SIRT1 inhibition contributes to the immune improvement of adjuvant-induced arthritis rats DOI
Dandan Wang,

Meng-Ke Song,

Qin Yin

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 17, 2024

Abstract The herbal formula Qing-Luo-Yin (QLY) was proved containing SIRT1 inhibitors. Whether they contribute to the anti-rheumatic effects is be confirmed. Adjuvant-induced arthritis (AIA) rats were treated by QLY or/and nicotinamide mononucleotide (NMN) for 38 days. After sacrifice, main tissues collected histological and western-blot experiments. Levels of rheumatoid (RA)-related indictors in blood or tissue homogenates detected commercial kits. Normal pre-adipocytes cultured relevant rat serums, medium monocytes culture. In replicate experiments, some received additional compounds silencing/overexpression treatments. Due spontaneous remission inflammation, didn’t further improve immune milieu AIA rats, but greatly eased paw edema joint injuries. Besides, it reversed triglyceride/glucose depletion liver adipose tissues, inhibited expression function SIRT1, causing concomitant changes related signals adipkines production. All weakened NMN, which activated increasing NAD serum from QLY-treated improved serum-induced metabolism secretion pre-adipocytes, reduced IL-1β, IL-6 TNF-α with corresponding medium. A mixture matrine, sinomenine, sophocarpine, dioscin, berberine showed similar on QLY-containing serum. eNAMPT decrease especially notable, obviously overexpression overshadowed SIRT1-silencing. inhibitors reshaped profiles tissues. It consequently mitigated eNAMPT-mediated inflammation rats.

Язык: Английский

QBT improved cognitive dysfunction in rats with vascular dementia by regulating the Nrf2/xCT/GPX4 and NLRP3/Caspase-1/GSDMD pathways to inhibit ferroptosis and pyroptosis of neurons DOI
Feng Lu, Yijin Wu,

Yan-Rong Yang

и другие.

International Immunopharmacology, Год журнала: 2024, Номер 142, С. 113070 - 113070

Опубликована: Сен. 12, 2024

Язык: Английский

Процитировано

5
GPIHBP1 increase accounts for rheumatic arthritis-related hypotriglyceridemia by facilitating lipids uptake of white adipose tissues DOI Creative Commons

Meng-Ke Song,

Meng-Fan Gu,

Lin Liu

и другие.

Arthritis Research & Therapy, Год журнала: 2025, Номер 27(1)

Опубликована: Янв. 25, 2025

Metabolism alteration is a common complication of rheumatic arthritis (RA). This work investigated the reason behind RA-caused triglyceride (TG) changes. Fresh RA patients' whole blood was transfused into NOD-SCID mice. Metabolism-regulatory tissues were examined after sacrifice. To verify findings, rats with long-lasting adjuvant-induced (AIA) analyzed. Some injected human plasma and GPIHBP1, their TG monitored. Various cells stimulated by cytokines or subjects' serum. pre-adipocytes cultured serum in presence HUVEC GPIHBP1. decrease occurred white adipose (WAT) blood-transfused mice chronic AIA rats. Fatty acids (FA) oxidation muscles accelerated bit, while catabolism status livers varied. TNF-α, IL-1β, IL-6 RA/AIA promoted expression utilization-related enzymes FA uptake transporters pre-adipocytes, but barely affected LPL. Mild stimulus GPIHBP1 release cells. increased change can rats, which overshadowed an injection excessive slightly inhibited LPL secretion pre-adipocytes. Both co-culture supplement reduced distribution on eliminated activity difference between normal serum-treated No observed these circumstances. RA-associated inflammation induces endothelial cells, facilitates hydrolysis to compensate loss WAT.

Язык: Английский

Процитировано

0
Diosgenin in Dioscorea spongiosa Suppresses Glycolysis-Driven Angiogenesis as a ROCK1 Inhibitor DOI

Kui Yang,

Xiaqing Zhou,

Kai Guo

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 19, 2025

The rhizome of Dioscorea spongiosa is used in Chinese herbal medicine as Bixie (BX), a drug with antirheumatic effects. To explore the mechanisms underlying its antiangiogenic actions, adjuvant-induced arthritis (AIA) rats were administered BX extract for 28 days. therapeutic efficacy was evaluated by serological, histological, and immunohistochemical tests. Metabolites quantified kits UPLC-MS. separated into 30 fractions silica gel chromatography. effects reagents assessed based on wound-healing, migration, tube-formation experiments using human umbilical vein endothelial cells (HUVECs). Protein targets pulled down biotin-conjugated compound. These proteins constituents BX-related samples identified LC-MS. target validated molecular simulation, immunoprecipitation, thermal shift assays, enzymatic digestion. mitigated metabolic abnormalities, inflammation, synovial invasion AIA rats. Angiogenesis white adipose tissues joints suppressed, shown decrease vessels, angiogenic cytokines, CD31. inhibited glycolysis capabilities HUVECs. expression some genes/proteins related to VEGF glycolytic signals reduced. Diosgenin only component high concentrations two most effective BX-treated rat's serum. It curbed glycolysis-fueled activation competed biotin-diosgenin when bound ROCK1. stabilized ROCK1 phosphorylation. Its VEGF-treated similar GSK429286 (a inhibitor) weakened arachidonate activator). hardly affected ROCK1-silenced In short, diosgenin inhibits suppresses angiogenesis via downregulation.

Язык: Английский

Процитировано

0
Qing-Luo-Yin Eased Adjuvant-Induced Arthritis by Inhibiting SIRT1-Controlled Visfatin Production in White Adipose Tissues DOI Creative Commons
Dandan Wang,

Meng-Ke Song,

Qin Yin

и другие.

Journal of Inflammation Research, Год журнала: 2024, Номер Volume 17, С. 6691 - 6706

Опубликована: Сен. 1, 2024

Nicotinamide adenine dinucleotide (NAD)-dependent deacetylase SIRT1 regulates both metabolism and immune functions. This study investigated if inhibitory property of herbal formula Qing-Luo-Yin (QLY) contributed to its anti-rheumatic effects.

Язык: Английский

Процитировано

2
PPARγ Functional Deficiency Expedited Fatty Acid Utilization in the Liver: A Foundation of Inflammatory Adipokine-Induced Hypolipemia in Rheumatoid Arthritis DOI
Yan Wang, Yunjun Ruan,

Lianjun He

и другие.

ACS Pharmacology & Translational Science, Год журнала: 2024, Номер 7(12), С. 3969 - 3983

Опубликована: Ноя. 29, 2024

Triglyceride (TG) and its derivatives tend to be decreased in rheumatoid arthritis (RA) patients' blood when inflammation progresses. Aside from the role as a lipid buffer, white adipose tissue (WAT) contributes this abnormality via adipokines, which regulate many metabolic signals. This work investigated adipokine-caused hepatic changes their involvement RA-related hypolipemia. Given immune similarities with RA pathological representativeness, adjuvant-induced (AIA) rats antigen-induced (AA) mice were adopted. Adipokine levels liver quantified, conditions assessed by oxidative/enzymatic indicators. Besides kit-based metabolite quantification, fatty acid accurately determined GC-MS. Metabolic differences between healthy AIA further characterized UPLC-MS

Язык: Английский

Процитировано

1
Qing-Luo-Yin-induced SIRT1 inhibition contributes to the immune improvement of adjuvant-induced arthritis rats DOI
Dandan Wang,

Meng-Ke Song,

Qin Yin

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 17, 2024

Abstract The herbal formula Qing-Luo-Yin (QLY) was proved containing SIRT1 inhibitors. Whether they contribute to the anti-rheumatic effects is be confirmed. Adjuvant-induced arthritis (AIA) rats were treated by QLY or/and nicotinamide mononucleotide (NMN) for 38 days. After sacrifice, main tissues collected histological and western-blot experiments. Levels of rheumatoid (RA)-related indictors in blood or tissue homogenates detected commercial kits. Normal pre-adipocytes cultured relevant rat serums, medium monocytes culture. In replicate experiments, some received additional compounds silencing/overexpression treatments. Due spontaneous remission inflammation, didn’t further improve immune milieu AIA rats, but greatly eased paw edema joint injuries. Besides, it reversed triglyceride/glucose depletion liver adipose tissues, inhibited expression function SIRT1, causing concomitant changes related signals adipkines production. All weakened NMN, which activated increasing NAD serum from QLY-treated improved serum-induced metabolism secretion pre-adipocytes, reduced IL-1β, IL-6 TNF-α with corresponding medium. A mixture matrine, sinomenine, sophocarpine, dioscin, berberine showed similar on QLY-containing serum. eNAMPT decrease especially notable, obviously overexpression overshadowed SIRT1-silencing. inhibitors reshaped profiles tissues. It consequently mitigated eNAMPT-mediated inflammation rats.

Язык: Английский

Процитировано

0