Laminaran potentiates cGAS-STING signaling to enhance antiviral responses
International Immunopharmacology,
Год журнала:
2025,
Номер
147, С. 114014 - 114014
Опубликована: Янв. 9, 2025
Язык: Английский
SESN1 negatively regulates STING1 to maintain innate immune homeostasis
Autophagy,
Год журнала:
2025,
Номер
unknown, С. 1 - 18
Опубликована: Фев. 13, 2025
STING1
is
a
central
hub
protein
of
CGAS-STING1
signaling
which
important
axis
to
sense
DNA
for
the
host
against
pathogens
infection
through
regulating
type
I
interferon
(IFN-I)
production.
However,
excessive
activation-induced
overproduced
IFN-I
triggers
tissue
damage
and
autoimmune
disorders.
Thus,
activity
must
be
precisely
regulated
immune
homeostasis.
Here,
we
discovered
SESN1
(sestrin
1)
as
an
essential
negative
regulator
maintain
Upon
herpes
simplex
virus-1
(HSV-1)
infection,
expression
was
downregulated,
enhanced
potentiality
virus
defense
host.
Consistently,
SESN1-deficient
mice
exhibited
stronger
ability
HSV-1
compared
wild-type
littermates.
Additionally,
found
decreased
in
systemic
lupus
erythematosus
(SLE)
patients
trex1
KO
mouse
model
disease.
Intriguingly,
replenishment
effectively
impressed
production
responses
PBMCs
human
SLE
specimens
both
vitro
vivo.
Mechanistically,
targeted
promoted
autophagic
degradation
by
facilitating
interaction
SQSTM1/p62
STING1.
Together,
our
study
uncovers
crucial
role
homeostasis
balance
anti-virus
autoimmunity
might
potential
therapeutic
target
infectious
diseases.Abbreviations:
BMDMs:
bone
marrow-derived
macrophages;
cGAMP:
cyclic
GMP-AMP;
CGAS:
GMP-AMP
synthase;
HTDNA:
herring
testes
DNA;
IFNA4:
alpha
4;
IFNB:
beta;
IRF3:
regulatory
factor
3;
ISD:
stimulatory
ISGs:
IFN-stimulated
genes;
PBMCs:
peripheral
blood
mononuclear
cells;
RSAD2:
radical
S-adenosyl
methionine
domain
containing
2;
SLE:
erythematosus;
STING1:
stimulator
response
cGAMP
interactor
1;
TBK1:
TANK
binding
kinase
1.
Язык: Английский
Characterization of microvessels in the human forehead dermis using intravascular dual perfusion and immunofluorescence staining
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 21, 2025
Skin
microcirculation
provides
essential
insights
in
clinical
practice.
However,
the
specific
characteristics
and
distribution
patterns
of
dermal
microarterioles
microvenules
remain
insufficiently
explored.
This
study
aimed
to
analyze
their
structural
differences
human
forehead
skin
using
an
innovative
intravascular
dual
perfusion
technique
combined
with
immunofluorescence
staining
distinguish
microvessel
types
within
dermis.
Using
two
post-mortem
cadaver
specimens,
lead
oxide-gelatin
was
applied
label
microarterioles,
latex
used
for
microvenules.
Tissue
sections
underwent
hematoxylin
eosin
staining,
cluster
differentiation
31
(CD31)
serving
as
a
general
vascular
marker
monocarboxylate
transporter
1
(MCT1)
venule-specific
marker.
The
analysis
revealed
significant
between
layers:
vessels
deep
dermis
had
larger
diameters
thicker
walls
than
those
superficial
layer,
while
density
higher
These
findings
demonstrate
distinct
layers,
reflecting
layer-specific
functional
demands.
Furthermore,
MCT1
identified
microvenules,
novel
method
combining
CD31
immunofluorescent
introduced
differentiate
from
results
offer
valuable
implications
surgical
planning,
grafting,
diagnostics
related
microcirculation.
Язык: Английский