International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(10), С. 4700 - 4700
Опубликована: Май 14, 2025
Plants
continuously
adapt
to
their
environments
by
responding
various
intrinsic
and
extrinsic
signals.
They
face
numerous
biotic
abiotic
stresses
such
as
extreme
temperatures,
drought,
or
pathogens,
requiring
complex
regulatory
mechanisms
control
gene
activity
proteome
for
survival.
Epigenetic
regulation
plays
a
crucial
role
in
these
adaptations,
potentially
leading
both
heritable
non-heritable
changes
across
generations.
This
process
enables
plants
adjust
expression
profiles
acclimate
effectively.
It
is
also
vital
plant
development
productivity,
affecting
growth,
yield,
seed
quality,
enabling
“remember”
environmental
stimuli
accordingly.
Key
epigenetic
that
play
significant
roles
include
DNA
methylation,
histone
modification,
ubiquitin
ligase
activity.
These
processes,
which
have
been
extensively
studied
the
last
two
decades,
led
better
understanding
of
underlying
expanded
potential
improving
agriculturally
economically
important
traits.
methylation
fundamental
regulates
altering
chromatin
structure.
The
addition
methyl
groups
cytosines
methylases
leads
suppression,
whereas
demethylases
reverse
this
effect.
Histone
modifications,
on
other
hand,
collectively
referred
“histone
code”,
influence
structure
promoting
either
transcription
silencing.
modifications
are
recognized,
added,
removed
variety
enzymes
act
practically
an
memory,
having
impact
responses
stimuli.
Finally,
complexes,
tag
specific
histones
proteins
with
ubiquitin,
regulation.
complexes
involved
protein
degradation
regulating
cellular
activities.
intricate
interplay
between
ligases
adds
complexity
our
expression,
generating
branching
network
interdependent
pathways.
A
deeper
helps
stressful
conditions
will
provide
valuable
insights
into
involved.
knowledge
could
pave
way
new
biotechnological
approaches
breeding
strategies
aimed
at
enhancing
crop
resilience,
sustainable
agriculture.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(7), С. 3053 - 3053
Опубликована: Март 26, 2025
During
drought,
a
major
abiotic
stressor
for
European
forests,
excessive
reactive
oxygen
species
(ROS)
are
produced,
causing
oxidative
damage
that
affects
structural
and
metabolic
tree
functions.
This
research
examines
the
effects
of
phosphorus
(P)
fertilization,
provenance
on
photosynthetic
pigments,
malondialdehyde
(MDA)
concentrations,
antioxidant
enzyme
activities
in
common
beech
(Fagus
sylvatica
L.)
sessile
oak
(Quercus
petraea
(Matt.)
Liebl.)
saplings
from
two
provenances.
In
garden
experiment,
four
treatments
were
applied:
regular
watering
with
(+PW)
without
P
fertilization
(-PW),
drought
(+PD)
(-PD)
fertilization.
Results
showed
increased
both
MDA
concentrations
activity,
particularly
superoxide
dismutase
(SOD),
catalase
(CAT),
peroxidase
(POD),
ascorbate
(APX),
which
responsible
ROS
scavenging.
Additionally,
chlorophyll
+
b
lower
drought-exposed
plants.
Phosphorus
minimally
affected
levels
but
enhanced
responses,
APX
CAT
during
drought.
Provenance
differences
notable,
drier
showing
better
adaptation,
reflected
higher
activities.
study
underscores
importance
defenses
coping
stress,
shaping
species'
adaptive
capacity.
Abscisic
acid
(ABA)
plays
a
central
role
in
regulating
plant
responses
to
abiotic
stress.
It
orchestrates
complex
regulatory
network
that
facilitates
the
transition
from
growth
defense.
Understanding
molecular
mechanisms
underlying
this
ABA-induced
defense
is
essential
for
elucidating
adaptive
strategies
under
environmental
stress
conditions.
In
study,
we
used
refined
dynamic
biomarker
(DNB)
approach
quantitatively
identify
critical
signal
(CTS)
and
characterize
regulated
stochasticity
during
Arabidopsis
thaliana.
By
integrating
high-resolution
time-series
RNA-seq
data
with
analysis,
identified
set
of
DNB
genes
serve
as
key
regulators
transition.
The
phase
was
precisely
at
ninth
time
point
(6
h
after
treatment),
which
marks
crucial
switch
growth-dominated
-oriented
state.
Gene
Ontology
(GO)
enrichment
analysis
revealed
significant
overrepresentation
defense-related
biological
processes,
while
STRING
strong
functional
interactions
between
differentially
expressed
(DEGs)
highlighted
hubs.
particular,
hub
such
PIF4,
TPS8,
NIA1,
HSP90-5
were
potential
master
ABA-mediated
activation,
highlighting
their
importance
adaptation.
network-driven
transcriptomic
study
provides
new
insights
into
basis
transitions
identification
CTS
perspective
on
conceptual
framework
improving
crop
resistance.
addition,
establishment
comprehensive
database
ABA-responsive
represents
valuable
resource
future
research
adaptation
resilience.
Life,
Год журнала:
2025,
Номер
15(5), С. 697 - 697
Опубликована: Апрель 25, 2025
Long
non-coding
RNAs
(lncRNAs)
have
been
defined
as
transcripts
exceeding
200
nucleotides,
which
play
essential
roles
during
transcriptional
and
post-transcriptional
regulation
in
multiple
biological
processes.
Understanding
R.
delavayi
lncRNAs
is
of
great
significance
it
profoundly
influenced
by
heat
stress.
In
this
research,
a
total
1145
differentially
expressed
(DE-lncRNAs)
9447
genes
(DEGs)
(log2|FC|
>
1,
p
<
0.05)
were
characterized
between
heat-stress-treated
groups
control
groups.
Further
analysis
showed
that
229
DE-lncRNAs
(144
upregulated
85
downregulated)
commonly
distributed
two
comparisons
(CK_vs._H3
CK_vs._H6).
We
further
investigated
the
cis-
trans-acting
DE-lncRNAs,
found
142
corresponded
to
1565
cis-acting
DEGs,
143
3417
DEGs.
KEGG
enrichment
these
target
revealed
DEGs
DE-lncRNA
primarily
enriched
five
twelve
pathways,
respectively.
Co-expression
network
common
pathways
57
co-expression
relationships
28
43
554
26
90
Six
six
their
used
candidate
verify
RNA-seq
data
using
qRT-PCR.
three
(TrxG,
PEPC,
CCR)
out
selected
for
subsequent
research.
This
study
examined
relationship
screened
can
potentially
provide
an
important
theoretical
basis
experimental
genetic
improvement
tolerance
delavayi.
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 28, 2025
AbstractBackground
DNA
methylation
plays
a
key
role
in
regulating
plant
growth
and
development,
secondary
metabolism,
environmental
adaptability
is
affected
by
methyltransferase
(C5-MTase)
demethylase
(dMTase).
However,
the
response
of
C5-MTasedMTase
genes
to
abiotic
stress
Isodon
rubescens
remains
largely
unknown.
Results
In
this
study,
eight
IrC5-MTase
five
IrdMTase
were
identified
within
genome
I.
rubescens.
The
IrC5-MTases
phylogenetically
divided
into
four
subfamilies
(CMT,
DRM,
MET
DNMT2),
two
subgroups
(ROS
DML)
on
basis
sequence
similarity,
conserved
motif
domain
architecture.
IrC5-MTase
IrdMTase
unevenly
distributed
5
4
chromosomes,
respectively.
Cis‑acting
element
analysis
revealed
that
enriched
with
multiple
light-,
phytohormone-
stress-responsive
elements.
Importantly,
level
was
influenced
different
5-azacytidine
(5-azaC)
concentrations
or
light
intensities
but
unaffected
NaCl
concentrations.
Accordingly,
expression
significantly
upregulated
downregulated
5-azaC
amounts
stress,
however,
no
conspicuous
differences
observed
under
degrees
stress.
Expression
correlation
IrCMT2a,
IrMET1,
IrDML4
most
closely
related
Conclusions
Our
results
reveal
characteristics
differential
5-azaC,
stresses
will
facilitate
future
epigenetic
studies
