Chitin Synthase Is Required for Cuticle Formation and Molting in the Chinese Mitten Crab Eriocheir sinensis

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(5), С. 2358 - 2358

Опубликована: Март 6, 2025

Chitin synthase is an essential enzyme of the chitin synthesis pathway during molting. In this study, we identified and characterized a (EsCHS) gene in Chinese mitten crab, Eriocheir sinensis. The spatio-temporal expression functional role EsCHS were investigated. open reading frame was 4725 bp long encoded 1574 amino acid residues that contained typical domain structure glycosyltransferase family 2. Phylogenetic analysis revealed belongs to group I family. found regenerative limbs, cuticle intestines. During molting cycle, began increase pre-molt stage reached significant peak post-molt stage. knockdown resulted downregulation biosynthesis genes, including TRE, HK, G6PI, PAGM UAP. Moreover, long-term RNAi thinning procuticles, abnormal high mortality, suggesting indispensable for formation conclusion, involved plays important E. These findings highlight potential incorporating into selective breeding programs optimize regulation improve growth performance crustacean aquaculture.

Язык: Английский

---

Key Neuropeptides Regulating Molting in Pacific White Shrimp (Penaeus vannamei): Insights from Transcriptomic Analysis

Animals, Год журнала: 2025, Номер 15(4), С. 540 - 540

Опубликована: Фев. 13, 2025

Molting is a vital physiological process essential for the growth and development of Penaeus vannamei, with significant implications aquaculture productivity. This study aimed to identify neuropeptide-related genes involved in molting through transcriptomic analysis. RNA sequencing pre-molt post-molt samples revealed 1203 differentially expressed (DEGs). Functional enrichment analysis indicated that these play roles cuticle formation regulation. Among DEGs, 243 were predicted be neuropeptides based on presence signal peptides absence transmembrane domains. Five key neuropeptide genes-PvCHH, PvMIH, PvEH I, PvCDA II-were identified as critical regulators molting. Their role was further validated RT-qPCR analysis, confirming their close association process. These highlighted this pivotal factors driving P. vannamei. The research are anticipated offer valuable insights into regulation Additionally, synthetic products hold promise improving consistency shrimp aquaculture.

Язык: Английский

---

ame-miR-5119-Eth axis modulates larval-pupal transition of western honeybee worker

Frontiers in Physiology, Год журнала: 2024, Номер 15

Опубликована: Сен. 27, 2024

The miRNA plays a key role in the regulation of hormone signaling insects. pathways by which miRNAs affect levels are unclear honeybee ( Apis mellifera ), an indispensable pollinator nature. In this study, ame-miR-5119 was overexpressed and knocked down larvae feeding mimics inhibitors, respectively, we determined that regulates through target gene ecdysis triggering Eth affects larval-pupal transition workers. results showed with length 19 nt targets six genes related to pathway. We focused on found exhibited reverse expression patterns during from larval pupal stages Dual luciferase assay confirmed negative regulatory between . Overexpression decreased mRNA level , receptor Ethr ) not significantly affected, but juvenile (JH) pathway acid methyltransferase Jhamt Krüppel homolog 1 Kr-h 1) were reduced. contrast, knockdown increased upregulated. did body weight. number overexpressing survived prepupal stage lower than control group, pupations reduced at 11-day-old. higher These indicated negatively indirectly inhibits Kr - h 1, JH biosynthesis, thereby preventing metamorphic larva pupa worker bees. findings provide evidence honey

Язык: Английский

---

Moult cycle and setal development of the Atlantic ditch shrimp Palaemon varians Leach, 1814

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 23, 2024

ABSTRACT The Atlantic ditch shrimp Palaemon varians Leach, 1814 is a common estuarine and brackish water species on Northern coasts. an appealing model organism for studying arthropod developmental processes, such as moulting (ecdysis). Detailed morphological information its moult cycle still lacking, hence we have characterised the changes in setal features corresponding to stages of P. grown under laboratory conditions. were differentiated described using microscopic analysis setae uropods based Drach’s classification system. Moult defined early late post-moult (A B), inter-moult (C), early-, mid- pre-moult (D 0 , D 1, 2 ), well ecdysis stage (E), actual shedding exuvia. Average duration was 8.7 days, where accounted longest 4.4 days average. This study provides reference determining without use invasive techniques, thus it suited repetitive observations individual track entire process.

Язык: Английский

---

Chitin Deacetylase 1 Gene as an Optimal RNAi-Based Target for Controlling the Tomato Leaf Miner Tuta absoluta

Insects, Год журнала: 2024, Номер 15(11), С. 838 - 838

Опубликована: Окт. 25, 2024

Chitin is a critical component of both the exoskeleton and internal structures insects, which can protect insects from mechanical damage, dehydration pathogen infection, plays significant role in molting process. deacetylases (CDAs), key enzymes involved chitin metabolism, are widely distributed among arthropods microorganisms. In this study, we identified

Язык: Английский

---

Moult cycle and setal development of the Atlantic ditch shrimp Palaemon varians Leach, 1814 (Decapoda: Caridea: Palaemonidae)

Journal of Crustacean Biology, Год журнала: 2024, Номер 44(4)

Опубликована: Ноя. 25, 2024

Abstract The Atlantic ditch shrimp Palaemon varians Leach, 1814 is a common estuarine and brackish water species on Northern coasts. an appealing model organism for studying arthropod developmental processes, such as moulting (ecdysis). Detailed morphological information its moult cycle still lacking, hence we have characterised the changes in setal features corresponding to stages of P. grown under laboratory conditions. were differentiated described using microscopic analysis setae uropods based Drach’s classification system. Moult defined early late post-moult (A B), inter-moult (C), early-, mid- pre-moult (D0, D1, D2), well ecdysis stage (E), actual shedding exuvia. Average duration was 8.7 days, where accounted longest 4.4 days average. This study provides reference determining without use invasive techniques, thus it suited repetitive observations individual track entire process.

Язык: Английский

---

Experimental approaches to hormones and behavior: Invertebrates

Elsevier eBooks, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Язык: Английский

---