Experimental Reporting of Fish Transcriptomic Responses in Environmental Toxicology and Ecotoxicology

Environmental Toxicology and Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Янв. 6, 2025

Due to its increasing affordability and efforts understand transcriptional responses of organisms biotic abiotic stimuli, transcriptomics has become an important tool with significant impact on toxicological investigations hazard risk assessments, especially during development application new approach methodologies (NAMs). Data generated using transcriptomic have directly informed adverse outcome pathway frameworks, chemical biological read across, aided in the identification points departure. Using data reporting frameworks for offers improved transparency reproducibility research opportunity identify barriers adoption these NAMs, environmental toxicology ecotoxicology aquatic models. Improved also allows reexamination existing data, limiting needs experiment replication further reducing animal experimentation. Here, we use a standardized form omics-based studies, Organisation Economic Co-operation Development omics framework, which specifically reports list parameters that should be included studies used regulatory context. We focused fish RNA- Sequencing (Seq)/microarray technologies within Inconsistencies among experimental designs (toxicology vs. molecular characterization) were observed addition foundational differences sample concentration or preparation quality can affect confidence results, contribute substantially understanding mechanisms toxicants toxins. Our findings present reporting. provide several recommendations as logical steps reduce ecotoxicology.

Язык: Английский

Evidence of the impacts of pharmaceuticals on aquatic animal behaviour (EIPAAB): a systematic map and open access database

Environmental Evidence, Год журнала: 2025, Номер 14(1)

Опубликована: Март 20, 2025

Abstract Background Over the last decade, pharmaceutical pollution in aquatic ecosystems has emerged as a pressing environmental issue. Recent years have also seen surge scientific interest use of behavioural endpoints chemical risk assessment and regulatory activities, underscoring their importance for fitness survival. In this respect, data on how pharmaceuticals alter behaviour animals appears to grown rapidly. Despite this, there been notable absence systematic efforts consolidate summarise field study. To address our objectives were twofold: (1) systematically identify, catalogue, synthesise primary research articles effects animal behaviour; (2) organise information into comprehensive open-access database scientists, policymakers, managers. Methods We searched two electronic databases (Web Science Scopus) supplemented these with additional article sources. The search string followed Population–Exposure–Comparison–Outcome framework capture that used an organism (population) test (exposure) (outcome). Articles screened stages: title abstract, by full-text screening alongside extraction. Decision trees designed priori appraise eligibility at both stages. Information study validity was collected but not basis inclusion. Data synthesis focused species, compounds, behaviour, quality themes enhanced sources metadata from online (e.g. National Center Biotechnology (NCBI) Taxonomy, PubChem, IUCN Red List Threatened Species). Review findings 5,988 articles, which 901 included final database, representing 1,739 unique species-by-compound combinations. includes over 48 (1974–2022), most having focus (510) fewer relating medical basic topics (233 158, respectively). 173 species (8 phyla 21 classes). Ray-finned fishes far common clade (75% evidence base), studies freshwater compared marine (80.4% versus 19.6%). 426 compounds; groups antidepressants (28%), antiepileptics (11%), anxiolytics (10%). Evidence impacts locomotion boldness/anxiety behaviours commonly assessed. Almost all scored laboratory setting, only 0.5% measured under conditions. Generally, we detected poor reporting and/or compliance several criteria. Conclusions Our map revealed rapid increase area past 15 years. highlight multiple areas now suitable quantitative where is lacking. some pitfalls method practice. More detailed would facilitate toxicology studies, assessment, management improve replicability. EIPAAB can be tool closing knowledge methodological gaps future.

Язык: Английский

Pharmaceutical pollution influences river-to-sea migration in Atlantic salmon ( Salmo salar )

Science, Год журнала: 2025, Номер 388(6743), С. 217 - 222

Опубликована: Апрель 10, 2025

Despite the growing threat of pharmaceutical pollution, we lack an understanding whether and how such pollutants influence animal behavior in wild. Using laboratory- field-based experiments across multiple years Atlantic salmon ( Salmo salar ; n = 730), show that globally detected anxiolytic pollutant clobazam accumulates brain exposed fish influences river-to-sea migration success. Clobazam exposure increased speed with which passed through two hydropower dams along their route, resulting more clobazam-exposed reaching sea compared controls. We argue effects may arise from altered shoaling to clobazam. Drug-induced behavioral changes are expected have wide-ranging consequences for ecology evolution wild populations.

Язык: Английский