Study on the Role and Mechanism of SLC3A2 in Tumor-Associated Macrophage Polarization and Bladder Cancer Cells Growth

Technology in Cancer Research & Treatment, Год журнала: 2024, Номер 23

Опубликована: Янв. 1, 2024

Background: Solute carrier family 3 member 2 (SLC3A2) is highly expressed in various types of cancers, including bladder cancer (BLCA). However, the role and mechanism SLC3A2 onset progression BLCA are still unclear. Methods: The interfering plasmid for was constructed transfected into cells. Cell proliferation, invasion, migration abilities were assessed to evaluate impact silencing on cell growth. M1 M2 macrophage polarization markers detected polarization. levels reactive oxygen species (ROS), lipid peroxidation, Fe 2+ , as well expression ferroptosis-related proteins, measured assess occurrence ferroptosis. Ferroptosis inhibitors used verify mechanism. Results: experimental results showed that lines. cells reduced after with SLC3A2. Interference led increase decreased M0 macrophages co-cultured tumor Additionally, interference increased ROS, downregulated solute 7 member11 (SLC7A11) glutathione peroxidase 4 (GPX4), while upregulated acyl-coA synthetase long chain (ACSL4) transferrin receptor 1 (TFR1) proliferation impeded by ferroptosis inhibitors. Conclusion: inhibited growth tumor-associated promoting

Язык: Английский

---

Integrative analysis of anoikis-related genes prognostic signature with immunotherapy and identification of CDKN3 as a key oncogene in lung adenocarcinoma

International Immunopharmacology, Год журнала: 2024, Номер 143, С. 113282 - 113282

Опубликована: Окт. 8, 2024

Язык: Английский

---

Gut microbiota influence on lung cancer risk through blood metabolite mediation: from a comprehensive Mendelian randomization analysis and genetic analysis

Frontiers in Nutrition, Год журнала: 2024, Номер 11

Опубликована: Сен. 11, 2024

Gut microbiota (GM) and metabolic alterations play pivotal roles in lung cancer (LC) development host genetic variations are known to contribute LC susceptibility by modulating the GM. However, causal links among GM, metabolite, genes, remain be fully delineated.

Язык: Английский

---

Identification of key genes and biological pathways in chronic obstructive pulmonary disease using bioinformatics and next generation sequencing data analysis

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 14, 2024

Abstract Identification of accurate biomarkers is still particularly urgent for improving the poor survival chronic obstructive pulmonary disease (COPD) patients. In this investigation, we aimed to identity potential in COPD via bioinformatics and next generation sequencing (NGS) data analysis. differentially expressed genes (DEGs) were identified using NGS dataset (GSE239897) from Gene Expression Omnibus (GEO) database. Subsequently, gene ontology (GO) pathway enrichment analysis was conducted evaluate underlying molecular mechanisms involved progression COPD. Protein-protein interaction (PPI), modules, miRNA-hub regulatory network TF-hub performed determine hub genes, miRNAs TFs. The receiver operating characteristic (ROC) diagnostic value genes. A total 956 overlapping DEGs (478 up regulated 478 down genes) dataset. mainly associated with GO functional terms pathways cellular response stimulus. stimulus, immune system neutrophil degranulation. There 10 (MYC, LMNA, VCAM1, MAPK6, DDX3X, SHMT2, PHGDH, S100A9, FKBP5 RPS6KA2) by PPI, conclusion, DEGs, relative terms, present investigation might aid understanding provide targets

Язык: Английский

---

Identification of a novel FOXO3 agonist that protects against alcohol induced liver injury

Biochemical and Biophysical Research Communications, Год журнала: 2024, Номер 704, С. 149690 - 149690

Опубликована: Фев. 17, 2024

Язык: Английский

---