Secretomes From Non‐Small Cell Lung Cancer Cells Induce Endothelial Plasticity Through a Partial Endothelial‐to‐Mesenchymal Transition
Cancer Medicine,
Год журнала:
2025,
Номер
14(5)
Опубликована: Март 1, 2025
The
tumor
microenvironment
(TME)
of
non-small
cell
lung
cancer
(NSCLC)
is
highly
heterogeneous
and
involved
in
tumorigenesis
resistance
to
therapy.
Among
the
cells
TME,
endothelial
are
associated
with
latter
processes
through
endothelial-to-mesenchymal
transition
(EndMT).
During
EndMT,
(ECs)
progressively
lose
their
phenotype
favor
a
mesenchymal
phenotype,
which
favors
production
cancer-associated
fibroblasts
(CAFs).
Our
study
aimed
investigate
consequences
exposure
different
secretomes
on
EC
plasticity.
Conditioned
media
(CM)
were
prepared
from
lines
A549,
H1755,
H23,
H1437,
H1975.
Proliferation
migration
ECs
treated
these
CMs
assessed
by
Cyquant
Incucyte
technologies,
respectively.
angiogenic
capacity
was
following
tubulogenesis
Matrigel.
Phenotypic
changes
detected
flow
cytometry.
Morphological
analysis
actin
fibers
performed
immunohistochemistry,
while
proteomic
mass
spectrometry
used
identify
protein
content
secretomes.
A
change
found
when
human
umbilical
vein
(HUVECs)
CMs.
This
phenotypic
morphological
change,
an
increase
both
stress
fiber
expression
spontaneous
migration.
Furthermore,
markers
(α-SMA
CD44)
confirmed
changes.
However,
did
not
modify
rate
double-labeled
(vWF+/α-SMA+
or
CD31+/CD44+).
Proteomic
identified
potential
targets
EndMT
therapeutic
relevance.
Taken
together,
data
suggest
that
can
induce
partial
EndMT.
Язык: Английский
CD40 is expressed in the subsets of endothelial cells undergoing partial endothelial–mesenchymal transition in tumor microenvironment
Cancer Science,
Год журнала:
2023,
Номер
115(2), С. 490 - 506
Опубликована: Дек. 18, 2023
Abstract
Tumor
progression
and
metastasis
are
regulated
by
endothelial
cells
undergoing
endothelial–mesenchymal
transition
(EndoMT),
a
cellular
differentiation
process
in
which
lose
their
properties
differentiate
into
mesenchymal
cells.
The
EndoMT
through
spectrum
of
intermediate
phases,
suggesting
that
some
remain
partial
state
exhibit
an
endothelial/mesenchymal
phenotype.
However,
detailed
analysis
has
been
hampered
the
lack
specific
markers.
Transforming
growth
factor‐β
(TGF‐β)
plays
central
role
induction
EndoMT.
Here,
we
showed
inhibition
TGF‐β
signaling
suppressed
human
oral
cancer
cell
xenograft
mouse
model.
By
using
genetic
labeling
lineage,
also
established
novel
reporter
system,
(EMRECs),
allow
visualization
sequential
changes
during
TGF‐β‐induced
Using
EMRECs,
characterized
gene
profiles
multiple
stages
identified
CD40
as
EndoMT‐specific
marker.
expression
was
upregulated
EndoMT,
but
decreased
full
Furthermore,
single‐cell
RNA
sequencing
tumors
revealed
enriched
population
expressing
both
Moreover,
EMRECs
enhanced
expressed
inhibits
to
present
findings
provide
better
understanding
mechanisms
underlying
will
facilitate
development
therapeutic
strategies
targeting
EndoMT‐driven
metastasis.
Язык: Английский
Laminin and hyaluronan supplementation of collagen hydrogels enhances endothelial function and tight junction expression on three-dimensional cylindrical microvessel-on-a-chip
Biochemical and Biophysical Research Communications,
Год журнала:
2024,
Номер
724, С. 150234 - 150234
Опубликована: Июнь 6, 2024
Vasculature-on-chip
(VoC)
models
have
become
a
prominent
tool
in
the
study
of
microvasculature
functions
because
their
cost-effective
and
ethical
production
process.
These
typically
use
hydrogel
which
three-dimensional
(3D)
microvascular
structure
is
embedded.
Thus,
VoCs
are
directly
impacted
by
physical
chemical
cues
supporting
hydrogel.
Endothelial
cell
(EC)
response
critical,
especially
organ-specific
vasculature
models,
ECs
exhibit
specific
traits
behaviors
that
vary
between
organs.
Many
studies
customize
stimuli
perceive
different
ways;
however,
customizing
composition
accordingly
to
target
organ's
extracellular
matrix
(ECM),
we
believe
has
great
potential,
been
rarely
investigated.
We
explored
this
approach
fabricating
microvessels
(MVs)
with
either
human
umbilical
vein
or
brain
3D
cylindrical
VoC
using
collagen
alone
one
supplemented
laminin
hyaluronan,
components
found
ECM.
characterized
properties
these
hydrogels
analyzed
barrier
MVs.
Barrier
function
tight
junction
(ZO-1)
expression
improved
addition
hyaluronan
composite
Язык: Английский
Decreased BIRC5-206 promotes epithelial–mesenchymal transition in nasopharyngeal carcinoma through sponging miR-145-5p
Open Medicine,
Год журнала:
2024,
Номер
19(1)
Опубликована: Янв. 1, 2024
Metastasis
significantly
contributes
to
the
poor
prognosis
of
advanced
nasopharyngeal
carcinoma
(NPC).
Our
prior
studies
have
demonstrated
a
decrease
in
BIRC5-206
expression
NPC,
which
promotes
disease
progression.
However,
role
invasion
and
metastasis
NPC
has
not
been
fully
elucidated.
In
this
study,
our
objective
was
explore
biological
function
underlying
mechanisms
NPC.
Additionally,
we
established
an
mouse
model
lung
invasiveness
using
C666
cells
assess
impact
on
metastasis.
results
revealed
that
silencing
inhibited
apoptosis
enhanced
cells,
whereas
its
overexpression
reversed
these
effects.
Moreover,
decreased
increased
N-cadherin
Vimentin
while
reducing
E-cadherin
occludin
levels,
both
Язык: Английский