Meiotic divisions and round spermatid formation do not require centriole duplication in mice DOI Creative Commons
Marnie W. Skinner,

Paula B Nhan,

Carter J. Simington

и другие.

PLoS Genetics, Год журнала: 2025, Номер 21(4), С. e1011698 - e1011698

Опубликована: Апрель 28, 2025

Centrosomes, composed of centrioles and pericentriolar matrix proteins, are traditionally viewed as essential microtubule-organizing centers (MTOCs) that facilitate bipolar spindle formation chromosome segregation during spermatogenesis. In this study, we investigated the role in male germ cell development by using a murine conditional knockout (cKO) Sas4 , critical component centriole biogenesis. We found while duplication was impaired cKO spermatocytes, these cells were still capable progressing through meiosis I II. Chromosome able to proceed non-centrosomal MTOC, indicating not required for meiotic divisions. However, spermatids inherited fewer than two exhibited severe defects spermiogenesis, including improper manchette formation, constricted perinuclear rings, disrupted acrosome morphology, failure form flagella. Consequently, males infertile due absence functional spermatozoa. Our findings demonstrate dispensable cells, they spermiogenesis sperm maturation. This work provides key insights into centrosomes fertility may have implications understanding certain conditions infertility associated with defects.

Язык: Английский

Meiotic divisions and round spermatid formation do not require centriole duplication in mice DOI Creative Commons
Marnie W. Skinner,

Paula B Nhan,

Carter J. Simington

и другие.

PLoS Genetics, Год журнала: 2025, Номер 21(4), С. e1011698 - e1011698

Опубликована: Апрель 28, 2025

Centrosomes, composed of centrioles and pericentriolar matrix proteins, are traditionally viewed as essential microtubule-organizing centers (MTOCs) that facilitate bipolar spindle formation chromosome segregation during spermatogenesis. In this study, we investigated the role in male germ cell development by using a murine conditional knockout (cKO) Sas4 , critical component centriole biogenesis. We found while duplication was impaired cKO spermatocytes, these cells were still capable progressing through meiosis I II. Chromosome able to proceed non-centrosomal MTOC, indicating not required for meiotic divisions. However, spermatids inherited fewer than two exhibited severe defects spermiogenesis, including improper manchette formation, constricted perinuclear rings, disrupted acrosome morphology, failure form flagella. Consequently, males infertile due absence functional spermatozoa. Our findings demonstrate dispensable cells, they spermiogenesis sperm maturation. This work provides key insights into centrosomes fertility may have implications understanding certain conditions infertility associated with defects.

Язык: Английский

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