Neurological Research, Год журнала: 2024, Номер 47(1), С. 35 - 43
Опубликована: Дек. 12, 2024
Objectives Due to the complex and unclear pathogenesis of neuropathic pain, there is a lack effective therapeutic strategy. miR-363-5p was considered great potential in mediating development which has not been confirmed with direct evidence. This study evaluated role pain animal cell models, aiming reveal target therapy pain.
Язык: Английский
CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(4)
Опубликована: Апрель 1, 2024
Abstract Aims This study aimed to investigate the potential therapeutic applications of stigmasterol for treating neuropathic pain. Methods Related mechanisms were investigated by DRG single‐cell sequencing analysis and use specific inhibitors in cellular experiments. In animal experiments, 32 male Sprague–Dawley rats randomly divided into sham operation group, CCI ibuprofen group. We performed behavioral tests, ELISA, H&E staining immunohistochemistry, western blotting. Results Cell communication reveals that after peripheral nerve injury, Schwann cells secrete IL‐34 act on CSF1R macrophages. After mRNA expression levels pathway NLRP3 inflammasome macrophages increased DRG. vitro studies demonstrated can reduce secretion LPS‐induced RSC96 cells; treatment cell‐conditioned medium (L‐S‐CM) does not induce proliferation migration RAW264.7 macrophages; L‐S‐CM reduces signaling (CSF1R, P38MAPK, NFκB) activation, ROS production. vivo experiments have verified thermal cold hyperalgesia rat chronic compressive injury (CCI) model; IL‐1β, IL‐6, TNF‐α, CCL2, SP, PGE2 serum rats; immunohistochemistry blot confirmed IL‐34/CSF1R rats. Conclusion Stigmasterol alleviates pain reducing cell‐macrophage cascade modulating axis.
Язык: Английский
Процитировано
10
Journal of Orthopaedic Translation, Год журнала: 2025, Номер 51, С. 227 - 241
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
2
International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(9), С. 3964 - 3964
Опубликована: Апрель 22, 2025
This study investigated the mechanism underlying Paeonol’s therapeutic efficacy against neuropathic pain. GSE158892 dataset data were used to conduct a scRNA-seq analysis. In cell experiments, Schwann cells and macrophages utilized examine pain pathogenesis using specific inhibitors. Thirty-two SD rats randomly divided into four groups: sham, chronic constriction injury (CCI), ibuprofen, Paeonol. Behavioral tests combined with ELISA, PCR, western blot, immunohistochemistry, immunofluorescence analyses conducted. CellChat analysis demonstrated that, following peripheral nerve injury, secreted IL-34, which interacted CSF1R on macrophages, leading infiltration activation of macrophages. Paeonol reduced IL-34 production by induced LPS. Conditioned medium from LPS-stimulated treated did not cause macrophage proliferation or migration, CSF1 pathway, ROS production. CCI rats, alleviated mechanical cold hyperalgesia, while reducing serum inflammatory mediators. Additionally, decreased expression levels CSF1R, phosphorylated ERK (p-ERK), NF-κB (p-NF-κB), components NLRP3 inflammasome in dorsal root ganglia rats. Conclusion: Alleviation treatment may be achieved inhibiting IL-34–CSF1R interaction, suppressing cell–macrophage interactions, DRG neuroinflammation.
Язык: Английский
Процитировано
1
Molecular Neurobiology, Год журнала: 2024, Номер 62(4), С. 4654 - 4676
Опубликована: Окт. 29, 2024
Язык: Английский
Процитировано
6
Biomedicine & Pharmacotherapy, Год журнала: 2025, Номер 184, С. 117920 - 117920
Опубликована: Фев. 21, 2025
Язык: Английский
Процитировано
0
European Journal of Pharmacology, Год журнала: 2025, Номер unknown, С. 177610 - 177610
Опубликована: Апрель 1, 2025
Язык: Английский
Процитировано
0
Molecules, Год журнала: 2025, Номер 30(9), С. 1874 - 1874
Опубликована: Апрель 23, 2025
Stigmasterol is a plant-derived phytosterol that has attracted considerable attention because of its diverse biological activities and potential therapeutic applications. In this review, the chemical properties, biosynthesis, effects stigmasterol are exhaustively summarized. Furthermore, anti-inflammatory, antioxidant, anticancer, neuroprotective, hypolipidemic have been discussed. Findings from various in vitro vivo studies revealed treating diseases, including cancer, diabetes, neurological disorders, inflammatory conditions. The mechanisms underlying these also discussed, particularly emphasizing regulation key signaling pathways molecular targets, to further clarify role stigmasterol. This review would provide reference for exploring utility as agent, thereby contributing improvement human health.
Язык: Английский
Процитировано
0
Neuroscience Letters, Год журнала: 2025, Номер unknown, С. 138282 - 138282
Опубликована: Июнь 1, 2025
Язык: Английский
Процитировано
0
Journal of Separation Science, Год журнала: 2025, Номер 48(6)
Опубликована: Июнь 1, 2025
ABSTRACT Deciphering the chemical basis of traditional Chinese medicine (TCM) is far‐reaching significance for revealing its pharmacodynamic mechanism, metabolic transformation, and drug development. However, complexity compositions large number isomers in TCM make it difficult to achieve comprehensive detection identification. Taking Codonopsis Radix as a case, we proposed reverse molecular assembly (RMA) analysis strategy rapid discovery potential new compounds complex system TCM: paired diagnostic product ions (pDPIs) were designed based on structural characteristics characteristic cleavage pathways taxa‐compounds. First, reported categorized into different taxa core structures. Second, pDPIs belonging certain taxon according structures, branch chains, pathways. Third, accuracy positive negative ion modes was verified by comparing mass spectrometry data, literature reports, standards. Finally, RMA used accurately identify components extract. In this study, 209 preliminarily characterized, 128 which are that have not been yet Radix. This study provides an important reference in‐depth elucidation TCM.
Язык: Английский
Процитировано
0
Exploration of Immunology, Год журнала: 2024, Номер unknown, С. 523 - 532
Опубликована: Авг. 28, 2024
Our immune system acts to protect us in times of stress and traumatic injury. As part the response, body produces various cytokines, which mediate or modulate functions. Such cytokines include tumor necrosis factor (TNF) interleukin 6 (IL-6) IL-17. These can also act on nervous influence pain perception. TNF-α triggers an inflammatory response two forms programmed cell death, apoptosis necroptosis, depending pathological state. For individuals with chronic conditions relating deficiency, actions these present as states, significantly altering quality life. One attractive potential solution for treating this linked is by signaling pathways pain-enhancing cytokines. Infliximab etanercept are TNF inhibitors that currently market use treatment pain. Secukinumab tocilizumab serve IL inhibitors, utilized a similar purpose. novel immunotherapies have shown efficacy numerous clinical studies acceptable side effect profiles. In review, we summarize pharmacological profiles drugs discuss their usage
Язык: Английский
Процитировано
1