BMC Cardiovascular Disorders, Год журнала: 2024, Номер 24(1)
Опубликована: Дек. 30, 2024
To screen Myocardial ischemia-reperfusion Injury in mice. adenosine monophate-activatedprotein kinase (AMPK) -related differentially expressed circularRNA (circRNA) MIRI model, Ampk-related circRNA network was drawn to provide possible ideas for the prevention and treatment of MIRI.
Язык: Английский
CNS Neuroscience & Therapeutics, Год журнала: 2024, Номер 30(4)
Опубликована: Апрель 1, 2024
Abstract The etiology of epilepsy is ascribed to the synchronized aberrant neuronal activity within brain. Circular RNAs (circRNAs), a class non‐coding characterized by their circular structures and covalent linkage, exert substantial influence on this phenomenon. CircRNAs possess stereotyped replication, transience, repetitiveness, paroxysm. Additionally, MicroRNA (miRNA) plays crucial role in regulation diverse pathological processes, including epilepsy. CircRNA particular significance due its ability function as competing endogenous RNA, thereby sequestering or inhibiting miRNA through binding target mRNA. Our review primarily concentrates elucidating functional roles, well underlying mechanisms, circRNA–miRNA–mRNA networks it explores potential utility these for early detection therapeutic intervention.
Язык: Английский
Процитировано
19
Clinical and Translational Medicine, Год журнала: 2024, Номер 14(11)
Опубликована: Ноя. 1, 2024
Язык: Английский
Процитировано
6
Brain and Behavior, Год журнала: 2024, Номер 14(8)
Опубликована: Авг. 1, 2024
Temporal lobe epilepsy (TLE), a prevalent neurological disorder, is associated with hippocampal oxidative stress and inflammation. A recent study reveals that the long noncoding RNA ILF3 divergent transcript (ILF3-AS1) level elevated in hippocampus of TLE patients; however, functional roles ILF3-AS1 underlying mechanisms deserve further investigation. Hence, this aimed to elucidate whether involved pathogenesis by regulating inflammation explore its mechanism vitro.
Язык: Английский
Процитировано
3
Hepatology Communications, Год журнала: 2025, Номер 9(1)
Опубликована: Янв. 1, 2025
Background: Autoimmune hepatitis (AIH) is a chronic liver disease characterized by immune-mediated inflammation. Despite its global prevalence, the pathogenesis of AIH remains poorly understood, and there lack specific biomarkers targeted treatments. This study aimed to investigate role hsa_circ_0109623, hsa-miR-146b-3p, Sortilin 1 (SORT1) in their potential as therapeutic targets. Methods: We collected tissue samples peripheral blood mononuclear cells from patients with healthy controls performed RT-PCR, western blotting, flow cytometry, other molecular biology techniques analyze expression SORT1. also used bioinformatics tools predict interaction between these molecules conducted luciferase reporter assays confirm binding. Results: hsa_circ_0109623 was significantly upregulated positively correlated inflammatory activity. found that could enhance CD4+ T-cell activation promote proinflammatory cytokines. Conversely, hsa-miR-146b-3p downregulated negatively In addition, acted sponge for inhibiting Th1 cell polarization cytokine production. SORT1 promoting expression. Furthermore, hsa_miR_146b-3p/SORT1 can regulate STAT1/STAT4 signaling pathway mediating progression AIH. Conclusions: The hsa_circ_0109623/hsa-miR-146b-3p/SORT1 axis plays crucial regulating These may serve targets Further research needed validate findings explore clinical applications.
Язык: Английский
Процитировано
0
Annals of Hematology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 10, 2025
Reactivation of fetal hemoglobin (Hb F, α2γ2) has been demonstrated to be a therapeutic strategy for patients with β-hemoglobinopathies. MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by silencing RNA. Both coding and non-coding can compete the same miRNAs, acting as competing endogenous (ceRNAs). However, role ceRNAs in β-thalassemia major (β-TM) their impact on γ-globin remains poorly understood. In this study, we conducted transcriptome sequencing collect circularRNA (circRNA), miRNA, mRNAs from β-TM healthy individuals. Through bioinformatics analysis, constructed GATA2‑associated ceRNA network, emphasizing hsa_circ_0005245_hsa-miR-425-3p_GATA2 pathway. Validation using qRT-PCR analysis samples, RNA immunoprecipitation, dual-luciferase reporter assays confirmed Furthermore, overexpression hsa_circ_0005245, hsa-miR-425-3p, GATA2 HUDEP-2 cells individually resulted elevated levels. Our findings identify novel pathway regulates expression, providing potential insights clinical management patients.
Язык: Английский
Процитировано
0
Animals, Год журнала: 2025, Номер 15(4), С. 581 - 581
Опубликована: Фев. 18, 2025
N6-methyladenosine (m6A) is one of the most abundant modifications in eukaryotic RNA molecules and mediates functional exertion molecules. We characterized circPAPPA validated its potential m6A modification sites secondary hair follicles (SHFs) cashmere goats. Furthermore, we generated integrated regulatory networks along with enrichment analysis signaling pathways. also explored relationship expression first intron methylation PAPPA gene SHFs Host source revealed that derived from complete exon 2 gene, spliced reverse orientation, predominantly localized cytoplasm SHF stem cells The was verified to contain at least four goats, including m6A-450/456, m6A-852, m6A-900, m6A-963. network indicated complex diverse relationships m6A-circPAPPA putative molecules, miRNAs, mRNAs, proteins. Enrichment pathways showed might play multiple roles growth development goats through mediated by target miRNAs likely significantly involved dynamic goat SHFs. Results this study provided novel information elucidate biological fiber.
Язык: Английский
Процитировано
0
Archives of Physiology and Biochemistry, Год журнала: 2025, Номер unknown, С. 1 - 11
Опубликована: Фев. 23, 2025
Diabetic retinopathy (DR) is the leading manifestation of diabetic microangiopathy. However, effective biomarkers and therapies are lacking. Circular RNAs (circRNAs) have been implicated in various diseases including DR. role circRNAs DR remains elusive. In present study, circNXN was upregulated high glucose (HG)-treated human retinal microvascular endothelial cells (hRMECs). knockdown inhibited proliferation, migration, angiogenesis hRMECs promoted apoptosis. addition, acted as a sponge for miR-338-3p to facilitate FGFR1 (fibroblast growth factor receptor 1) expression. Furthermore, rescue assays revealed that reduced promoting effect on induced by could be reversed inhibitor HG-treated hRMECs. Additionally, rat model, downregulation ameliorated vasculature changes. Our findings reveal new therapeutic strategy may provide approach clinical therapy.
Язык: Английский
Процитировано
0
Human Cell, Год журнала: 2025, Номер 38(3)
Опубликована: Апрель 9, 2025
Язык: Английский
Процитировано
0
Renal Failure, Год журнала: 2025, Номер 47(1)
Опубликована: Апрель 11, 2025
Diabetic kidney disease (DKD), characterized by mesangial fibrosis and renal dysfunction, is a major microvascular complication of diabetes. Studies have shown that miRNAs are closely related to the progression DKD. Therefore, in this study, we aimed explore whether miR-1225-3p can regulate Smad ubiquitin regulatory factor 2 (SMURF2)-mediated carbohydrate response element binding protein (ChREBP) ubiquitination through Rho GTPase-activating 5 (ARHGAP5) affect DKD mice were established intraperitoneally injecting streptozocin (STZ), cell model was generated culturing media supplemented with 25 mmol/L glucose (high glucose, HG). StarBase used predict target sites between ARHGAP5, dual-luciferase reporter gene assay verify relationship. Western blotting, RT-qPCR, flow cytometry, immunoprecipitation, ELISAs, HE staining, Masson staining detect relevant indicators. ARHGAP5 SMURF2 expression decreased, but ChREBP highly expressed tissue HG-induced mouse cells (MMCs). could transcription an association revealed. facilitated oxidative stress MCCs inhibiting ARHGAP5. In addition, promoted HA-ChREBP, mediating ARHGAP5/SMURF2-mediated MCCs. Furthermore, inhibitor inhibited inflammation tissues mice. facilitates ChREBP.
Язык: Английский
Процитировано
0
Frontiers in Molecular Biosciences, Год журнала: 2025, Номер 12
Опубликована: Апрель 14, 2025
Objectives Systemic Lupus Erythematosus (SLE) is a highly heterogeneous autoimmune disease with complex pathogenic mechanisms. Mitochondrial function and programmed cell death (PCD) play important roles in SLE. This study aims to screen biomarkers related mitochondrial SLE analyze their underlying Methods SLE-related databases were derived from the GEO database, where three merged into one database as training set. Genes PCD sourced MitoCarta 3.0 database. Key genes identified through bioinformatics machine learning, expression levels diagnostic efficacy validated using two datasets validation The relationship between immune cells was analyzed CIBERSORT infiltration analysis. Diagnostic genes-related miRNAs predicted online databases. Differential circRNAs screened circRNA datasets, circbank, finally constructing circRNA-miRNA-mRNA ceRNA regulatory network. Results From 448 differential set, key genes, IFI27 LAMP3, learning WGCNA. Enrichment analysis revealed that they mainly enriched pathways such cycle, systemic lupus erythematosus, cytosolic DNA sensing pathway, toll-like receptor (TLR) signaling pathway nod-like (NLR) pathway. Immune found compared normal group, 11 differentially expressed, 9 types of LAMP3 10 cells. final constructed network consists 2 mRNAs, 5 miRNAs, 4 circRNAs. Conclusion Our ultimately (IFI27 LAMP3) an role In future, have potential become diagnosis treatment Their response may provide new strategies for
Язык: Английский
Процитировано
0