BMC Cardiovascular Disorders,
Год журнала:
2024,
Номер
24(1)
Опубликована: Дек. 30, 2024
To
screen
Myocardial
ischemia-reperfusion
Injury
in
mice.
adenosine
monophate-activatedprotein
kinase
(AMPK)
-related
differentially
expressed
circularRNA
(circRNA)
MIRI
model,
Ampk-related
circRNA
network
was
drawn
to
provide
possible
ideas
for
the
prevention
and
treatment
of
MIRI.
CNS Neuroscience & Therapeutics,
Год журнала:
2024,
Номер
30(4)
Опубликована: Апрель 1, 2024
Abstract
The
etiology
of
epilepsy
is
ascribed
to
the
synchronized
aberrant
neuronal
activity
within
brain.
Circular
RNAs
(circRNAs),
a
class
non‐coding
characterized
by
their
circular
structures
and
covalent
linkage,
exert
substantial
influence
on
this
phenomenon.
CircRNAs
possess
stereotyped
replication,
transience,
repetitiveness,
paroxysm.
Additionally,
MicroRNA
(miRNA)
plays
crucial
role
in
regulation
diverse
pathological
processes,
including
epilepsy.
CircRNA
particular
significance
due
its
ability
function
as
competing
endogenous
RNA,
thereby
sequestering
or
inhibiting
miRNA
through
binding
target
mRNA.
Our
review
primarily
concentrates
elucidating
functional
roles,
well
underlying
mechanisms,
circRNA–miRNA–mRNA
networks
it
explores
potential
utility
these
for
early
detection
therapeutic
intervention.
Brain and Behavior,
Год журнала:
2024,
Номер
14(8)
Опубликована: Авг. 1, 2024
Temporal
lobe
epilepsy
(TLE),
a
prevalent
neurological
disorder,
is
associated
with
hippocampal
oxidative
stress
and
inflammation.
A
recent
study
reveals
that
the
long
noncoding
RNA
ILF3
divergent
transcript
(ILF3-AS1)
level
elevated
in
hippocampus
of
TLE
patients;
however,
functional
roles
ILF3-AS1
underlying
mechanisms
deserve
further
investigation.
Hence,
this
aimed
to
elucidate
whether
involved
pathogenesis
by
regulating
inflammation
explore
its
mechanism
vitro.
Hepatology Communications,
Год журнала:
2025,
Номер
9(1)
Опубликована: Янв. 1, 2025
Background:
Autoimmune
hepatitis
(AIH)
is
a
chronic
liver
disease
characterized
by
immune-mediated
inflammation.
Despite
its
global
prevalence,
the
pathogenesis
of
AIH
remains
poorly
understood,
and
there
lack
specific
biomarkers
targeted
treatments.
This
study
aimed
to
investigate
role
hsa_circ_0109623,
hsa-miR-146b-3p,
Sortilin
1
(SORT1)
in
their
potential
as
therapeutic
targets.
Methods:
We
collected
tissue
samples
peripheral
blood
mononuclear
cells
from
patients
with
healthy
controls
performed
RT-PCR,
western
blotting,
flow
cytometry,
other
molecular
biology
techniques
analyze
expression
SORT1.
also
used
bioinformatics
tools
predict
interaction
between
these
molecules
conducted
luciferase
reporter
assays
confirm
binding.
Results:
hsa_circ_0109623
was
significantly
upregulated
positively
correlated
inflammatory
activity.
found
that
could
enhance
CD4+
T-cell
activation
promote
proinflammatory
cytokines.
Conversely,
hsa-miR-146b-3p
downregulated
negatively
In
addition,
acted
sponge
for
inhibiting
Th1
cell
polarization
cytokine
production.
SORT1
promoting
expression.
Furthermore,
hsa_miR_146b-3p/SORT1
can
regulate
STAT1/STAT4
signaling
pathway
mediating
progression
AIH.
Conclusions:
The
hsa_circ_0109623/hsa-miR-146b-3p/SORT1
axis
plays
crucial
regulating
These
may
serve
targets
Further
research
needed
validate
findings
explore
clinical
applications.
Annals of Hematology,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 10, 2025
Reactivation
of
fetal
hemoglobin
(Hb
F,
α2γ2)
has
been
demonstrated
to
be
a
therapeutic
strategy
for
patients
with
β-hemoglobinopathies.
MicroRNAs
(miRNAs)
are
small
noncoding
RNAs
that
regulate
gene
expression
by
silencing
RNA.
Both
coding
and
non-coding
can
compete
the
same
miRNAs,
acting
as
competing
endogenous
(ceRNAs).
However,
role
ceRNAs
in
β-thalassemia
major
(β-TM)
their
impact
on
γ-globin
remains
poorly
understood.
In
this
study,
we
conducted
transcriptome
sequencing
collect
circularRNA
(circRNA),
miRNA,
mRNAs
from
β-TM
healthy
individuals.
Through
bioinformatics
analysis,
constructed
GATA2‑associated
ceRNA
network,
emphasizing
hsa_circ_0005245_hsa-miR-425-3p_GATA2
pathway.
Validation
using
qRT-PCR
analysis
samples,
RNA
immunoprecipitation,
dual-luciferase
reporter
assays
confirmed
Furthermore,
overexpression
hsa_circ_0005245,
hsa-miR-425-3p,
GATA2
HUDEP-2
cells
individually
resulted
elevated
levels.
Our
findings
identify
novel
pathway
regulates
expression,
providing
potential
insights
clinical
management
patients.
Animals,
Год журнала:
2025,
Номер
15(4), С. 581 - 581
Опубликована: Фев. 18, 2025
N6-methyladenosine
(m6A)
is
one
of
the
most
abundant
modifications
in
eukaryotic
RNA
molecules
and
mediates
functional
exertion
molecules.
We
characterized
circPAPPA
validated
its
potential
m6A
modification
sites
secondary
hair
follicles
(SHFs)
cashmere
goats.
Furthermore,
we
generated
integrated
regulatory
networks
along
with
enrichment
analysis
signaling
pathways.
also
explored
relationship
expression
first
intron
methylation
PAPPA
gene
SHFs
Host
source
revealed
that
derived
from
complete
exon
2
gene,
spliced
reverse
orientation,
predominantly
localized
cytoplasm
SHF
stem
cells
The
was
verified
to
contain
at
least
four
goats,
including
m6A-450/456,
m6A-852,
m6A-900,
m6A-963.
network
indicated
complex
diverse
relationships
m6A-circPAPPA
putative
molecules,
miRNAs,
mRNAs,
proteins.
Enrichment
pathways
showed
might
play
multiple
roles
growth
development
goats
through
mediated
by
target
miRNAs
likely
significantly
involved
dynamic
goat
SHFs.
Results
this
study
provided
novel
information
elucidate
biological
fiber.
Archives of Physiology and Biochemistry,
Год журнала:
2025,
Номер
unknown, С. 1 - 11
Опубликована: Фев. 23, 2025
Diabetic
retinopathy
(DR)
is
the
leading
manifestation
of
diabetic
microangiopathy.
However,
effective
biomarkers
and
therapies
are
lacking.
Circular
RNAs
(circRNAs)
have
been
implicated
in
various
diseases
including
DR.
role
circRNAs
DR
remains
elusive.
In
present
study,
circNXN
was
upregulated
high
glucose
(HG)-treated
human
retinal
microvascular
endothelial
cells
(hRMECs).
knockdown
inhibited
proliferation,
migration,
angiogenesis
hRMECs
promoted
apoptosis.
addition,
acted
as
a
sponge
for
miR-338-3p
to
facilitate
FGFR1
(fibroblast
growth
factor
receptor
1)
expression.
Furthermore,
rescue
assays
revealed
that
reduced
promoting
effect
on
induced
by
could
be
reversed
inhibitor
HG-treated
hRMECs.
Additionally,
rat
model,
downregulation
ameliorated
vasculature
changes.
Our
findings
reveal
new
therapeutic
strategy
may
provide
approach
clinical
therapy.
Diabetic
kidney
disease
(DKD),
characterized
by
mesangial
fibrosis
and
renal
dysfunction,
is
a
major
microvascular
complication
of
diabetes.
Studies
have
shown
that
miRNAs
are
closely
related
to
the
progression
DKD.
Therefore,
in
this
study,
we
aimed
explore
whether
miR-1225-3p
can
regulate
Smad
ubiquitin
regulatory
factor
2
(SMURF2)-mediated
carbohydrate
response
element
binding
protein
(ChREBP)
ubiquitination
through
Rho
GTPase-activating
5
(ARHGAP5)
affect
DKD
mice
were
established
intraperitoneally
injecting
streptozocin
(STZ),
cell
model
was
generated
culturing
media
supplemented
with
25
mmol/L
glucose
(high
glucose,
HG).
StarBase
used
predict
target
sites
between
ARHGAP5,
dual-luciferase
reporter
gene
assay
verify
relationship.
Western
blotting,
RT-qPCR,
flow
cytometry,
immunoprecipitation,
ELISAs,
HE
staining,
Masson
staining
detect
relevant
indicators.
ARHGAP5
SMURF2
expression
decreased,
but
ChREBP
highly
expressed
tissue
HG-induced
mouse
cells
(MMCs).
could
transcription
an
association
revealed.
facilitated
oxidative
stress
MCCs
inhibiting
ARHGAP5.
In
addition,
promoted
HA-ChREBP,
mediating
ARHGAP5/SMURF2-mediated
MCCs.
Furthermore,
inhibitor
inhibited
inflammation
tissues
mice.
facilitates
ChREBP.
Frontiers in Molecular Biosciences,
Год журнала:
2025,
Номер
12
Опубликована: Апрель 14, 2025
Objectives
Systemic
Lupus
Erythematosus
(SLE)
is
a
highly
heterogeneous
autoimmune
disease
with
complex
pathogenic
mechanisms.
Mitochondrial
function
and
programmed
cell
death
(PCD)
play
important
roles
in
SLE.
This
study
aims
to
screen
biomarkers
related
mitochondrial
SLE
analyze
their
underlying
Methods
SLE-related
databases
were
derived
from
the
GEO
database,
where
three
merged
into
one
database
as
training
set.
Genes
PCD
sourced
MitoCarta
3.0
database.
Key
genes
identified
through
bioinformatics
machine
learning,
expression
levels
diagnostic
efficacy
validated
using
two
datasets
validation
The
relationship
between
immune
cells
was
analyzed
CIBERSORT
infiltration
analysis.
Diagnostic
genes-related
miRNAs
predicted
online
databases.
Differential
circRNAs
screened
circRNA
datasets,
circbank,
finally
constructing
circRNA-miRNA-mRNA
ceRNA
regulatory
network.
Results
From
448
differential
set,
key
genes,
IFI27
LAMP3,
learning
WGCNA.
Enrichment
analysis
revealed
that
they
mainly
enriched
pathways
such
cycle,
systemic
lupus
erythematosus,
cytosolic
DNA
sensing
pathway,
toll-like
receptor
(TLR)
signaling
pathway
nod-like
(NLR)
pathway.
Immune
found
compared
normal
group,
11
differentially
expressed,
9
types
of
LAMP3
10
cells.
final
constructed
network
consists
2
mRNAs,
5
miRNAs,
4
circRNAs.
Conclusion
Our
ultimately
(IFI27
LAMP3)
an
role
In
future,
have
potential
become
diagnosis
treatment
Their
response
may
provide
new
strategies
for