Molecular Biology Reports, Год журнала: 2022, Номер 49(3), С. 1955 - 1971
Опубликована: Янв. 7, 2022
Язык: Английский
Molecular Cancer, Год журнала: 2020, Номер 19(1)
Опубликована: Сен. 11, 2020
Triple negative breast cancer (TNBC) remains the most challenging subtype so far. Specific therapeutic approaches have rarely achieved clinical improvements in treatment of TNBC patients and effective molecular biomarkers are largely unknown.We used paired samples high throughput RNA sequencing to identify differentially expressed circRNAs. Sucrose gradient polysome fractionation assay, antibody Mass spectra were validate active circRNA translation. The novel protein function was validated vitro vivo by gain or loss assays. Mechanistic results concluded immunoprecipitation analyses kinase activity assay.Circular HER2 (circ-HER2) encoded a protein, HER2-103. Unexpectedly, while mRNA barely detected, circ-HER2/HER2-103 ~ 30% samples. Circ-HER2/HER2-103 positive harbored worse overall prognosis than patients. Knockdown circ-HER2 inhibited cells proliferation, invasion tumorigenesis vivo, suggesting critical role tumorigenicity. Mechanistically, HER2-103 promoted homo/hetero dimerization epidermal growth factor receptor (EGFR)/HER3, sustained AKT phosphorylation downstream malignant phenotypes. Furthermore, shared same amino acid sequences as CR1 domain which could be antagonized Pertuzumab, antibody. Pertuzumab markedly attenuated tumorigenicity expressing but showed no effects cells.Our not only demonstrated that certain TNBCs truly 'HER2 negative' also highlighted implications
Язык: Английский
Процитировано
126
Molecular Therapy — Nucleic Acids, Год журнала: 2021, Номер 25, С. 613 - 637
Опубликована: Авг. 19, 2021
Breast cancer is a malignant tumor that has high mortality rate and mostly occurs in women. Although significant progress been made the implementation of personalized treatment strategies for molecular subtypes breast cancer, therapeutic response often not satisfactory. Studies have reported long non-coding RNAs (lncRNAs) are abnormally expressed closely related to occurrence development cancer. In addition, tissue cell-type specificity makes lncRNAs particularly attractive as diagnostic biomarkers, prognostic factors, specific targets. Therefore, an in-depth understanding regulatory mechanisms essential developing new strategies. this review, we systematically elucidate general characteristics, potential mechanisms, targeted therapy discuss emerging functions Additionally, also highlight advantages challenges using biomarkers diagnosis or targets drug resistance present future perspectives clinical practice.
Язык: Английский
Процитировано
81
Oncology Letters, Год журнала: 2021, Номер 22(1)
Опубликована: Май 5, 2021
Breast cancer is the most prevalent in women worldwide. Triple‑negative breast (TNBC) characterized by lack of expression estrogen receptor, progesterone and human epidermal growth factor receptor 2. It aggressive subtype accounts for 12‑20% all cases. TNBC associated with younger age onset, greater metastatic potential, higher incidence relapse, lower overall survival rates. Based on molecular phenotype, has been classified into six subtypes (BL1, BL2, M, MES, LAR, IM). treatment challenging due to its heterogeneity, highly invasive nature, relatively poor therapeutics response. Chemotherapy radiotherapy are conventional strategies TNBC. Recent research mechanistic understanding disease pathogenesis using cutting‑edge technologies led unfolding new lines therapies that have incorporated clinical practice. Poly (ADP‑ribose) polymerase immune checkpoint inhibitors made their way current paradigm. This review focuses classification, features, progress Histological connected recurrence, classification TNBC, targeted therapy early advanced advances non‑coding RNA key highlights this review.
Язык: Английский
Процитировано
75
Seminars in Cancer Biology, Год журнала: 2021, Номер 82, С. 11 - 25
Опубликована: Март 18, 2021
Tumors consist of heterogeneous cell populations, and tumor heterogeneity plays key roles in regulating tumorigenesis, metastasis, recurrence resistance to anti-tumor therapies. More more studies suggest that cancer stem cells (CSCs) promote drug as well are the major source for cells. CD24-CD44+ ALDH+ most common markers breast (BCSCs). Previous showed different BCSC label indicating BCSCs. Therefore, defining regulation mechanisms BCSCs is essential precisely targeting treating cancer. In this review, we summarized novel regulators existed their niches which has been discovered recent years, discussed latest corresponding treatments, will extend our understanding on plasticity, provide better prognosis prediction efficient therapeutic strategies
Язык: Английский
Процитировано
65
Seminars in Cancer Biology, Год журнала: 2021, Номер 82, С. 26 - 34
Опубликована: Июнь 18, 2021
Язык: Английский
Процитировано
64
npj Breast Cancer, Год журнала: 2024, Номер 10(1)
Опубликована: Янв. 27, 2024
Triple-negative breast cancer (TNBC) is generally regarded as the most aggressive subtype among cancers, but exhibits higher chemotherapeutic and immunotherapeutic responses due to its unique immunogenicity. Thus, appropriate discrimination of subtypes critical for guiding therapeutic options in clinical practice. In this research, using multiple in-house public cohorts, we investigated expression features immuno-correlations B7-H3 checked anti-tumor effect monoclonal antibody a mouse model. We also developed novel classifier combining PD-L1 TNBC. was revealed be related immuno-cold accumulated collagen addition, targeting significantly suppressed TNBC growth, reversed armored-cold phenotype, boosted anti-PD-1 immunotherapy. patients with high low showed lowest immune infiltration, highest level, therapies, which mostly belong tumors. Overall, research provides subtyping strategy based on combination B7-H3/PD-L1 expression, leads approach management
Язык: Английский
Процитировано
17
Cellular & Molecular Biology Letters, Год журнала: 2024, Номер 29(1)
Опубликована: Март 26, 2024
Abstract Ferroptosis, a therapeutic strategy for tumours, is regulated cell death characterised by the increased accumulation of iron-dependent lipid peroxides (LPO). Tumour-associated long non-coding RNAs (lncRNAs), when combined with traditional anti-cancer medicines or radiotherapy, can improve efficacy and decrease mortality in cancer. Investigating role ferroptosis-related lncRNAs may help strategise new options breast cancer (BC). Herein, we briefly discuss genes pathways ferroptosis involved iron reactive oxygen species (ROS) metabolism, including X C − /GSH/GPX4 system, ACSL4/LPCAT3/15-LOX FSP1/CoQ10/NAD(P)H pathways, investigate correlation between LncRNA BC to determine possible biomarkers related ferroptosis.
Язык: Английский
Процитировано
11
Epigenomics, Год журнала: 2019, Номер 11(10), С. 1163 - 1176
Опубликована: Июль 24, 2019
Aim: To investigate the role of circRNAs in triple-negative breast cancer (TNBC) and underlying mechanisms. Materials & methods: We performed circRNA microarrays to explore expression profiles TNBC cell lines. Experiments vitro vivo were conducted effects circPLK1 on tumor proliferation metastasis as well interaction between circPLK1, miR-296-5p PLK1 TNBC. Results conclusion: CircPLK1 was significantly upregulated associated with poor survivals. knockdown inhibited growth invasion occurrence vivo. CircPLK1-miR-296-5p-PLK1 axis regulates progression by ceRNA mechanism TNBC, indicating that may serve a prognostic factor novel therapeutic target for
Язык: Английский
Процитировано
71
Canadian Journal of Gastroenterology and Hepatology, Год журнала: 2021, Номер 2021, С. 1 - 10
Опубликована: Май 30, 2021
Intrahepatic cholestasis of pregnancy (ICP) is a pregnancy-specific liver disease. The maternal symptoms are characterized by skin pruritus and elevated bile acids, causing several adverse outcomes for fetuses, including an increased risk preterm birth, meconium-stained amniotic fluid, neonatal depression, respiratory distress syndrome, stillbirth. Genetic, hormonal, immunological, environmental factors contribute to the pathogenesis ICP, estrogen-bile acid axis thought play dominant role. advances in past 10 years uncover more details this axis. Moreover, dysregulation extracellular matrix oxygen supply, organelle dysfunction, epigenetic changes also found cause illuminating potential drug targets interfering with. Here, we summarize molecular ICP with emphasis on advancement years, aiming give updated full view field.
Язык: Английский
Процитировано
56
Cell Proliferation, Год журнала: 2020, Номер 53(5)
Опубликована: Апрель 6, 2020
Abstract Triple‐negative breast cancer (TNBC) is one of the most aggressive subtypes with negativity for oestrogen receptor (ER), progesterone (PR) and human epidermal growth factor (HER2). Non‐coding RNAs (ncRNAs) make up transcriptome are widely present in eukaryotic cells. In recent years, emerging evidence suggests that ncRNAs, mainly microRNAs (miRNAs), long ncRNAs (lncRNAs) circular (circRNAs), play prominent roles tumorigenesis development TNBC, but functions have not been fully described. this review, we systematically elucidate general characteristics biogenesis miRNAs, lncRNAs circRNAs, discuss these TNBC future perspectives clinical practice.
Язык: Английский
Процитировано
52