The Identification of Key Genes and Biological Pathways in Cardiac Arrest by Integrated Bioinformatics and Next Generation Sequencing Data Analysis DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 19, 2024

Abstract Cardiac arrest (CA) is a common cause of death world wide. The disease has lacks effective treatment. Efforts have been made to elucidate the molecular pathogenesis CA, but mechanisms remain elusive. To identify key genes and pathways in next generation sequencing (NGS) GSE200117 dataset was downloaded from Gene Expression Omnibus (GEO) database. DESeq2 tool used recognize differentially expressed (DEGs). ontology (GO) REACTOME pathway enrichment analyses were performed analyze DEGs associated signal g:Profiler IID database construct protein-protein interaction (PPI) network, modules analysis using Cytoscape. A miRNA-hub gene regulatory network TF-hub then constructed screen miRNAs, TFs hub by miRNet NetworkAnalyst Cityscape software. Receiver operating characteristic curve (ROC) verified genes. In total, 844 identified, comprising 414 up regulated 430 down GO indicated that for CA mainly enriched organonitrogen compound metabolic process, response stimulus, translation immune system. Ten (up-regulated: HSPA8, HOXA1, INCA1 TP53; down-regulated: HSPB1, LMNA, SNCA, ADAMTSL4 PDLIM7) screened. We also predicted miRNAs (hsa-mir-1914-5p hsa-mir-598-3p) (JUN PRRX2) targeting This study uses series bioinformatics technologies obtain hug genes, TFs, related CA. These results provide us with new ideas finding biomarkers treatment methods

Язык: Английский

Advances in the study of S100A9 in cardiovascular diseases DOI Creative Commons
Fengling Chen, Ziyu He, Chengming Wang

и другие.

Cell Proliferation, Год журнала: 2024, Номер 57(8)

Опубликована: Март 19, 2024

Abstract Cardiovascular disease (CVD) is a group of diseases that primarily affect the heart or blood vessels, with high disability and mortality rates, posing serious threat to human health. The causative factors, pathogenesis, characteristics common CVD differ, but they all involve pathological processes such as inflammation, oxidative stress, fibrosis. S100A9 belongs S100 family calcium‐binding proteins, which are mainly secreted by myeloid cells bind Toll‐like receptor 4 for advanced glycation end products involved in regulating inflammatory response, fibrosis, vascular calcification, endothelial barrier function CVD. latest research has found key biomarker diagnosing predicting various Therefore, this article reviews progress on diagnostic predictive, therapeutic value inflammatory‐related atherosclerosis, myocardial infarction, arterial aneurysm summarizes its molecular mechanisms progression CVD, aiming explore new predictive methods identify potential intervention targets clinical practice.

Язык: Английский

Процитировано

6

Role of S100A1, S100A4, S100A8/A9 and S100B in myocardial infarction and heart failure DOI
Bin Cheng, Yun‐Fei Bian, Xiaofei Song

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 151, С. 114348 - 114348

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

Identification of regulator gene and pathway in myocardial ischemia-reperfusion injury: a bioinformatics and biological validation study DOI Creative Commons
Yanqi Liu, Xiaodong Sheng,

Zhenghong Zhao

и другие.

Hereditas, Год журнала: 2025, Номер 162(1)

Опубликована: Март 11, 2025

Abstract Background Acute myocardial infarction (AMI) is the primary cause of cardiac mortality worldwide. However, ischemia-reperfusion injury (MIRI) following reperfusion therapy common in AMI, causing damage and affecting patient’s prognosis. Presently, there are no effective treatments available for MIRI. Methods We performed a comprehensive bioinformatics analysis using three GEO datasets on differentially expressed genes, including gene ontology (GO), pathway enrichment analyses, protein-protein interaction (PPI) network analysis. Cytoscape LASSO methods were employed to identify novel regulator genes (I/R). Notably, S100A9 was identified as potential I/R. Additionally, clinical sample analyzed prove expression mechanism its down The correlation with events also examined enhance reliability our results. Results 135 differential between peripheral blood 47 controls 92 I/R patients. distinguished diagnostic potential. RT-qPCR test demonstrated significant upregulation verified that strongly correlates left ventricular ejection fraction (LVEF) Conclusion This study confirms key progression may participate by upregulating RAGE /NFKB-NLRP3 activation. Elevated levels serve marker identifying high-risk MIRI patients, especially those coronary artery no-reflow (CNR), who might benefit from targeted therapeutic interventions. Furthermore, Peripheral AMI represents new target preventing

Язык: Английский

Процитировано

0

Semaglutide attenuates myocardial ischemia-reperfusion injury by inhibiting ferroptosis of cardiomyocytes via activation of PKC-S100A9 axis DOI Creative Commons
Yan Liu, Zixuan Li, Xiaomeng Xu

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 16

Опубликована: Март 20, 2025

Objective The incidence of ischemic cardiomyopathy increases annually worldwide, and it is the leading cause mortality in China. Although interventional diagnostic therapeutic techniques can promptly open culprit vessels, myocardial ischemia-reperfusion injury (MIRI), resulting from restored blood flow, often inevitable. Semaglutide (Sem), a novel GLP-1 analogue, primarily utilized managing Type 2 diabetes mellitus (T2DM). Recent research indicates that semaglutide may reduce risk major adverse cardiovascular events. Therefore, purpose this study to explore whether ameliorate MIRI its potential mechanism. Methods results : A mouse model (I/R) was created by ligating left anterior descending coronary artery (LAD) first for 45 min then reperfusing heart 24 h. Assessment cardiac function fibrosis were conducted through small animal ultrasound Masson’s staining. It observed enhanced recovery diminished I/R model. In vivo experiments, proved mitigate oxidative stress inhibit ferroptosis cardiomyocytes. RNA sequencing showed S100 calcium binding protein A9 (S100A9) target gene protect against MIRI. vitro , experiments decreased expression S100A9 activating Protein Kinase C(PKC) pathway, thus inhibiting Conclusion I/R-induced mechanism, mainly via activation PKC signaling pathway. considered as treatment option

Язык: Английский

Процитировано

0

Comprehensive analysis of single-cell and bulk transcriptome unravels immune landscape of atherosclerosis and develops a S100 family based-diagnostic model DOI

Yanfei Mo,

Yaoqi Ge, Dan Wang

и другие.

Computational Biology and Chemistry, Год журнала: 2025, Номер unknown, С. 108436 - 108436

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0

The Identification of Key Genes and Biological Pathways in Cardiac Arrest by Integrated Bioinformatics and Next Generation Sequencing Data Analysis DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 19, 2024

Abstract Cardiac arrest (CA) is a common cause of death world wide. The disease has lacks effective treatment. Efforts have been made to elucidate the molecular pathogenesis CA, but mechanisms remain elusive. To identify key genes and pathways in next generation sequencing (NGS) GSE200117 dataset was downloaded from Gene Expression Omnibus (GEO) database. DESeq2 tool used recognize differentially expressed (DEGs). ontology (GO) REACTOME pathway enrichment analyses were performed analyze DEGs associated signal g:Profiler IID database construct protein-protein interaction (PPI) network, modules analysis using Cytoscape. A miRNA-hub gene regulatory network TF-hub then constructed screen miRNAs, TFs hub by miRNet NetworkAnalyst Cityscape software. Receiver operating characteristic curve (ROC) verified genes. In total, 844 identified, comprising 414 up regulated 430 down GO indicated that for CA mainly enriched organonitrogen compound metabolic process, response stimulus, translation immune system. Ten (up-regulated: HSPA8, HOXA1, INCA1 TP53; down-regulated: HSPB1, LMNA, SNCA, ADAMTSL4 PDLIM7) screened. We also predicted miRNAs (hsa-mir-1914-5p hsa-mir-598-3p) (JUN PRRX2) targeting This study uses series bioinformatics technologies obtain hug genes, TFs, related CA. These results provide us with new ideas finding biomarkers treatment methods

Язык: Английский

Процитировано

0