Bio-characteristics, tissue expression of miR-375 in hypothalamic-pituitary-ovarian axis and its regulation in reproduction-related diseases DOI Creative Commons
Suxia Sun, Binglei Zhang,

Wanhang Jia

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 9, 2024

Our study concentrated on the expression of miRNA-375 in hypothalamic-pituitary-gonadal axis female Hu sheep. The investigation involved cloning precursor sequence miR-NA-375, followed by comparison with database entries and subsequent Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses. In our approach, we obtained ovaries, thalamus, cerebellum, brain, uterus, pituitary gland, hypothalamus, pineal gland from fertile but nonpregnant ewes. MiRNA extraction kit was used to extract miRNA above eight tissues. Real-time fluorescent quantitative polymerase chain reaction evaluate role miR-375 hy-pothalamic-pituitary-gonadal axis. results were compared those Anopheles gambiae, Apis mellifera, Bos taurus, Drosophila melanogaster, Danio rerio, Fugu rubripes, Gallus gallus, Homo sapiens, Monodelphis domestica, Macaca mulatta, Mus musculus, Pan troglodytes, Rattus norvegicus, Tetraodon nigroviridis, Xenopus tropicalis miRBase database. It found that oar-miR-375 highly conserved. Notably, significantly higher (p < 0.01) than hypothalamus. also predicting target genes. GO KEGG analyses these predicted genes revealed is 182 biological processes, affects 186 cellular components, participates 184 molecular functions. terms pathway enrichment, linked nine pathways, including Hippo, Wnt, mTOR signaling pathways. This has validated interaction between its gene FZD4, which can be recognized bound produce effects. These findings lead inference may play a crucial regulatory sheep reproduction through Hippo Wnt pathway, laying foundation for further exploration miR-375's this domain.

Язык: Английский

The Hippo signalling pathway in bone homeostasis: Under the regulation of mechanics and aging DOI Creative Commons
Zhengda Li, Junqing Lin, Jing Wu

и другие.

Cell Proliferation, Год журнала: 2024, Номер 57(10)

Опубликована: Май 3, 2024

Abstract The Hippo signalling pathway is a conserved kinase cascade that orchestrates diverse cellular processes, such as proliferation, apoptosis, lineage commitment and stemness. With the onset of society ages, research on skeletal aging‐mechanics‐bone homeostasis has exploded. In recent years, aging mechanical force in system have gained groundbreaking progress. Under regulation mechanics aging, crucial role development bone. We synthesize current knowledge pathway, particularly its downstream effectors yes‐associated protein (YAP) transcriptional co‐activator with PDZ‐binding motif (TAZ), bone homeostasis. discuss specification function different cell types by pathway. interactions other pathways, Wnt, transforming growth factor beta nuclear kappa‐B, are also mentioned because their importance for modulating Furthermore, YAP/TAZ been extensively studied mechanotransducers. Due to space limitations, we focus reviewing how forces influence fate, communications through dysregulated

Язык: Английский

Процитировано

6

The physiological and pathogenic roles of yes-associated protein/transcriptional co-activator with PDZ-binding motif in bone or skeletal motor system-related cells DOI Open Access
Yao Huang, Xueqian Ouyang, Jinghua Tan

и другие.

CytoJournal, Год журнала: 2025, Номер 22, С. 13 - 13

Опубликована: Фев. 8, 2025

Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) are the primary downstream effectors of Hippo signaling pathway. This pathway plays a crucial role in regulating organ size, maintaining tissue homeostasis, controlling cellular processes such as fate determination development. review provides an overview current understanding how regulators YAP TAZ contribute to physiological pathological tissues cells associated skeletal motor system. The underlying molecular mechanisms mechanical transduction were reviewed.

Язык: Английский

Процитировано

0

Regulation of Skeletogenic Pathways by m6A RNA Modification: A Comprehensive Review DOI Creative Commons
Ehsan Pashay Ahi

Calcified Tissue International, Год журнала: 2025, Номер 116(1)

Опубликована: Апрель 3, 2025

Язык: Английский

Процитировано

0

The Hippo pathway in bone and cartilage: implications for development and disease DOI Creative Commons
Chuan Shao, Hao Chen, Tingting Liu

и другие.

PeerJ, Год журнала: 2025, Номер 13, С. e19334 - e19334

Опубликована: Апрель 22, 2025

Bone is the main structure of human body; it mainly plays a supporting role and participates in metabolic processes. The Hippo signaling pathway composed series protein kinases, including mammalian STE20-like kinase MST1/2 large tumor suppressor LATS1/2, which are widely involved pathophysiological processes, cell proliferation, differentiation, apoptosis death, especially those related to biomechanical transduction vivo . However, regulating skeletal system development evolution bone-related diseases remains poorly understood. can intervene regulate physiological activities cells such as osteoclasts chondrocytes through its own or other pathways, Wnt pathway, Notch receptor activator nuclear factor-κB ligand (RANKL), thereby affecting occurrence bone diseases. This article discusses disease provide new insights into treatment by targeting pathway.

Язык: Английский

Процитировано

0

CCN Proteins as Matricellular Regulators of Bone in Aging and Disease DOI Creative Commons
Parveez Ahamed Abdul Azees,

R Rajesh,

Travis J. Block

и другие.

Current Osteoporosis Reports, Год журнала: 2025, Номер 23(1)

Опубликована: Май 23, 2025

This review explores the role of cell communication network (CCN) proteins in regulating skeletal physiology, aging, and disease, particularly within context balanced bone remodeling. Recent conceptualization paracrine endocrine networks marrow as a form osteoimmunological crosstalk suggests significant for matricellular signaling homeostasis. As multifunctional adapters cell-matrix interactions, CCNs are emerging focal point parathyroid hormone (PTH) regulation RANKL/RANK/OPG axis aging. Altered CCN expression creates permissive environment accelerated postmenopausal loss may contribute to pathogenesis osteoporosis other diseases related modulate fundamental mechanisms development, homeostasis repair. During dysregulation negatively affect health disease progression. result, constitute promising therapeutic targets improving maintaining aging health.

Язык: Английский

Процитировано

0

Bio-characteristics, tissue expression of miR-375 in hypothalamic-pituitary-ovarian axis and its regulation in reproduction-related diseases DOI Creative Commons
Suxia Sun, Binglei Zhang,

Wanhang Jia

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 9, 2024

Our study concentrated on the expression of miRNA-375 in hypothalamic-pituitary-gonadal axis female Hu sheep. The investigation involved cloning precursor sequence miR-NA-375, followed by comparison with database entries and subsequent Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses. In our approach, we obtained ovaries, thalamus, cerebellum, brain, uterus, pituitary gland, hypothalamus, pineal gland from fertile but nonpregnant ewes. MiRNA extraction kit was used to extract miRNA above eight tissues. Real-time fluorescent quantitative polymerase chain reaction evaluate role miR-375 hy-pothalamic-pituitary-gonadal axis. results were compared those Anopheles gambiae, Apis mellifera, Bos taurus, Drosophila melanogaster, Danio rerio, Fugu rubripes, Gallus gallus, Homo sapiens, Monodelphis domestica, Macaca mulatta, Mus musculus, Pan troglodytes, Rattus norvegicus, Tetraodon nigroviridis, Xenopus tropicalis miRBase database. It found that oar-miR-375 highly conserved. Notably, significantly higher (p < 0.01) than hypothalamus. also predicting target genes. GO KEGG analyses these predicted genes revealed is 182 biological processes, affects 186 cellular components, participates 184 molecular functions. terms pathway enrichment, linked nine pathways, including Hippo, Wnt, mTOR signaling pathways. This has validated interaction between its gene FZD4, which can be recognized bound produce effects. These findings lead inference may play a crucial regulatory sheep reproduction through Hippo Wnt pathway, laying foundation for further exploration miR-375's this domain.

Язык: Английский

Процитировано

1