Exploring the Complex Mechanisms of Isoflavones: From Cell Bioavailability, to Cell Dynamics and Breast Cancer DOI Creative Commons
François Ferrière,

Nagham Aasi,

Gilles Flouriot

и другие.

Phytotherapy Research, Год журнала: 2024, Номер unknown

Опубликована: Дек. 20, 2024

ABSTRACT In Western countries, the increase in consumption of soy‐derived products raises population's exposure to isoflavones. These molecules, present many foods, have numerous effects on body's cells, including regulation transcription and epigenetics, cell signaling, cycle, growth, apoptosis, oxidative stress. However, despite multitude studies conducted, these compounds, it remains difficult draw definitive conclusions regarding their safety or dangerousness diet. Indeed, some epidemiological highlight health benefits consuming isoflavone‐rich notably by reducing risk certain cancers. several conducted models show that molecules can negative fate, particularly with regard proliferation survival mammary tumor cells. Isoflavones are mainly genistein, daidzein, formononetin, biochanin A. belong family phytoestrogens, which capable interacting both nuclear estrogen receptor, ERα ERβ, trigger agonistic antagonistic effects. Due estrogenic properties, isoflavones suspected promote hormone‐dependent cancers such as breast cancer. This suspicion is based primarily ability bind thereby altering signaling pathways control growth. study results sometimes contradictory. Some suggest may protect against cancer acting selective receptor modulators, while others potential role stimulating review explores literature isoflavones, focusing influence ERα‐dependent

Язык: Английский

Single-cell RNA sequencing and immune microenvironment analysis reveal PLOD2-driven malignant transformation in cervical cancer DOI Creative Commons
Zhiheng Lin,

Fengxin Wang,

Renwu Yin

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 15

Опубликована: Янв. 7, 2025

Cervical cancer is the fourth most common in women globally, and main cause of disease has been found to be ongoing HPV infection. remains primary cancer-related death despite major improvements screening treatment approaches, especially low- middle-income nations. Therefore, it crucial investigate tumor microenvironment advanced cervical order identify possible targets. In better understand malignant epithelial cells (EPCs), this study used bulk RNA-seq data from UCSC conjunction with single-cell RNA sequencing ArrayExpress database. After putting quality control procedures into place, cell type identification clustering analysis using Seurat software were carried out. To clarify functional pathways, enrichment differential gene expression The CIBERSORT ESTIMATE R packages evaluate immune characteristics, univariate multivariate Cox regression analyses extract prognostic features. Furthermore, assessments drug sensitivity Eight types identified, EPCs showing high proliferative stemness Five EPC subpopulations defined, C1 NNMT+ CAEPCs driving differentiation. A NNMT Risk Score (NCRS) model was developed, revealing a correlation between elevated NCRS scores adverse patient outcomes characterized by evasion. vitro experiments validated that PLOD2 significantly enhances proliferation, migration, invasion cells. This investigation delineated eight five cancer, establishing as therapeutic target. demonstrated its capability, indicating higher are associated poorer clinical outcomes. validation highlights potential, underscoring critical need for integrating immunotherapy targeted strategies enhance diagnostic approaches cancer.

Язык: Английский

Процитировано

11
Single‐cell RNA sequencing elucidated the landscape of breast cancer brain metastases and identified ILF2 as a potential therapeutic target DOI Creative Commons
Jindong Xie, Anli Yang, Qianwen Liu

и другие.

Cell Proliferation, Год журнала: 2024, Номер 57(11)

Опубликована: Июнь 29, 2024

Abstract Distant metastasis remains the primary cause of morbidity in patients with breast cancer. Hence, development more efficacious strategies and exploration potential targets for metastatic cancer are urgently needed. The data six brain metastases (BCBrM) from two centres were collected, a comprehensive landscape entire tumour ecosystem was generated through utilisation single‐cell RNA sequencing. We utilised Monocle2 CellChat algorithms to investigate interrelationships among each subcluster. In addition, multiple signatures collected evaluate key components subclusters multi‐omics methodologies. Finally, we elucidated common expression programs malignant cells, experiments conducted vitro vivo determine functions interleukin enhancer‐binding factor 2 ( ILF2 ), which is gene module, BCBrM progression. found that major cell type exhibited diverse characteristics. Besides, our study indicated specifically associated BCBrM, experimental validations further demonstrated deficiency hindered Our offers novel perspectives on heterogeneity suggests could serve as promising biomarker or therapeutic target BCBrM.

Язык: Английский

Процитировано

16
Single-cell analysis unveils cell subtypes of acral melanoma cells at the early and late differentiation stages DOI Creative Commons

Mengyuan Hou,

Zhijie Zhao,

Songbai Zhu

и другие.

Journal of Cancer, Год журнала: 2024, Номер 16(3), С. 898 - 916

Опубликована: Дек. 31, 2024

Background: Melanoma, a malignant neoplasm originating from melanocytes, is form of skin cancer with rapidly increasing global incidence, often exacerbated by UV radiation [1].Particularly, acral melanoma, characterized its swift metastasis and poor prognosis, underscores the significance further research into heterogeneity.Single-cell sequencing has been widely utilized in study tumor heterogeneity; however, related to melanoma remains be refined. Materials Methods:We employed single-cell RNA (scRNA-seq) transcriptomic analysis delve cells six samples patients.This approach enabled identification critical cell subpopulations their roles progression.Subsequently, we examined interactions among these analyzed other types.Results: Our identified C3 ID2+ as an early-stage subpopulation C4 PCLAF+ late-stage evolution.Through our analysis, Melanoma significant (AM), playing pivotal role differentiation development AM.Further transcription factors, enriched pathways, stemness, trajectories highlighted (AM) proliferation. Conclusion:This identifies new factors influencing progression, providing foundation for subsequent research.

Язык: Английский

Процитировано

11
CD146 promotes resistance of NSCLC brain metastases to pemetrexed via the NF-κB signaling pathway DOI Creative Commons
Hao Qu, Fang Yan, Feng Zhang

и другие.

Frontiers in Pharmacology, Год журнала: 2025, Номер 15

Опубликована: Янв. 13, 2025

Introduction Pemetrexed is a first line drug for brain metastases from lung cancer, either as monotherapy or combined with other drugs. The frequent occurrence of initial and acquired resistance to pemetrexed results in limited treatment effectiveness metastases. CD146 was recently found play important roles chemoresistance tumor progression. However, the underlying mechanisms CD146’s effects remain undefined. Method Sensitivity assessed preclinical metastasis (BM) model based on adenocarcinoma PC9 cells. role mechanism non-small cell cancer (NSCLC) were explored vitro vivo . A subpopulation metastatic cells derived progenitor (PC9-BrMS) significantly resistant pemetrexed. levels increased metastases, while inhibition suppressed BM Mechanistically, mediated by promoting DNA damage repair, maintaining normal cycle progression, regulating NF-KB pathway counter apoptosis, these damage, arrest, apoptosis after well reemergence restoration. Discussion In summary, this study revealed that NSCLC PEM dependent CD146.Thus might be targeted clinic overcome NSCLC.

Язык: Английский

Процитировано

0
Single-cell insights into HNSCC tumor heterogeneity and programmed cell death pathways DOI

Yuanhao Chai,

Jianlin Zhang, Wenwen Shao

и другие.

Translational Oncology, Год журнала: 2025, Номер 54, С. 102341 - 102341

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

0
Single-cell sequencing reveals PHLDA1-positive smooth muscle cells promote local invasion in head and neck squamous cell carcinoma DOI
Bing Guo, Xutao Wen,

Shun Yu

и другие.

Translational Oncology, Год журнала: 2025, Номер 55, С. 102301 - 102301

Опубликована: Март 25, 2025

Язык: Английский

Процитировано

0
Unveiling purine metabolism dysregulation orchestrated immunosuppression in advanced pancreatic cancer and concentrating on the central role of NT5E DOI Creative Commons
Junqian Zhang, Xiaobo Zhang, R. Wu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Апрель 1, 2025

The dismal efficacy of immunotherapy for Pancreatic cancer (PC) can be predominantly ascribed to its distinctive cold-tumor properties. by-products purine metabolic reprogramming are extensively engaged in tumor immune modulation, influencing the functions and recruitment cells molding an microenvironment that is propitious growth. We harnessed single-cell transcriptomics spatial concurrently analyze metabolism (PM) features PC microenvironment. quantitatively appraised PM traits diverse cell subsets via scoring algorithms such as AUCell Ucell. Moreover, development cell-cell interaction analysis elucidated alterations TME induced by dysregulation. Additionally, we defined disorder characteristics patients utilized this assess phenotypes prognoses patient population. Also, identified crucial intermediate genes impact establishment immunosuppressive environment within PC, validated them through sectioning co-culture experiments. Multi - dimensional transcriptome data unique heterogeneity microenvironment, which manifested fibroblasts demonstrating higher scores TME. Cellchat revealed malignant with elevated expression were concomitantly associated frequent interactions CAFs well high ligand-receptor pairs transcription factors. Spatial further corroborated finding. Furthermore, newly constructed criteria indicated levels a lack response Finally, study singular role NT5E immunosuppression resulting from PC. CCK8 invasion experiments following model demonstrated intervention targeting could reverse augmented malignancy co-cultured CAFs. potentially key target reversing "stiff-cancer" This demonstrates disorders impinge upon exacerbate engendered progression fibrosis. Therapeutic strategies or may offer ray hope advanced PDAC.

Язык: Английский

Процитировано

0
The role of cancer‐associated fibroblasts in the tumour microenvironment of urinary system DOI Creative Commons

Ri Hong,

Puguang Yu,

Xiaoli Zhang

и другие.

Clinical and Translational Medicine, Год журнала: 2025, Номер 15(4)

Опубликована: Апрель 1, 2025

Abstract Urological tumours are a type of neoplasms that significantly jeopardise human life and wellbeing. Cancer‐associated fibroblasts (CAFs), serving as the primary component stromal cellular milieu, form diverse cohort exerts substantial influence on tumourigenesis tumour progression. In this review, we summarised literatures regarding functions CAFs in urinary microenvironment (TME). We primarily examined multifaceted activities TME urological system tumours, including inhibiting immunity, remodelling extracellular matrix, promoting growth, metastasis, drug resistance their clinical applications. also discussed potential future directions for leveraging artificial intelligence research. Key points The interaction with various cell secretory factors tumors. application diagnosis, treatment prognosis

Язык: Английский

Процитировано

0
Hormonal and Neuronal Interactions Shaping the Brain Metastatic Microenvironment DOI Creative Commons

Stephen Shovlin,

Leonie S. Young, Damir Varešlija

и другие.

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217739 - 217739

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Deciphering the role of ELAVL1: Insights from pan-cancer multiomics analyses with emphasis on nasopharyngeal carcinoma DOI
Jindong Xie, Yi Xie,

Wencheng Tan

и другие.

Journal of Translational Internal Medicine, Год журнала: 2025, Номер 13(2), С. 138 - 155

Опубликована: Апрель 1, 2025

Abstract Background and Objectives Cancer continues to be a predominant cause of mortality worldwide, underscoring the critical need identify develop novel biomarkers improve prognostic accuracy therapeutic approaches. The dysregulation ELAVL1 is linked various diseases, including cancer. Nevertheless, its role across different cancer types remains insufficiently investigated. Methods We conducted systematic investigation into expression patterns, significance, genomic alterations, modifications, functional implications in pan-cancer types. Besides, we performed vitro vivo experiments confirm nasopharyngeal carcinoma (NPC). Results By utilizing multi-omics datasets, found obvious overexpression at both mRNA protein levels, with malignant cells. Survival analysis revealed that increased was unfavorable outcomes certain cancers; however, effect difers among Additionally, alterations modifications were related tumor progression. discovered elevated NPC tissues. In addition, survival indicated patients higher had worse prognoses. Functional assays demonstrated suppression led decreased proliferation migration cell lines. Moreover, knockdown effectively inhibited progression lymph node lung metastasis models. Conclusions summary, exhibits diverse complex involvement Targeting it might inhibit progression, making promising biomarker target for enhancing treatment outcomes.

Язык: Английский

Процитировано

0