Phytotherapy Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 20, 2024
ABSTRACT
In
Western
countries,
the
increase
in
consumption
of
soy‐derived
products
raises
population's
exposure
to
isoflavones.
These
molecules,
present
many
foods,
have
numerous
effects
on
body's
cells,
including
regulation
transcription
and
epigenetics,
cell
signaling,
cycle,
growth,
apoptosis,
oxidative
stress.
However,
despite
multitude
studies
conducted,
these
compounds,
it
remains
difficult
draw
definitive
conclusions
regarding
their
safety
or
dangerousness
diet.
Indeed,
some
epidemiological
highlight
health
benefits
consuming
isoflavone‐rich
notably
by
reducing
risk
certain
cancers.
several
conducted
models
show
that
molecules
can
negative
fate,
particularly
with
regard
proliferation
survival
mammary
tumor
cells.
Isoflavones
are
mainly
genistein,
daidzein,
formononetin,
biochanin
A.
belong
family
phytoestrogens,
which
capable
interacting
both
nuclear
estrogen
receptor,
ERα
ERβ,
trigger
agonistic
antagonistic
effects.
Due
estrogenic
properties,
isoflavones
suspected
promote
hormone‐dependent
cancers
such
as
breast
cancer.
This
suspicion
is
based
primarily
ability
bind
thereby
altering
signaling
pathways
control
growth.
study
results
sometimes
contradictory.
Some
suggest
may
protect
against
cancer
acting
selective
receptor
modulators,
while
others
potential
role
stimulating
review
explores
literature
isoflavones,
focusing
influence
ERα‐dependent
Frontiers in Immunology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 7, 2025
Cervical
cancer
is
the
fourth
most
common
in
women
globally,
and
main
cause
of
disease
has
been
found
to
be
ongoing
HPV
infection.
remains
primary
cancer-related
death
despite
major
improvements
screening
treatment
approaches,
especially
low-
middle-income
nations.
Therefore,
it
crucial
investigate
tumor
microenvironment
advanced
cervical
order
identify
possible
targets.
In
better
understand
malignant
epithelial
cells
(EPCs),
this
study
used
bulk
RNA-seq
data
from
UCSC
conjunction
with
single-cell
RNA
sequencing
ArrayExpress
database.
After
putting
quality
control
procedures
into
place,
cell
type
identification
clustering
analysis
using
Seurat
software
were
carried
out.
To
clarify
functional
pathways,
enrichment
differential
gene
expression
The
CIBERSORT
ESTIMATE
R
packages
evaluate
immune
characteristics,
univariate
multivariate
Cox
regression
analyses
extract
prognostic
features.
Furthermore,
assessments
drug
sensitivity
Eight
types
identified,
EPCs
showing
high
proliferative
stemness
Five
EPC
subpopulations
defined,
C1
NNMT+
CAEPCs
driving
differentiation.
A
NNMT
Risk
Score
(NCRS)
model
was
developed,
revealing
a
correlation
between
elevated
NCRS
scores
adverse
patient
outcomes
characterized
by
evasion.
vitro
experiments
validated
that
PLOD2
significantly
enhances
proliferation,
migration,
invasion
cells.
This
investigation
delineated
eight
five
cancer,
establishing
as
therapeutic
target.
demonstrated
its
capability,
indicating
higher
are
associated
poorer
clinical
outcomes.
validation
highlights
potential,
underscoring
critical
need
for
integrating
immunotherapy
targeted
strategies
enhance
diagnostic
approaches
cancer.
Cell Proliferation,
Год журнала:
2024,
Номер
57(11)
Опубликована: Июнь 29, 2024
Abstract
Distant
metastasis
remains
the
primary
cause
of
morbidity
in
patients
with
breast
cancer.
Hence,
development
more
efficacious
strategies
and
exploration
potential
targets
for
metastatic
cancer
are
urgently
needed.
The
data
six
brain
metastases
(BCBrM)
from
two
centres
were
collected,
a
comprehensive
landscape
entire
tumour
ecosystem
was
generated
through
utilisation
single‐cell
RNA
sequencing.
We
utilised
Monocle2
CellChat
algorithms
to
investigate
interrelationships
among
each
subcluster.
In
addition,
multiple
signatures
collected
evaluate
key
components
subclusters
multi‐omics
methodologies.
Finally,
we
elucidated
common
expression
programs
malignant
cells,
experiments
conducted
vitro
vivo
determine
functions
interleukin
enhancer‐binding
factor
2
(
ILF2
),
which
is
gene
module,
BCBrM
progression.
found
that
major
cell
type
exhibited
diverse
characteristics.
Besides,
our
study
indicated
specifically
associated
BCBrM,
experimental
validations
further
demonstrated
deficiency
hindered
Our
offers
novel
perspectives
on
heterogeneity
suggests
could
serve
as
promising
biomarker
or
therapeutic
target
BCBrM.
Journal of Cancer,
Год журнала:
2024,
Номер
16(3), С. 898 - 916
Опубликована: Дек. 31, 2024
Background:
Melanoma,
a
malignant
neoplasm
originating
from
melanocytes,
is
form
of
skin
cancer
with
rapidly
increasing
global
incidence,
often
exacerbated
by
UV
radiation
[1].Particularly,
acral
melanoma,
characterized
its
swift
metastasis
and
poor
prognosis,
underscores
the
significance
further
research
into
heterogeneity.Single-cell
sequencing
has
been
widely
utilized
in
study
tumor
heterogeneity;
however,
related
to
melanoma
remains
be
refined.
Materials
Methods:We
employed
single-cell
RNA
(scRNA-seq)
transcriptomic
analysis
delve
cells
six
samples
patients.This
approach
enabled
identification
critical
cell
subpopulations
their
roles
progression.Subsequently,
we
examined
interactions
among
these
analyzed
other
types.Results:
Our
identified
C3
ID2+
as
an
early-stage
subpopulation
C4
PCLAF+
late-stage
evolution.Through
our
analysis,
Melanoma
significant
(AM),
playing
pivotal
role
differentiation
development
AM.Further
transcription
factors,
enriched
pathways,
stemness,
trajectories
highlighted
(AM)
proliferation.
Conclusion:This
identifies
new
factors
influencing
progression,
providing
foundation
for
subsequent
research.
Frontiers in Pharmacology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 13, 2025
Introduction
Pemetrexed
is
a
first
line
drug
for
brain
metastases
from
lung
cancer,
either
as
monotherapy
or
combined
with
other
drugs.
The
frequent
occurrence
of
initial
and
acquired
resistance
to
pemetrexed
results
in
limited
treatment
effectiveness
metastases.
CD146
was
recently
found
play
important
roles
chemoresistance
tumor
progression.
However,
the
underlying
mechanisms
CD146’s
effects
remain
undefined.
Method
Sensitivity
assessed
preclinical
metastasis
(BM)
model
based
on
adenocarcinoma
PC9
cells.
role
mechanism
non-small
cell
cancer
(NSCLC)
were
explored
vitro
vivo
.
A
subpopulation
metastatic
cells
derived
progenitor
(PC9-BrMS)
significantly
resistant
pemetrexed.
levels
increased
metastases,
while
inhibition
suppressed
BM
Mechanistically,
mediated
by
promoting
DNA
damage
repair,
maintaining
normal
cycle
progression,
regulating
NF-KB
pathway
counter
apoptosis,
these
damage,
arrest,
apoptosis
after
well
reemergence
restoration.
Discussion
In
summary,
this
study
revealed
that
NSCLC
PEM
dependent
CD146.Thus
might
be
targeted
clinic
overcome
NSCLC.
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 1, 2025
The
dismal
efficacy
of
immunotherapy
for
Pancreatic
cancer
(PC)
can
be
predominantly
ascribed
to
its
distinctive
cold-tumor
properties.
by-products
purine
metabolic
reprogramming
are
extensively
engaged
in
tumor
immune
modulation,
influencing
the
functions
and
recruitment
cells
molding
an
microenvironment
that
is
propitious
growth.
We
harnessed
single-cell
transcriptomics
spatial
concurrently
analyze
metabolism
(PM)
features
PC
microenvironment.
quantitatively
appraised
PM
traits
diverse
cell
subsets
via
scoring
algorithms
such
as
AUCell
Ucell.
Moreover,
development
cell-cell
interaction
analysis
elucidated
alterations
TME
induced
by
dysregulation.
Additionally,
we
defined
disorder
characteristics
patients
utilized
this
assess
phenotypes
prognoses
patient
population.
Also,
identified
crucial
intermediate
genes
impact
establishment
immunosuppressive
environment
within
PC,
validated
them
through
sectioning
co-culture
experiments.
Multi
-
dimensional
transcriptome
data
unique
heterogeneity
microenvironment,
which
manifested
fibroblasts
demonstrating
higher
scores
TME.
Cellchat
revealed
malignant
with
elevated
expression
were
concomitantly
associated
frequent
interactions
CAFs
well
high
ligand-receptor
pairs
transcription
factors.
Spatial
further
corroborated
finding.
Furthermore,
newly
constructed
criteria
indicated
levels
a
lack
response
Finally,
study
singular
role
NT5E
immunosuppression
resulting
from
PC.
CCK8
invasion
experiments
following
model
demonstrated
intervention
targeting
could
reverse
augmented
malignancy
co-cultured
CAFs.
potentially
key
target
reversing
"stiff-cancer"
This
demonstrates
disorders
impinge
upon
exacerbate
engendered
progression
fibrosis.
Therapeutic
strategies
or
may
offer
ray
hope
advanced
PDAC.
Clinical and Translational Medicine,
Год журнала:
2025,
Номер
15(4)
Опубликована: Апрель 1, 2025
Abstract
Urological
tumours
are
a
type
of
neoplasms
that
significantly
jeopardise
human
life
and
wellbeing.
Cancer‐associated
fibroblasts
(CAFs),
serving
as
the
primary
component
stromal
cellular
milieu,
form
diverse
cohort
exerts
substantial
influence
on
tumourigenesis
tumour
progression.
In
this
review,
we
summarised
literatures
regarding
functions
CAFs
in
urinary
microenvironment
(TME).
We
primarily
examined
multifaceted
activities
TME
urological
system
tumours,
including
inhibiting
immunity,
remodelling
extracellular
matrix,
promoting
growth,
metastasis,
drug
resistance
their
clinical
applications.
also
discussed
potential
future
directions
for
leveraging
artificial
intelligence
research.
Key
points
The
interaction
with
various
cell
secretory
factors
tumors.
application
diagnosis,
treatment
prognosis
Journal of Translational Internal Medicine,
Год журнала:
2025,
Номер
13(2), С. 138 - 155
Опубликована: Апрель 1, 2025
Abstract
Background
and
Objectives
Cancer
continues
to
be
a
predominant
cause
of
mortality
worldwide,
underscoring
the
critical
need
identify
develop
novel
biomarkers
improve
prognostic
accuracy
therapeutic
approaches.
The
dysregulation
ELAVL1
is
linked
various
diseases,
including
cancer.
Nevertheless,
its
role
across
different
cancer
types
remains
insufficiently
investigated.
Methods
We
conducted
systematic
investigation
into
expression
patterns,
significance,
genomic
alterations,
modifications,
functional
implications
in
pan-cancer
types.
Besides,
we
performed
vitro
vivo
experiments
confirm
nasopharyngeal
carcinoma
(NPC).
Results
By
utilizing
multi-omics
datasets,
found
obvious
overexpression
at
both
mRNA
protein
levels,
with
malignant
cells.
Survival
analysis
revealed
that
increased
was
unfavorable
outcomes
certain
cancers;
however,
effect
difers
among
Additionally,
alterations
modifications
were
related
tumor
progression.
discovered
elevated
NPC
tissues.
In
addition,
survival
indicated
patients
higher
had
worse
prognoses.
Functional
assays
demonstrated
suppression
led
decreased
proliferation
migration
cell
lines.
Moreover,
knockdown
effectively
inhibited
progression
lymph
node
lung
metastasis
models.
Conclusions
summary,
exhibits
diverse
complex
involvement
Targeting
it
might
inhibit
progression,
making
promising
biomarker
target
for
enhancing
treatment
outcomes.