The Sinking Dead—Arctic Deep‐Sea Scavengers' Diet Suggests Nekton as Vector in Benthopelagic Coupling DOI Creative Commons
Lara Schmittmann, Sophie V. Schindler, Till Bayer

и другие.

Environmental DNA, Год журнала: 2024, Номер 6(5)

Опубликована: Сен. 1, 2024

ABSTRACT Many benthic deep‐sea animals rely on carcasses from the overlying water column that sink to seafloor and form local organic enrichments known as food falls. This flux of carbon shallow pelagic deep sea is part biological pump (BCP) such contributes sequestration. For a complete understanding budgets, it crucial identify diversity distribution sinking which are difficult detect by observational methods. Here, we analyzed diet abundant amphipod scavenger, Eurythenes gryllus , DNA metabarcoding assess their potential falls in Fram Strait, gateway Arctic. E. scavenges nekton but so far was not certain whether this represents main diet. We detected dietary taxa (26 total) 20 out 101 amphipods. found amphipods primarily fed larger including fish, cephalopods, mammals, with bony fish being most targeted source terms abundance. Only one had gelatinous organism. These results support hypothesis targets mostly The differed between Eastern Western suggests regional variability availability. also detected, for first time infections parasitic dinoflagellate Hematodinium . detection demonstrates revealing both web dynamics host–parasite interactions sea. seems promising “natural sampler” monitor will help investigate importance medium‐sized vertical export rapidly changing Arctic Ocean.

Язык: Английский

Arctic nekton uncovered by eDNA metabarcoding: Diversity, potential range expansions, and pelagic‐benthic coupling DOI Creative Commons
Véronique Merten, Oscar Puebla, Till Bayer

и другие.

Environmental DNA, Год журнала: 2023, Номер 5(3), С. 503 - 518

Опубликована: Март 16, 2023

Abstract The Arctic Ocean is home to a unique fauna that disproportionately affected by global warming but remains under‐studied. Due their high mobility and responsiveness warming, cephalopods fishes are good indicators of the reshuffling communities. Here, we established nekton biodiversity baseline for Fram Strait, only deep connection between North Atlantic Ocean. Using universal primers (12S) (18S), amplified environmental DNA (eDNA) from seawater (50–2700 m) deep‐sea sediment samples collected at LTER HAUSGARTEN observatory. We detected 12 cephalopod 31 fish taxa in seven 28 sediment, including elusive Greenland shark ( Somniosus microcephalus ). Our data suggest three Mallotus villosus , Thunnus sp., Micromesistius poutassou ) one squid Histioteuthis sp.) range expansions. detection eDNA pelagic origin also suggests M. Arctozenus risso as well gonatid squids potential contributors carbon flux. Continuous monitoring needed understand ecosystem impacts rapid proves be suitable tool this endeavor.

Язык: Английский

Процитировано

13
Comparing eDNA metabarcoding and conventional pelagic netting to inform biodiversity monitoring in deep ocean environments DOI Creative Commons
David Côté, M B McClenaghan, Jessica E. Desforges

и другие.

ICES Journal of Marine Science, Год журнала: 2023, Номер 80(10), С. 2545 - 2562

Опубликована: Ноя. 9, 2023

Abstract The performance of environmental DNA (eDNA) metabarcoding has rarely been evaluated against conventional sampling methods in deep ocean mesopelagic environments. We assessed the biodiversity patterns generated with eDNA and two co-located methods, oblique midwater trawls vertical multinets, to compare regional sample-level diversity. then concordance ecological across water column habitats how markers level effort influenced inferred community. found characterized diversity well, detecting more taxa while identifying similar as samples. Within locations, detected than one replicate. While were within sample stations, fewer compared multinets. Our simulations show greater would improve methods. also observed that using taxonomic data from multiple most those Patterns Exact Sequence Variants stable suggesting they are powerful for change. is a valuable tool monitoring biological hotspots but some methodological adjustments recommended

Язык: Английский

Процитировано

13
North Atlantic deep-sea benthic biodiversity unveiled through sponge natural sampler DNA DOI Creative Commons
Ramón Gallego, María Belén Arias, Andrea Corral‐Lou

и другие.

Communications Biology, Год журнала: 2024, Номер 7(1)

Опубликована: Авг. 19, 2024

The deep-sea remains the biggest challenge to biodiversity exploration, and anthropogenic disturbances extend well into this realm, calling for urgent management strategies. One of most diverse, productive, vulnerable ecosystems in deep sea are sponge grounds. Currently, environmental DNA (eDNA) metabarcoding is revolutionising field monitoring, yet complex benthic remain challenging assess even with these novel technologies. Here, we evaluate effectiveness whole-community characterise metazoan diversity grounds across North Atlantic by leveraging natural eDNA sampling properties sponges themselves. We sampled 97 tissues from four species North-Atlantic biogeographic regions screened them using universal COI barcode region. recovered unprecedented levels taxonomic per unit effort, especially phyla Chordata, Cnidaria, Echinodermata Porifera, at least 406 found our study area. These assemblages identify strong spatial patterns relation both latitude depth, detect emblematic currently employed as indicators habitats. remarkable performance approach different sponges, whole animal kingdom, illustrates vast potential samplers high-resolution biomonitoring solutions highly diverse ecosystems.

Язык: Английский

Процитировано

3
Distribution and habitat preference of polar cod (Boreogadus saida) in the Bering and Chukchi Seas inferred from species-specific detection of environmental DNA DOI Creative Commons
Tatsuya Kawakami, Aya Yamazaki,

Hai-Chao Jiang

и другие.

Frontiers in Marine Science, Год журнала: 2023, Номер 10

Опубликована: Авг. 30, 2023

Ongoing warming and sea-ice reductions in the Arctic can seriously impact cold-water species, such as polar cod ( Boreogadus saida ), necessitating biomonitoring to reveal ecological consequences. Recent methodological advancements environmental DNA (eDNA) techniques have increased our ability conduct monitoring at various locations, including Arctic. This study aimed provide an overview of distribution across Bering Chukchi Seas by employing species-specific detection eDNA. First, we successfully developed novel qPCR assay targeting mitochondrial D-loop region, which exclusively amplifies eDNA derived from cod. Subsequently, was detected using samples that collected latitudinally area during open-water season. Polar primarily surface water central Sea shelf northernmost observation line (75°N), located on slope, off Point Barrow, marginal ice zone. In contrast, only trace amounts were Sea. pattern corresponded well with masses classified based conditions. The clearly limited cold (-1 5°C) low salinity (25–32) water, whereas it a higher range (32–34) middle bottom layers. These findings are consistent current knowledge about habitat cod, suggesting be regarded reliable tool replace or supplement conventional methods. Incorporating into large-scale oceanographic surveys improve spatial temporal resolution fish species reasonable sampling effort will facilitate continuous ecosystems.

Язык: Английский

Процитировано

6
Field collections and environmental DNA surveys reveal topographic complexity of coral reefs as a predictor of cryptobenthic biodiversity across small spatial scales DOI
Ole Brodnicke, Mads Reinholdt Jensen, Philip Francis Thomsen

и другие.

Environmental DNA, Год журнала: 2024, Номер 6(3)

Опубликована: Май 1, 2024

Abstract Coral reefs represent some of the most biodiverse ecosystems in world but are currently undergoing large‐scale degradation due to anthropogenic stressors. Such usually begins with coral bleaching, and if stress condition is inflicted for too long may eventually result loss structural complexity (or “flattening”) reef, dramatically changing habitat availability reef‐associated fauna. Despite having been linked important ecosystem functions, cryptobenthic organisms often overlooked ecological monitoring programs, their microhabitat dependencies poorly understood. Here, we combined collection‐based biodiversity techniques five different environmental DNA (eDNA) sampling (Reef water, sediment, crevice slurp gun, bulk sediment) survey fishes annelids on a Maldivian fringing reef. Collectively, 176 fish 140 annelid taxa were detected eDNA across 14 surveyed sites, more than doubling reported region 88 new occurrences. Water filtered near reef structure revealed highest species richness out tested. Furthermore, found correlations between topographic both collection‐ eDNA‐based techniques. This suggests that detection by be site‐specific predictors reveal community differences small spatial scales (tens meters). We also report flattening (going from complex less sites) can cause 50% reduction diversity was highly associated branching corals. In contrast, communities showed no clear predictors, co‐amplification non‐target, non‐annelid have distorted such present. suggest predictive powers gradients dependent targeted taxa.

Язык: Английский

Процитировано

2
Validation of pop-up satellite archival tags (PSATs) on Atlantic cod (Gadus morhua) in a Greenland fjord DOI Creative Commons
Julius Nielsen, D.A. García Estévez, Søren Post

и другие.

Fisheries Research, Год журнала: 2023, Номер 266, С. 106782 - 106782

Опубликована: Июнь 28, 2023

Traditional tagging techniques are simple and cost-effective, but inferences require recaptures data on movement/migration limited to a start end position at unpredictable intervals. Pop-up satellite archival tags (PSATs) offer other opportunities, as they provide positions pre-programmed times collect on-route data, which can be used describe position, behavior, habitat preferences. Species suitability should, however, documented prior large-scale studies using PSATs. We deployed PSATs six relatively large (total length 84–125 cm) Atlantic cod (Gadus morhua) in inshore West Greenland waters. Three were physically recovered, providing high-resolution depth temperature (readings every 3 s), while three did not report (recovery rate = 50 %). To evaluate the tag's applicability cod, we made detailed behavioral analysis by defining swimming occupied water types phases, cross-evaluated relation depth, stratification identify patterns. Distinct shared patterns behavior evident found no signs of impaired except for an adaptation period lasting up 39 h after release. Generally, pelagic preferred waters ranging 2–5 °C. When encountering colder masses these avoided. During late summer/early autumn, increased vertical activity could some cases linked darkness high-activity event possible predator avoidance. All combined, conclude that suitable monitor natural specimens periods least four months.

Язык: Английский

Процитировано

2
BirT: a novel primer pair for avian environmental DNA metabarcoding DOI Creative Commons
Bettina Thalinger,

R Empey,

Matthew C. Cowperthwaite

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Авг. 9, 2023

Abstract Environmental DNA metabarcoding has become a widely used technique to detect animals from environmental samples and is on the brink of being implemented into routine species monitoring. Surprisingly, birds are among taxonomic groups which until recently, received comparably little attention in eDNA research, fact that changing rapidly, with growing number air analyses. Since hardly ever most abundant aquatic or terrestrial habitats, high specificity employed primers key limit non-target amplifications enable reliable detection samples. Here, we present novel primer pair (BirT) for avian DNA. We optimized fragment length regarding resolution available sequencing technology. Additionally, evaluated availability 12S reference sequences filled database gaps by generating barcodes. Finally, tested applicability this approach using field-collected obtained three different filter types. These results were compared visual observations uploaded eBird ( www.eBird.org ) during sampling period. Our confirm suitability BirT bird length, no amplification groups, provided amplified fragment. Albeit there still substantial sequence database, analysis water resulted species-level 92% detected taxa. All filter/filtration combinations delivered similar total read numbers per sample (mean: 613,972 ± 340,088 SD) 5.5 2.3 SD). Ninety-five percent detections highly plausible 58% confirmed observations. The majority was closely associated habitats confirming waterfowl inhabiting ecological niches via eDNA.

Язык: Английский

Процитировано

2
Environmental DNA metabarcoding reveals seasonal and spatial variation in the vertebrate fauna of Ilulissat Icefjord, Greenland DOI Creative Commons
Sascha Schiøtt, Mads Reinholdt Jensen,

EE Sigsgaard

и другие.

Marine Ecology Progress Series, Год журнала: 2023, Номер 706, С. 91 - 108

Опубликована: Янв. 24, 2023

Ilulissat Icefjord in Greenland is experiencing the effects of climate change, with Sermeq Kujalleq glacier being one fastest-moving and most productive ice streams Greenland. This likely affecting distribution species fjord, including those important to local fisheries. Due heavy conditions, few studies on environmental ecological conditions exist from fjord. However, new techniques such as DNA (eDNA) metabarcoding now allow deeper insight into fjord system. Here, we combine knowledge data hydrographic stable isotopes (δ 18 O), eDNA investigate spatial seasonal marine fish mammals inside Icefjord. Our results support observations that Arctic char migrate southern during summer, harp seals forage large herds system, polar cod dominant prey area, shark does not reside Lower predation pressure Icefjord, due absence bears well limited fishing/hunting, presumably reasons why ringed halibut are larger Furthermore, our indicate branch system has a more diverse community vertebrates different water masses than northern main indicating time lag between inflows branches approach highlights value combining scientific research represents potential starting point for monitoring biological responses associated climate-induced changes.

Язык: Английский

Процитировано

1
Fine-scale oceanographic processes shape marine biodiversity patterns in the Galápagos Islands DOI Creative Commons
Luke E. Holman, Diana A. Pazmiño, Shyam Gopalakrishnan

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 7, 2024

Abstract Uncovering the drivers that shape biodiversity patterns is critical to understand ecological and evolutionary dynamics. Despite evidence composition influenced by processes at different spatial scales, little known about role of fine-scale oceanographic on structure marine communities. This particularly important in hotspot regions, where small changes environmental conditions may lead substantial species composition. We combined ocean modelling 12S DNA (eDNA) metabarcoding, targeting teleost elasmobranch species, explore if biogeographic around biodiverse Galápagos Islands. first detected significant differences eDNA-measured community across archipelago’s diverse seascape. found no relationship between Lagrangian particle tracking metrics nektonic biodiversity, thus developed a novel metric measure cumulative seawater flow resistance pairs geographic sites. explained proportion variation beta dissimilarity sites, comparable influence abiotic drivers, such as temperature distance Cumulatively, our results indicate communities are sensitive local current systems, suggest have an underappreciated structuring globally.

Язык: Английский

Процитировано

0
The Sinking Dead—Arctic Deep‐Sea Scavengers' Diet Suggests Nekton as Vector in Benthopelagic Coupling DOI Creative Commons
Lara Schmittmann, Sophie V. Schindler, Till Bayer

и другие.

Environmental DNA, Год журнала: 2024, Номер 6(5)

Опубликована: Сен. 1, 2024

ABSTRACT Many benthic deep‐sea animals rely on carcasses from the overlying water column that sink to seafloor and form local organic enrichments known as food falls. This flux of carbon shallow pelagic deep sea is part biological pump (BCP) such contributes sequestration. For a complete understanding budgets, it crucial identify diversity distribution sinking which are difficult detect by observational methods. Here, we analyzed diet abundant amphipod scavenger, Eurythenes gryllus , DNA metabarcoding assess their potential falls in Fram Strait, gateway Arctic. E. scavenges nekton but so far was not certain whether this represents main diet. We detected dietary taxa (26 total) 20 out 101 amphipods. found amphipods primarily fed larger including fish, cephalopods, mammals, with bony fish being most targeted source terms abundance. Only one had gelatinous organism. These results support hypothesis targets mostly The differed between Eastern Western suggests regional variability availability. also detected, for first time infections parasitic dinoflagellate Hematodinium . detection demonstrates revealing both web dynamics host–parasite interactions sea. seems promising “natural sampler” monitor will help investigate importance medium‐sized vertical export rapidly changing Arctic Ocean.

Язык: Английский

Процитировано

0