In vitro osteoclast differentiation enhanced by hepatocyte supernatants from high-fat diet mice

Biochemistry and Biophysics Reports, Год журнала: 2024, Номер 39, С. 101788 - 101788

Опубликована: Июль 13, 2024

Язык: Английский

Liver fibrosis is associated with impaired bone mineralization and microstructure in obese individuals with non-alcoholic fatty liver disease.

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Сен. 14, 2022

Abstract Background and purpose Chronic liver diseases are associated with increased bone fracture risk, mostly in end-stage disease cirrhosis; besides, data non-alcoholic fatty (NAFLD) limited. Aims of this study was to investigate mineralization microstructure obese individuals NAFLD relation the estimated fibrosis. Methods We analyzed from 1872 (44.6 ± 14.1 years, M/F: 389/1483; BMI: 38.3 5.3 kg/m 2 ) referring Endocrinology outpatient clinics Sapienza University, Rome, Italy. Participants underwent clinical work-up, Dual-Energy X-ray Absorptiometry for assessing mineral density (BMD) microarchitecture (trabecular score, TBS). Liver fibrosis by Fibrosis Score 4 (FIB-4). Serum parathyroid hormone (PTH), 25(OH) vitamin D, osteocalcin IGF-1 levels were measured. Results Individuals osteopenia/osteoporosis had greater FIB-4 than those normal BMD (p < 0.001). progressively presence degraded 0.001) negatively correlated serum 0.001), which both reduced osteopenia/osteoporosis. predicted reduction multivariable regression models adjusted confounders (β: −0.18, p Higher fragility OR 3.8 (95%C.I:1.5–9.3); association persisted significant after adjustment sex, age, BMI, diabetes, smoking status PTH at logistic analysis (OR 1.91 (95%C.I:1.15–3.17), 0.01), AUROC = 0.842 (95%C.I:0.795–0.890; Conclusion Our indicate a tight between NAFLD-related fibrosis, lower individuals, suggesting potential common pathways underlying involvement insulin-resistance disorders.

Язык: Английский

Osteoprotegerin deficiency aggravates methionine- choline- deficient diet-induced nonalcoholic steatohepatitis in mice

Research Square (Research Square), Год журнала: 2022, Номер unknown

Опубликована: Ноя. 18, 2022

Abstract Background & Aims: Clinical studies have shown that osteoprotegerin (OPG) is reduced in patients with nonalcoholic steatohepatitis (NASH), but the underlying mechanisms are unclear. The current study focuses on role of OPG NASH pathogenesis. Methods knockout mice and wild-type control fed a methionine choline-deficient diet (MCD) for 4 weeks resulted an animal model NASH. Measurement triglycerides (TG) serum liver to assess steatosis. Hematoxylin eosin (HE), Sirius Red Masson staining were used damage. Transcriptome sequencing analysis, qPCR western blot analyze changes lipid metabolism inflammation-related indicators liver. Results In vivo reduction TG levels significant increase ALT AST. expression inflammatory factors fibrosis genes was significantly upregulated livers mice. analysis showed enhanced MCD diet-induced activation mitogen-activated protein kinase (MAPK) signaling pathway. Mechanistically, may inhibit MAPK pathway activity by upregulating dual specificity phosphatase 14 (DUSP14), thereby reducing injury. Conclusion be drug target treatment

Язык: Английский