Biochar Co-Applied with Lime Enhances Soil Phosphorus Availability via Microbial and Enzymatic Modulation of Paddy Soil

Microorganisms, Год журнала: 2025, Номер 13(3), С. 582 - 582

Опубликована: Март 4, 2025

Soil microorganisms play a crucial role in improving soil phosphorus (P) availability. However, few studies have explored the changes microbial community structure and their underlying mechanisms for P availability with application of biochar lime. Three kinds biochar, made from rice straw (SB), Chinese fir wood sawdust (WB), pig manure (MB), alone lime (SBL, WBL, MBL), were applied to paddy reveal biochemical enhancing High-throughput sequencing real-time PCR used investigate communities functional genes. The three biochars increased soil’s available order MB > SB WB. Biochar co-applied (Olsen-P by 169–209%) inorganic (Al-P 53.4–161%, Fe-P 96.3–198%, Ca-P 59.0–154%) more than alone, compared control (CK). Both activities alkaline phosphomonoesterase (ALP), phosphodiesterase (PD), pyrophosphatase (IPP) 369–806%, 28.4–67.3%, 37.9–181%, respectively, while it decreased activity acidic (ACP) 15.1–44.0%, CK. Biochar, both lime, reduced copy number phoC gene 5.37–88.7%, phoD, gcd, pqqC genes 51.3–533%, 62.1–275%, 25.2–158%, A correlation analysis partial least squares path modeling (PLS-PM) indicated that Olsen-P, Bray-1 P, significantly positively correlated ALP, PD, IPP, phoD gene. relative abundances phoD-harboring bacteria Proteobacteria, Firmicutes, Acidobacteria, which promoted transformation effective state. Meanwhile, dominant species Anaerolinea, Ascomycota, Mucoromycota, Chaetomium provided rich nutrients accelerating decomposition organic matter, thus promoting phosphatase activity. It could be inferred optimized improved increasing nutrients, had better effect on yields alone.

Язык: Английский

Soil Inorganic Phosphorus Is Closely Associated with pqqC- Gene Abundance and Bacterial Community Richness in Grape Orchards with Different Planting Years

Agronomy, Год журнала: 2025, Номер 15(3), С. 666 - 666

Опубликована: Март 7, 2025

The high application rate and low utilization efficiency of inorganic phosphorus (Pi) fertilizer could lead to significant P accumulation in soil. Soil cycling is greatly affected by the planting time perennial fruit yards. However, mechanism which soil Pi fractions pqqC-harboring bacterial communities, their relationships, are vines, remains unclear. Here, fractions, relationships grape yards with 0.5, 4, 16 22 growth years, designated as Y0.5, Y4, Y16 Y22, were examined. results showed that increasing organic carbon (SOC) contents pH values, respectively, increased decreased. In addition, percentages labile moderately increased, whereas those stable soils abundance α-diversity pqqC decreased compared Y0.5. Y16, composition communities was altered significantly, showing a great difference Y4 Y22. At genus level, relative bacteria highly correlated fractions. Further structural equation modeling revealed between community richness gene transformation regulated pH. These findings suggest changes closely associated pH, abundance, SOC content orchards different years.

Язык: Английский

Functional Characteristics and Cellulose Degradation Genes of the Microbial Community in Soils with Different Initial pH Values

Agriculture, Год журнала: 2025, Номер 15(10), С. 1068 - 1068

Опубликована: Май 15, 2025

Soil pH critically regulates microbial community structure and activity, thereby influencing carbon transformation processes in terrestrial ecosystems. However, the mechanisms underlying pH-mediated shifts metabolic functions cellulose-degrading functional genes remain poorly understood. This study investigated responses of bacterial communities, profiles, abundance cellobiohydrolase I (cbhI) glycoside hydrolase family 48 (GH48) to varying levels fluvo-aquic red soils. High-throughput sequencing, PICRUSt-based prediction, quantitative PCR were employed analyze composition, traits, gene dynamics. Network analysis clarified linkages between genes, pathways, taxa. The results revealed that elevated significantly increased CO2 emissions dissolved organic (DOC) content both Dominant taxa, including Alphaproteobacteria, Bacteroidetes, Xanthomonadaceae, Mycoplasma, exhibited pH-dependent enrichment. Metabolic predictions indicated positively influenced linked biodegradation xenobiotic metabolism soil but suppressed energy-metabolism-related genes. Contrastingly, soil, cbhI GH48 declined with rising pH, suggesting acidic conditions favor cellulolytic activity. identified strong positive correlations Caulobacteraceae, while closely associated taxa such as Comamonadaceae, Micromonosporaceae, which drive matter decomposition. These findings underscore a pivotal regulator expression, soil-specific highlighting need for tailored strategies optimize cycling sequestration agricultural

Язык: Английский