BuZhong YiQi Formula Alleviates Diabetes-Caused Hyposalivation by Activating Salivary Secretion Pathway in the Parotid and Submandibular Glands of Rats DOI Creative Commons
Mingyu Wang,

Z.-Y. Hu,

Liang Wang

и другие.

Pharmaceuticals, Год журнала: 2025, Номер 18(3), С. 377 - 377

Опубликована: Март 6, 2025

Background/Objectives: BuZhong Yiqi Formula (BZYQF) has significant ameliorative effects on type 2 diabetes mellitus (T2DM). However, its efficacy in alleviating the hyposalivation caused by T2DM needs to be confirmed, and mechanism is unclear. Methods: Network pharmacology molecular docking were combined analyze which BZYQF alleviates T2DM-caused hyposalivation. A rat model was induced evaluate of BZYQF. The total saliva before after acid stimulation collected determine salivary flow rate alpha-amylase (sAA) activity. parotid (PG) submandibular glands (SMG) experimental rats removed perform histopathology observation, biochemical indicator determination, expression detection signaling molecules secretion pathway. Results: present study screened out 1014 potential targets regarding treatment T2DM. These mainly involved formation receptor complex, exercising neurotransmitter activity regulating secretion. They significantly enriched pathway β1-AR/PKA/AMY1 CHRM3/IP3R/AQP5. Furthermore, BZYQF, nine validated compounds able dock into active site β1-AR, three CHRM3. Animal experiments confirmed that reduces fasting blood glucose, cholesterol triglyceride levels; enhances insulin level HOMA-IS (p < 0.05); increases (Basal: increase from 21.04 ± 14.31 42.65 8.84 μL/min, effect size Cohen’s d = 6.80, p 0.0078; Stimulated: 36.88 17.48 72.63 17.67 7.61, 0.0025) sAA 0.68 0.32 2.17 0.77 U/mL, 9.49, 0.0027; 1.15 4.80 1.26 13.10, 0.0001) basal stimulated rats. Further mechanistic studies revealed glucose lipid accumulation, acetylcholine content, improves pathological lesions inflammation, molecules, including PKA, IP3R, AQP5, CHRM3, AMY1 PG SMG 0.05). Conclusions: demonstrated alleviate improving metabolism activating This might provide a novel rationale strategy for diabetes-induced clinical setting.

Язык: Английский

BuZhong YiQi Formula Alleviates Diabetes-Caused Hyposalivation by Activating Salivary Secretion Pathway in the Parotid and Submandibular Glands of Rats DOI Creative Commons
Mingyu Wang,

Z.-Y. Hu,

Liang Wang

и другие.

Pharmaceuticals, Год журнала: 2025, Номер 18(3), С. 377 - 377

Опубликована: Март 6, 2025

Background/Objectives: BuZhong Yiqi Formula (BZYQF) has significant ameliorative effects on type 2 diabetes mellitus (T2DM). However, its efficacy in alleviating the hyposalivation caused by T2DM needs to be confirmed, and mechanism is unclear. Methods: Network pharmacology molecular docking were combined analyze which BZYQF alleviates T2DM-caused hyposalivation. A rat model was induced evaluate of BZYQF. The total saliva before after acid stimulation collected determine salivary flow rate alpha-amylase (sAA) activity. parotid (PG) submandibular glands (SMG) experimental rats removed perform histopathology observation, biochemical indicator determination, expression detection signaling molecules secretion pathway. Results: present study screened out 1014 potential targets regarding treatment T2DM. These mainly involved formation receptor complex, exercising neurotransmitter activity regulating secretion. They significantly enriched pathway β1-AR/PKA/AMY1 CHRM3/IP3R/AQP5. Furthermore, BZYQF, nine validated compounds able dock into active site β1-AR, three CHRM3. Animal experiments confirmed that reduces fasting blood glucose, cholesterol triglyceride levels; enhances insulin level HOMA-IS (p < 0.05); increases (Basal: increase from 21.04 ± 14.31 42.65 8.84 μL/min, effect size Cohen’s d = 6.80, p 0.0078; Stimulated: 36.88 17.48 72.63 17.67 7.61, 0.0025) sAA 0.68 0.32 2.17 0.77 U/mL, 9.49, 0.0027; 1.15 4.80 1.26 13.10, 0.0001) basal stimulated rats. Further mechanistic studies revealed glucose lipid accumulation, acetylcholine content, improves pathological lesions inflammation, molecules, including PKA, IP3R, AQP5, CHRM3, AMY1 PG SMG 0.05). Conclusions: demonstrated alleviate improving metabolism activating This might provide a novel rationale strategy for diabetes-induced clinical setting.

Язык: Английский

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