Toward the Development of the Trojan Female Technique in Pest Insects: Male‐Specific Influence of Mitochondrial Haplotype on Reproductive Output in the Seed Beetle Acanthoscelides obtectus DOI Creative Commons

Vlajnić Lea,

Savković Uroš,

Jović Jelena

и другие.

Evolutionary Applications, Год журнала: 2024, Номер 17(12)

Опубликована: Дек. 1, 2024

ABSTRACT Biocontrol techniques that impair reproductive capacity of insect pests provide opportunities to control the dynamics their populations while minimizing collateral damage non‐target species and environment. The Trojan Female Technique, or TFT, is a method trans‐generational fertility‐based population through release females carry mitochondrial DNA mutations negatively affect male, but not female, output. TFT based on evolutionary hypothesis that, due maternal inheritance mitochondria, which are beneficial neutral in harmful males can accumulate genome without selection acting against them. Although has been theoretically substantiated, empirical work date focused only Drosophila melanogaster populations, existence male‐biased approach economically important pest remain unexplored. Here, we examined sex‐specific effects three distinct naturally occurring haplotypes (MG1a, MG1d, MG3b) several life history traits seed beetle Acanthoscelides obtectus . Our results revealed harboring MG3b mitotype exhibited lower early fecundity fertility, there were no longevity either sex. experiments support for variant specifically impairs male output insects. These be harnessed further develop as novel form biocontrol with broad applicability economic disease vector

Язык: Английский

Conservation Mitonuclear Replacement: Facilitated mitochondrial adaptation for a changing world DOI Creative Commons
Erik N. K. Iverson

Evolutionary Applications, Год журнала: 2024, Номер 17(3)

Опубликована: Март 1, 2024

Abstract Most species will not be able to migrate fast enough cope with climate change, nor evolve quickly current levels of genetic variation. Exacerbating the problem are anthropogenic influences on adaptive potential, including prevention gene flow through habitat fragmentation and erosion diversity in small, bottlenecked populations. Facilitated adaptation, or assisted evolution, offers a way augment variation via artificial selection, induced hybridization, engineering. One key source variation, particularly for climatic core metabolic genes encoded by mitochondrial genome. These influence environmental tolerance heat, drought, hypoxia, but must interact intimately co‐evolve suite important nuclear genes. coadapted mitonuclear form some reproductive barriers between species. Mitochondrial genomes can do introgress an manner, they may co‐introgress maintaining compatibility. Managers should consider relevance variability conservation decision‐making, as tool facilitating adaptation. I propose novel technique dubbed Conservation Mitonuclear Replacement (CmNR), which entails replacing machinery threatened species—the genome loci—with those from closely related divergent population, better‐adapted changes carry lower load. The most feasible route CmNR is combine CRISPR‐based editing replacement technologies. This method preserves much organism's phenotype could allow populations persist wild when no other suitable options exist. mountaintops, where rising temperatures threaten alarming number almost certain extinction next century.

Язык: Английский

Процитировано

4

The Complexities of Interspecies Somatic Cell Nuclear Transfer: From Biological and Molecular Insights to Future Perspectives DOI Open Access
Peachanika Pankammoon, Marvin Bryan Segundo Salinas, Chatchote Thitaram

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3310 - 3310

Опубликована: Апрель 2, 2025

For nearly three decades, interspecies somatic cell nuclear transfer (iSCNT) has been explored as a potential tool for cloning, regenerative medicine, and wildlife conservation. However, developmental inefficiencies remain major challenge, largely due to persistent barriers in nucleocytoplasmic transport, mitonuclear communication, epigenome crosstalk. This review synthesized peer-reviewed English articles from PubMed, Web of Science, Scopus, spanning using relevant keywords explore the molecular mechanisms underlying iSCNT improvement strategies. We highlight recent findings deepening understanding iSCNT, emphasizing their interconnected complexities, including following: (1) incompatibility may disrupt pore complex (NPC) assembly maturation, impairing transport essential transcription factors (TFs), embryonic genome activation (EGA), reprogramming; (2) could lead mitochondrial DNA (nDNA-mtDNA) mismatches, affecting electron chain (ETC) assembly, oxidative phosphorylation, energy metabolism; (3) these interrelated incompatibilities can further influence epigenetic regulation, potentially leading incomplete reprogramming embryos. Addressing challenges requires multifaceted, species-specific approach that balances multiple rather than isolating single factor. Gaining insight into interactions between donor nucleus recipient cytoplast, coupled with optimizing strategies tailored specific pairings, significantly enhance efficiency, ultimately transforming experimental breakthroughs real-world applications reproductive biotechnology, species

Язык: Английский

Процитировано

0

An evolving roadmap: using mitochondrial physiology to help guide conservation efforts DOI Creative Commons
Elisa Thoral, Neal J. Dawson, Stefano Bettinazzi

и другие.

Conservation Physiology, Год журнала: 2024, Номер 12(1)

Опубликована: Янв. 1, 2024

Abstract The crucial role of aerobic energy production in sustaining eukaryotic life positions mitochondrial processes as key determinants an animal's ability to withstand unpredictable environments. advent new techniques facilitating the measurement function offers increasingly promising tool for conservation approaches. Herein, we synthesize current knowledge on links between bioenergetics, ecophysiology and local adaptation, expanding them wider physiology field. We discuss recent findings linking cellular bioenergetics whole-animal fitness, context climate change. summarize topics, questions, methods, pitfalls caveats help provide a comprehensive roadmap studying mitochondria from perspective. Our overall aim is guide natural populations, outlining methods that could be most useful assess

Язык: Английский

Процитировано

1

Toward the Development of the Trojan Female Technique in Pest Insects: Male‐Specific Influence of Mitochondrial Haplotype on Reproductive Output in the Seed Beetle Acanthoscelides obtectus DOI Creative Commons

Vlajnić Lea,

Savković Uroš,

Jović Jelena

и другие.

Evolutionary Applications, Год журнала: 2024, Номер 17(12)

Опубликована: Дек. 1, 2024

ABSTRACT Biocontrol techniques that impair reproductive capacity of insect pests provide opportunities to control the dynamics their populations while minimizing collateral damage non‐target species and environment. The Trojan Female Technique, or TFT, is a method trans‐generational fertility‐based population through release females carry mitochondrial DNA mutations negatively affect male, but not female, output. TFT based on evolutionary hypothesis that, due maternal inheritance mitochondria, which are beneficial neutral in harmful males can accumulate genome without selection acting against them. Although has been theoretically substantiated, empirical work date focused only Drosophila melanogaster populations, existence male‐biased approach economically important pest remain unexplored. Here, we examined sex‐specific effects three distinct naturally occurring haplotypes (MG1a, MG1d, MG3b) several life history traits seed beetle Acanthoscelides obtectus . Our results revealed harboring MG3b mitotype exhibited lower early fecundity fertility, there were no longevity either sex. experiments support for variant specifically impairs male output insects. These be harnessed further develop as novel form biocontrol with broad applicability economic disease vector

Язык: Английский

Процитировано

1