StreptomycesVolatile Compounds Influence Exploration and Microbial Community Dynamics by Altering Iron Availability

mBio, Год журнала: 2019, Номер 10(2)

Опубликована: Март 4, 2019

Microbial growth and community interactions are influenced by a multitude of factors. A new mode Streptomyces growth—exploration—is promoted with the yeast Saccharomyces cerevisiae requires emission trimethylamine (TMA), pH-raising volatile compound. We show here that TMA also profoundly alters environment around exploring cultures. It specifically reduces iron availability, this in turn adversely affects viability surrounding microbes. Paradoxically, bacteria thrive these iron-depleted niches, both rewiring their gene expression metabolism to facilitate uptake increasing exploration rate. Growth close proximity other microbes adept at enhances exploration. Collectively, data from work reveal role for bacterial compounds modulating nutrient availability microbial behavior. The results further expand repertoire interspecies cues impact provide mechanistic insight into unique growth.

Язык: Английский

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Siderophore-Mediated Interactions Determine the Disease Suppressiveness of Microbial Consortia

mSystems, Год журнала: 2020, Номер 5(3)

Опубликована: Июнь 29, 2020

Interactions between plant pathogens and root-associated microbes play an important role in determining disease outcomes. While several studies have suggested that steering these interactions may improve health, such approaches remained challenging practice. Because of low iron availability most soils, competition for via secreted siderophore molecules might influence microbial interaction Here, we tested if bacterial mediated by iron-scavenging siderophores can be used to predict the suppressiveness consortia against soilborne Ralstonia solanacearum, a pathogen tomato rhizosphere. Iron significantly affected within inoculated pathogen. We observed contrasting effects other nonsiderophore metabolites on growth, while were relatively much stronger. Specifically, incidence was reduced vivo when produced could not use its own growth. Employing siderophore-mediated engineer functionally robust inoculants shows promise protecting plants from pathogens.IMPORTANCE Soil-borne cause high losses crop yields globally. The development environmentally friendly is urgently needed, but often constrained complex pathogens. demonstrate reducing infection enhancing health. This study provides promising novel research direction dealing with wide range infections through exploitation, which colonization both human hosts

Язык: Английский

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Siderophores drive invasion dynamics in bacterial communities through their dual role as public good versus public bad

Ecology Letters, Год журнала: 2021, Номер 25(1), С. 138 - 150

Опубликована: Ноя. 9, 2021

Microbial invasions can compromise ecosystem services and spur dysbiosis disease in hosts. Nevertheless, the mechanisms determining invasion outcomes often remain unclear. Here, we examine role of iron-scavenging siderophores driving Pseudomonas aeruginosa into resident communities environmental pseudomonads. Siderophores be 'public goods' by delivering iron to individuals possessing matching receptors; but they also bads' withholding from competitors lacking these receptors. Accordingly, should either promote or impede invasion, depending on their effects invader growth. Using supernatant feeding assays, show that success indeed increased when could use its inhibit (public bad) rather than stimulate good) Conversely, decreased more was inhibited residents' siderophores. Our findings identify as a major driver dynamics bacterial under iron-limited conditions.

Язык: Английский

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Kin selection explains the evolution of cooperation in the gut microbiota

Proceedings of the National Academy of Sciences, Год журнала: 2021, Номер 118(6)

Опубликована: Фев. 1, 2021

Significance This is a comparative study attempting to explain the pattern of cooperation across number microbial species. Hamilton’s inclusive-fitness theory makes very general prediction that increased genetic relatedness should drive evolution cooperation. Various arguments have dismissed validity this in microbes, but without ever testing broad taxonomic support for those arguments. Here, we rehabilitate central role by showing its power predict cooperative gene content holds full diversity human gut microbiota. Explaining broad-scale patterns critical unifying variable predictive science and applications. The manipulation may offer an opportunity engineering communities, such as

Язык: Английский

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Bacillus velezensis strain Ag75 as a new multifunctional agent for biocontrol, phosphate solubilization and growth promotion in maize and soybean crops

Scientific Reports, Год журнала: 2022, Номер 12(1)

Опубликована: Сен. 10, 2022

Abstract Soybean and maize are some of the main drivers Brazilian agribusiness. However, biotic abiotic factors great concern, causing huge grain yield quality losses. Phosphorus (P) deficiency is important among because most soils have a highly P-fixing nature. Thus, large amounts phosphate fertilizers regularly applied to overcome rapid precipitation P. Searching for alternatives improve use P by crops essential reduce demand input. The multifunctional rhizobacteria can be considered one these alternatives. In this sense, objective present work was select validate bacterial strains with triple action (plant growth promoter, solubilizer, biocontrol agent) in soybean, aiming develop microbial inoculant agriculture. Bacterial high indole acetic acid (IAA) production, solubilization, antifungal activity against soil pathogenic fungi ( Rhizoctonia solani , Macrophomina phaseolina Fusarium ) were selected from rhizosphere. Then, they evaluated as promoters under greenhouse conditions. Based on study, strain 03 (Ag75) due its potential increasing biomass (root shoot) shoot content maize. This identified through genomic sequencing Bacillus velezensis . field experiments, inoculation bacterium increased soybean yields 17.8 26.5%, respectively, compared control (25 kg 2 O 5 ). addition, results did not differ 84 indicating that it possible application crops. Ag75 has developing combines ability solubilize phosphate, promote plant growth, agent several phytopathogenic fungi.

Язык: Английский

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