Genes,
Год журнала:
2023,
Номер
14(8), С. 1648 - 1648
Опубликована: Авг. 18, 2023
(1)
Background:
It
was
found
that
the
melanin
of
black-bone
chicken
has
various
effects
such
as
scavenging
DPPH
free
radicals
and
anti-oxidation,
synthesis
is
affected
by
factors
including
hormones.
In
addition,
several
studies
have
melatonin
affects
melanoma
cell
melanin,
which
not
been
reported
in
primary
melanocytes;
so,
relevant
were
conducted.
(2)
Methods:
this
study,
melanocytes
isolated
characterized,
then
treated
with
different
concentrations
to
investigate
on
terms
synthesis-related
genes,
content,
tyrosinase
activity,
combined
RNA
seq
detect
change
gene
expression
level
after
treatment.
(3)
Results:
We
characterized
melanocytes,
indirect
immunofluorescence
assay
results
showed
positive
melanocyte
marker
genes.
RT-qPCR
decreased
reduced
content
activity
group.
A
total
1703
differentially
expressed
genes
screened
RNA-seq,
KEGG
results,
signaling
pathway
associated
synthesis,
mTOR
enriched.
(4)
Conclusions:
Melatonin
could
decrease
melanocytes.
Frontiers in Physiology,
Год журнала:
2025,
Номер
15
Опубликована: Янв. 9, 2025
Recent
findings
show
that
visible
light,
particularly
blue
stimulates
melanogenesis
in
human
skin,
though
the
underlying
mechanisms
remain
debated.
This
study
aimed
to
determine
cell
damage
threshold
of
non-ionizing
light
on
keratinocytes
while
preserving
their
ability
stimulate
melanogenesis.
Human
(N
=
3)
and
melanocytes
were
isolated
from
skin
samples
varying
Fitzpatrick
phototypes
irradiated
with
(λpeak
457
nm)
UVA
385
nm).
Cellular
metabolic
activity
was
assessed
using
AlamarBlue
HS
assay,
α-Melanocyte-Stimulating
Hormone
(α-MSH)
production
by
quantified
ELISA,
Western
blotting
used
assess
pro-melanogenic
factor
expression
melanocytes.
High
intensity
(50
mW/cm2,
50
J/cm2)
(15
20
significantly
reduced
cellular
activity,
a
0.86
±
0.055
0.60
0.031
(mean
SD)
fold
decrease
compared
respective
sham
day
7.
In
contrast,
moderate
intensities
(5-15
10-20
preserved
stimulating
α-MSH
production,
an
optimal
balance
achieved
at
10
15
J/cm2
(1.14
0.046
increase
relative
7).
Co-culture
experiments
confirmed
enhanced
via
paracrine
signaling,
increasing
Tyrosinase
Dopachrome
Tautomerase
(DCT).
Direct
irradiation
also
increased
pigmentation
without
significant
damage.
Moderate-intensity
effectively
maintaining
both
melanocytes,
offering
promising,
safe
approach
for
therapies
targeting
disorders.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(21), С. 15963 - 15963
Опубликована: Ноя. 4, 2023
Human
skin
aging
is
associated
with
functional
deterioration
on
multiple
levels
of
physiology,
necessitating
the
development
effective
senotherapeutics.
The
well-tolerated
neurohormone
melatonin
unfolds
anti-aging
properties
in
vitro
and
vivo,
but
it
remains
unclear
whether
these
effects
translate
to
aged
human
ex
vivo.
We
tested
this
organ-cultured,
full-thickness
eyelid
(5-6
donors;
49-77
years)
by
adding
culture
medium,
followed
assessment
core
biomarkers
via
quantitative
immunohistochemistry.
Over
6
days,
200
µM
significantly
downregulated
intraepidermal
activity
aging-promoting
mTORC1
pathway
(as
visualized
reduced
S6
phosphorylation)
MMP-1
protein
expression
epidermis
compared
vehicle-treated
control
skin.
Conversely,
transmembrane
collagen
17A1,
a
key
stem
cell
niche
matrix
molecule
that
declines
aging,
mitochondrial
markers
(e.g.,
TFAM,
MTCO-1,
VDAC/porin)
were
upregulated.
Interestingly,
100
also
increased
epidermal
VEGF-A
protein,
which
required
sufficient
for
inducing
rejuvenation.
In
dermis,
fibrillin-1
improved
fibrillin
structural
organization,
indicating
an
elastic
fiber
network.
contrast,
other
(SIRT-1,
lamin-B1,
p16INK4,
I)
remained
unchanged.
This
vivo
study
provides
proof
principle
indeed
exerts
long-suspected
never
conclusively
demonstrated
surprisingly
differential
dermis.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(5), С. 4780 - 4780
Опубликована: Март 1, 2023
Quercetin
3-O-(6″-O-E-caffeoyl)-β-D-glucopyranoside
is
a
flavonoid
compound
produced
by
various
plants
with
reported
antiprotozoal
potential
against
E.
histolytica
and
G.
lamblia;
however,
its
effects
on
skin
pigment
regulation
have
not
been
studied
in
detail.
In
this
investigation,
we
discovered
that
quercetin
3-O-(6″-O-E-caffeoyl)-D-glucopyranoside
(coded
as
CC7)
demonstrated
more
increased
melanogenesis
effect
B16
cells.
CC7
exhibited
no
cytotoxicity
or
effective
stimulating
melanin
content
intracellular
tyrosinase
activity.
This
melanogenic-promoting
was
accompanied
activated
expression
levels
of
microphthalmia-associated
transcription
factor
(MITF),
key
melanogenic
regulatory
factor,
enzymes,
(TYR)
tyrosinase-related
protein-1
(TRP-1)
2
(TRP-2)
the
CC7-treated
Mechanistically,
found
exerted
upregulating
phosphorylation
stress-regulated
protein
kinase
(p38)
c-Jun
N-terminal
(JNK).
Moreover,
upregulation
phosphor-protein
B
(Akt)
Glycogen
synthase
kinase-3
beta
(GSK-3β)
β-catenin
cell
cytoplasm,
subsequently,
it
translocated
into
nucleus,
resulting
melanogenesis.
Specific
inhibitors
P38,
JNK,
Akt
validated
promotes
synthesis
activity
regulating
GSK3β/β-catenin
signaling
pathways.
Our
results
support
involves
MAPKs
Akt/GSK3β/β-catenin
Endocrine Reviews,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 30, 2024
Skin
is
the
largest
organ
of
human
body
and
undergoes
both
intrinsic
(chronological)
extrinsic
aging.
While
skin
aging
driven
by
genetic
epigenetic
factors,
mediated
external
threats
such
as
UV
irradiation
or
fine
particular
matters,
sum
which
referred
to
exposome.
The
clinical
manifestations
biochemical
changes
are
different
between
aging,
albeit
overlapping
features
exist,
eg,
increased
generation
reactive
oxygen
species,
extracellular
matrix
degradation,
telomere
shortening,
lipid
peroxidation,
DNA
damage.
As
a
prominent
target
for
many
hormones,
molecular
processes
underlying
under
tight
control
classical
neuroendocrine
axes.
However,
also
an
endocrine
itself,
including
hair
follicle,
fully
functional
"miniorgan."
Here
we
review
pivotal
hormones
controlling
focusing
on
IGF-1,
key
fibroblast-derived
orchestrator
GH,
estrogens,
retinoids,
melatonin.
emerging
roles
additional
players,
ie,
α-melanocyte-stimulating
hormone,
central
player
hypothalamic-pituitary-adrenal
axis;
members
hypothalamic-pituitary-thyroid
oxytocin,
endocannabinoids,
peroxisome
proliferator-activated
receptor
modulators,
reviewed.
Until
now,
only
limited
number
these
mainly
topical
retinoids
have
found
their
way
into
practice
anti-skin
compounds.
Further
research
biological
properties
players
its
derivatives
may
offer
development
novel
senotherapeutics
treatment
prevention
British Journal of Dermatology,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 9, 2024
Topical
melatonin
applied
over
many
days
and
weeks,
which
might
even
reduce
dermatitis,
erythema
sunburn,
could
be
a
promising
start
in
the
lifelong
battle
to
impede
and,
ideally,
reverse
intrinsic
extrinsic
human
skin
ageing.
