Sulfated Glycosaminoglycans as Inhibitors for Chlamydia Infections: Molecular Weight and Sulfation Dependence

Macromolecular Bioscience, Год журнала: 2025, Номер unknown

Опубликована: Янв. 21, 2025

Abstract Glycosaminoglycans (GAGs) play a pivotal role in pathogen attachment and entry into host cells, where the interaction with GAGs is critical for diverse range of bacteria viruses. This study focuses on elucidating specific interactions between sulfated adhesin OmcB (Outer membrane complex protein B) Chlamydia species, examining how structural characteristics GAGs, such as sulfation degree molecular weight, influence their binding affinity thereby affect bacterial infectivity. A surface‐based assay established to determine constants various GAGs. It shown that increased higher weight enhance GAG OmcB. These findings are further validated using cell assays, which shows addition reduces OmcB‐cell inhibits C. pneumoniae elementary bodies (EBs), underscoring chlamydial infections. Notably, heparin exhibites stronger inhibitory effect compare similar degrees weights, suggesting particular architectures may optimize interactions.

Язык: Английский

---

Proteoglycan-degrading enzymes engineered for enhanced tumor microenvironment interaction in renal cell carcinoma

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140440 - 140440

Опубликована: Янв. 1, 2025

Язык: Английский

---

Therapeutic targets in aging-related osteoarthritis: A focus on the extracellular matrix homeostasis

Life Sciences, Год журнала: 2025, Номер unknown, С. 123487 - 123487

Опубликована: Фев. 1, 2025

Язык: Английский

---

Cylindracin, a Fruiting Body-Specific Protein of Cyclocybe cylindracea, Represses the Egg-Laying and Development of Caenorhabditis elegans and Drosophila melanogaster

Toxins, Год журнала: 2025, Номер 17(3), С. 118 - 118

Опубликована: Март 1, 2025

Mushrooms are a valuable source of bioactive compounds to develop efficient, secure medicines and environmentally friendly agrochemicals. Cylindracin is small cysteine-rich protein that specifically expressed in the immature fruiting body edible mushroom Cyclocybe cylindracea. Recombinant (rCYL), comprising C-terminal domain cylindracin, inhibits hyphal growth conidiogenesis filamentous fungi. Here, we show rCYL represses egg-laying development Caenorhabditis elegans Drosophila melanogaster. The feeding at 16 µM reduced volume C. larvae approximately 60% when compared control. At same concentration, repressed frequencies pupation emergence D. melanogaster 74% 40%, respectively, In virgin adult flies, 47 substantially frequency egg-laying, next generation, especially for females. These inhibitory effects gradually disappeared after ceasing ingestion rCYL. use fluorescence-labeled revealed accumulates pharynx cuticles elegans. melanogaster, was detected primarily midguts lesser degree hindguts, ovaries, testes, malpighian tubules. stable against trypsin, chymotrypsin, pepsin, whereas it did not inhibit proteolytic glycolytic enzymes vitro. oligomerized formed amyloid-like aggregates through binding heparin heparan sulfate results suggest has potential as new biocontrol agent pests.

Язык: Английский

---

The interplay between endothelial glycocalyx maturity and both the toxicity and intracellular uptake of charged nanoparticles

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

---

Integrated Single‐Cell Transcriptome and eQTL Analyses Reveal the Role of PZP in Aging and Chronic Kidney Disease

The Journal of Gene Medicine, Год журнала: 2025, Номер 27(3)

Опубликована: Март 1, 2025

Aging is a known driver of chronic kidney disease (CKD), yet the genetic mechanisms linking these two conditions remain unclear. This study aims to explore role CD8+ central memory T (TCM) cells and their associated gene expression in interaction between aging CKD. Peripheral blood samples from young controls, elderly individuals, CKD patients were analyzed using single-cell RNA sequencing investigate immune cell populations. Expression quantitative trait loci (eQTL) Mendelian randomization analyses performed data genomic cohorts, including UK Biobank FinnGen, assess causal relationships. Experimental validation evaluated correlations pregnancy zone protein (PZP) clinical indicators such as age, glomerular filtration rate (GFR), serum creatinine, proteinuria. Increased proportions TCM observed individuals patients. PZP was identified key factor progression aging, linked metabolic reprogramming impaired intercellular communication. correlated significantly with (r = 0.818, p 0.047), reduced GFR -0.557, 0.011), elevated creatinine 0.507, 0.019), proteinuria 0.761, 0.047). Shared immunological link contributing dysregulation inflammation. The dual PZP, involving its upregulation, disrupted communication, reprogramming, highlights potential biomarker therapeutic target for aging-associated diseases.

Язык: Английский

---

Nanoparticles and Glycosaminoglycans: Progress and Applications for Cancer Therapy

Proteoglycan Research, Год журнала: 2025, Номер 3(1)

Опубликована: Янв. 1, 2025

ABSTRACT Glycosaminoglycans (GAGs) play essential roles in biological processes such as cell signaling and extracellular matrix assembly. Alterations the structure of GAGs have been implicated cancer pathogenesis, demonstrating their potential biomarkers for diagnosis progression, well targets pharmacological interventions. For these reasons, attracting considerable interest applications therapies. On one side, are optimal targeting sites drug delivery systems enhancing specificity, but on other interactions can also alter function cellular uptake therapeutics. This is particularly true nanoscaled review aims at highlighting role regulating nanoparticles (NPs) exploitation NP–GAG effective delivery, cells. We will include relevant examples GAG‐functionalized NPs targeted imaging, theranostic applications, showing that GAG stabilization prevent agglomeration improve functionality.

Язык: Английский

---

Changes in musculoskeletal adaptation to physical activity in connective tissue dysplasia: analyzing some triggering factors

Sports medicine research and practice, Год журнала: 2025, Номер 14(3), С. 55 - 64

Опубликована: Фев. 22, 2025

Objective: to study pathogenetic homeostasis changes in connective tissue dysplasia substantiate its role the predisposition musculoskeletal injuries. Materials and methods: basis of this scientific-analytical review was analysis data from electronic portals PubMed-NCBI, Google Academy, Scientific e-Library.ru. Results: presented literature indicates that dysplasia, based on polygenically determined and/or environmental factors-induced defects synthesis or remodeling intercellular matrix components, manifested by formation deficient significant biomechanical properties. This is primarily affects structure functions. Depending impaired metabolism stage, a variety clinical signs indicating pathology skeletal bones their joints, failure intermuscular layers tendon-fascial complexes. Conclusions: functions are initially reduced adaptive capabilities resistance physical load, determining injury. A personalized approach choice sports activity training program will help preserve youth health, prevent re-traumatism early disability.

Язык: Английский

---

Glycosaminoglycans, Instructive Biomolecules That Regulate Cellular Activity and Synaptic Neuronal Control of Specific Tissue Functional Properties

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2554 - 2554

Опубликована: Март 12, 2025

Glycosaminoglycans (GAGs) are a diverse family of ancient biomolecules that evolved over millennia as key components in the glycocalyx surrounds all cells. GAGs have molecular recognition and cell instructive properties when attached to surface extracellular matrix (ECM) proteoglycans (PGs), which act effector molecules regulate cellular behavior. The perception mechanical cues arise from perturbations ECM microenvironment allow undertake appropriate biosynthetic responses maintain composition tissue function. PGs substituted with provide structural support weight-bearing tissues an ability withstand shear forces some contexts. This review outlines complexity functional they convey PGs. important roles cartilaginous fibrocartilages subject tension high also vascular neural tissues. Specific synaptic stabilization specificity plasticity regulation neurophysiological CNS/PNS control A better understanding GAG instructional behavior may be insightful for development GAG-based biotherapeutics designed treat dysfunction disease processes novel repair strategies following trauma. significant level sophistication many contexts, needs fully deciphered order achieve useful therapeutic product. offers exciting opportunities modern glycomics arena.

Язык: Английский

---

Adenovirus cell entry

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 45 - 73

Опубликована: Янв. 1, 2025

---