The Gut Microbiome Regulates the Psychomotor Effects and Context-Dependent Rewarding Responses to Cocaine in Germ-Free and Antibiotic-Treated Animal Models

Microorganisms, Год журнала: 2025, Номер 13(1), С. 77 - 77

Опубликована: Янв. 3, 2025

Cocaine use disorder remains a major global health concern, with growing evidence that the gut microbiome modulates drug-related behaviors. This study examines microbiome’s role in cocaine-induced psychomotor activation and context-dependent reward responses using germ-free (GF) antibiotic-treated (ABX) models. In GF mice, absence of blunted (p = 0.013), which was restored after conventionalization. mice also showed reduced cocaine-conditioned place preference (CPP) 0.002), normalized Dopaminergic function, critical for reward, microbiome-dependent, increased dopamine levels 0.009) turnover ratios ABX model, depletion both locomotion CPP ≤ 0.009), further supporting microbes modulating ABX-treated significant declines microbial diversity, shifts bacterial structure, dysregulation metabolic, immune, neurotransmitter pathways 0.0001), including alterations short-chain fatty acids gamma-aminobutyric acid metabolism. These findings highlight regulating cocaine’s rewarding effects, offering insights into potential therapeutic strategies cocaine disorder.

Язык: Английский

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Gut Microbiota‐Derived Extracellular Vesicles Influence Alcohol Intake Preferences in Rats

Journal of Extracellular Vesicles, Год журнала: 2025, Номер 14(3)

Опубликована: Март 1, 2025

ABSTRACT Growing preclinical and clinical evidence suggests a link between gut microbiota dysbiosis problematic alcohol consumption. Extracellular vesicles (EVs) are key mediators involved in bacteria‐to‐host communication. However, their potential role mediating addictive behaviour remains unexplored. This study investigates the of microbiota‐derived bacterial extracellular (bEVs) driving high bEVs were isolated from alcohol‐drinking rat strain (UChB rats), either ethanol‐naïve or following chronic consumption administered intraperitoneally orally to alcohol‐rejecting male female Wistar rats. Both types UChB‐derived increased Wistar's voluntary (three bottle choice test) up 10‐fold ( p < 0.0001), indicating that able sufficient transmit drinking across different strains. Molecular analysis revealed administration did not induce systemic brain inflammation recipient animals, suggesting intake triggered by operates through an inflammation‐independent mechanism. Furthermore, we demonstrate vagus nerve mediates bEV‐induced increase consumption, as bilateral vagotomy completely abolished induced both injected bEVs. Thus, this identifies novel mechanism underlying microbiota‐induced nerve‐dependent manner.

Язык: Английский

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Gut microbiome depletion modulates cocaine-induced behavioral and transcriptional responses in female mice

Journal of Neuroimmunology, Год журнала: 2025, Номер unknown, С. 578609 - 578609

Опубликована: Апрель 1, 2025

Язык: Английский

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Clinical and Preclinical Evidence for Gut Microbiome Mechanisms in Substance Use Disorders

Biological Psychiatry, Год журнала: 2023, Номер 95(4), С. 329 - 338

Опубликована: Авг. 10, 2023

Язык: Английский

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The microbial community dynamics of cocaine sensitization in two behaviorally divergent strains of collaborative cross mice

Genes Brain & Behavior, Год журнала: 2023, Номер 22(3)

Опубликована: Апрель 27, 2023

Abstract The gut‐brain axis is increasingly recognized as an important pathway involved in cocaine use disorder. Microbial products of the murine gut have been shown to affect striatal gene expression, and depletion microbiome by antibiotic treatment alters cocaine‐induced behavioral sensitization C57BL/6J male mice. Some reports suggest that correlated with drug self‐administration behavior Here, we profile composition naïve its response two collaborative cross (CC) strains. These strains display extremely divergent responses sensitization. A high‐responding strain, CC004/TauUncJ (CC04), has a contains greater amount Lactobacillus than cocaine‐nonresponsive strain CC041/TauUncJ (CC41). CC41 characterized abundance Eisenbergella , Robinsonella Ruminococcus. In cocaine, CC04 increased Barnsiella population, while displays no significant changes. PICRUSt functional analysis potential shows number modules altered after exposure specifically those encoding for tryptophan synthesis, glutamine metabolism, menaquinone synthesis (vitamin K2). Depletion revealed cocaine‐sensitization following antibiotics female Depleting males infusions during intravenous dose–response curve. Together these data genetic differences cocaine‐related behaviors may involve microbiome.

Язык: Английский

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Dynamic Human Gut Microbiome and Immune Shifts During an Immersive Psychosocial Therapeutic Program

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 27, 2024

Depression is a leading cause of disability worldwide yet its underlying factors, particularly microbial associations, are poorly understood.

Язык: Английский

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Dynamic human gut microbiome and immune shifts during an immersive psychosocial intervention program

Brain Behavior and Immunity, Год журнала: 2024, Номер 125, С. 428 - 443

Опубликована: Дек. 17, 2024

Язык: Английский

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