Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(11)
Опубликована: Март 8, 2023
The
ocean
is
a
net
source
of
the
greenhouse
gas
and
ozone-depleting
substance,
nitrous
oxide
(N2O),
to
atmosphere.
Most
that
N2O
produced
as
trace
side
product
during
ammonia
oxidation,
primarily
by
ammonia-oxidizing
archaea
(AOA),
which
numerically
dominate
community
in
most
marine
environments.
pathways
production
their
kinetics,
however,
are
not
completely
understood.
Here,
we
use
15N
18O
isotopes
determine
kinetics
nitrogen
(N)
oxygen
(O)
atoms
model
AOA
species,
Nitrosopumilus
maritimus.
We
find
apparent
half
saturation
constants
nitrite
comparable,
suggesting
both
processes
enzymatically
controlled
tightly
coupled
at
low
concentrations.
constituent
derived
from
ammonia,
nitrite,
O2,
H2O
via
multiple
pathways.
Ammonia
primary
N
N2O,
but
its
contribution
varies
with
ratio.
ratio
45N2O
46N2O
(i.e.,
single
or
double
labeled
N)
substrate
ratio,
leading
widely
varying
isotopic
signatures
pool.
O2
for
O
atoms.
In
addition
previously
demonstrated
hybrid
formation
pathway,
found
substantial
hydroxylamine
while
reduction
an
insignificant
N2O.
Our
study
highlights
power
dual
15N-18O
isotope
labeling
disentangle
microbes,
implications
interpretation
regulation
sources.
Critical Reviews in Environmental Science and Technology,
Год журнала:
2022,
Номер
53(2), С. 161 - 176
Опубликована: Март 20, 2022
The
discovery
of
complete
ammonia
oxidizers,
comammox
Nitrospira,
represents
a
breakthrough
in
the
history
nitrification
research.
Nitrospira
inopinata,
which
was
obtained
from
an
aquatic
ecosystem,
is
only
pure
bacterial
isolate
reported
so
far.
Ammonia
oxidation
kinetics
N.
inopinata
indicated
that
they
prefer
oligotrophic
lifestyle
and
may
directly
compete
with
ammonia-oxidizing
archaea
(AOA),
but
representatives
terrestrial
ecosystems
are
lacking.
Current
studies
demonstrate
functionally
dominant
separated
most
sequences
engineering
systems.
Clades
A
B
seemingly
have
different
ecological
preferences
soils,
possibly
due
to
their
ammonium
uptake
Analyses
evolutionary
indicate
some
genes
involved
could
be
laterally
transferred
β-ammonia-oxidizing
bacteria
(AOB).
Some
species
similar
AOB
active
niches
where
previously
considered
dominated
by
AOB.
Taken
together,
this
review
summarizes
recent
findings
biogeographical
distribution
preference
large-scale
soil
surveys,
responses
nitrogen
application
soil,
putative
mechanisms
underpinning
niche
Nitrospira.
These
suggest
not
strictly
both
copiotrophic
broader
breadth.
Comammox
abundant
environments.
profoundly
expanded
our
knowledge
environmental
relative
contribution
soils.
Soil Biology and Biochemistry,
Год журнала:
2022,
Номер
166, С. 108563 - 108563
Опубликована: Янв. 17, 2022
Nitrous
oxide
(N2O)
production
in
tropical
soils
cultivated
with
sugarcane
is
associated
ammonia-oxidizing
bacteria
(AOB)
and
fungal
denitrifiers.
However,
the
taxonomic
identities
community
diversities,
compositions,
structures
of
AOB
denitrifiers
these
are
not
known.
Here,
we
examined
effects
applying
different
concentrations
an
organic
recycled
residue
(vinasse:
regular
non-concentrated
or
5.8-fold
concentrated)
on
dynamics
denitrifier
diversity
composition
greenhouse
gas
emissions
during
cycle
two
seasons,
rainy
dry.
DNA
was
extracted
from
soil
samples
collected
at
six
timepoints
to
determine
amoA-AOB
nirK-fungal
by
amplicon
sequencing
gene-specific
primers.
Bacterial
archaeal
amoA,
bacterial
nirK,
nirS
nosZ,
total
(16S
rRNA)
fungi
(18S
were
quantified
real-time
PCR,
N2O
CO2
measured.
The
genes
nirK
clade
II
correlated
emissions,
followed
nirK.
application
inorganic
nitrogen
fertilizer
combined
residue,
regardless
concentration,
did
affect
structure
communities
but
increased
their
abundances
emissions.
Nitrosospira
sp.
dominant
AOB,
while
unclassified
Furthermore,
affected
season,
dominance
uncultured
season
genera
Chaetomium
dry
season.
Nitrosospira,
Chaetomium,
Talaromyces
purpureogenus,
Fusarium
seemed
be
main
governing
studied
soils.
These
results
highlight
importance
deciphering
players
demonstrate
impact
fertilization
microbial
N
functions.
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(11)
Опубликована: Март 8, 2023
The
ocean
is
a
net
source
of
the
greenhouse
gas
and
ozone-depleting
substance,
nitrous
oxide
(N2O),
to
atmosphere.
Most
that
N2O
produced
as
trace
side
product
during
ammonia
oxidation,
primarily
by
ammonia-oxidizing
archaea
(AOA),
which
numerically
dominate
community
in
most
marine
environments.
pathways
production
their
kinetics,
however,
are
not
completely
understood.
Here,
we
use
15N
18O
isotopes
determine
kinetics
nitrogen
(N)
oxygen
(O)
atoms
model
AOA
species,
Nitrosopumilus
maritimus.
We
find
apparent
half
saturation
constants
nitrite
comparable,
suggesting
both
processes
enzymatically
controlled
tightly
coupled
at
low
concentrations.
constituent
derived
from
ammonia,
nitrite,
O2,
H2O
via
multiple
pathways.
Ammonia
primary
N
N2O,
but
its
contribution
varies
with
ratio.
ratio
45N2O
46N2O
(i.e.,
single
or
double
labeled
N)
substrate
ratio,
leading
widely
varying
isotopic
signatures
pool.
O2
for
O
atoms.
In
addition
previously
demonstrated
hybrid
formation
pathway,
found
substantial
hydroxylamine
while
reduction
an
insignificant
N2O.
Our
study
highlights
power
dual
15N-18O
isotope
labeling
disentangle
microbes,
implications
interpretation
regulation
sources.
