Identification and Characterization of Troponin T Associated with Development, Metabolism and Reproduction in Tribolium castaneum
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(6), С. 2786 - 2786
Опубликована: Март 19, 2025
As
a
tropomyosin-binding
component,
troponin
T
(TnT)
is
essential
for
the
Ca2+
regulation
of
striated
muscles’
contraction
and
locomotion
activity,
but
its
impacts
on
growth
development
insects
have
rarely
been
reported.
In
this
study,
TnT
was
identified
functionally
characterized
in
Tribolium
castaneum
by
RNA
interference
(RNAi)
transcriptome
analysis.
The
T.
contained
1152
bp
open
reading
frame
encoding
383
amino
acids.
It
displayed
highest
expression
late
pupae
highly
expressed
integument
CNS.
Both
larval
early
pupal
injection
dsTnT
led
to
100%
cumulative
mortality
before
pupal–adult
transition.
Late
RNAi
caused
26.01
±
4.29%
mortality;
survivors
successfully
became
adults,
49.71
6.51%
died
10
days
with
dried
shriveled
abdomen,
poorly
developed
reproductive
system
no
offspring.
Additionally,
sequencing
results
indicated
that
key
ecdysteroid
juvenile
hormone
biosynthesis
genes
(CYP314A1,
aldehyde
dehydrogenase
family
3
member
B1
farnesol
dehydrogenase)
were
affected,
as
well
several
cuticle
protein,
nutrition
metabolism
immune-related
genes,
suggesting
may
play
prominent
roles
development,
reproduction
affecting
these
pathways.
This
study
could
provide
brand-new
target
gene
strategy
pest
control.
Язык: Английский
Cystatin antibodies interfere with ovary development in Haemaphysalis doenitzi (Acari: Ixodidae)
PLoS neglected tropical diseases,
Год журнала:
2025,
Номер
19(5), С. e0013064 - e0013064
Опубликована: Май 7, 2025
Anti-tick
vaccines
are
gaining
attention
as
a
strategy
to
prevent
tick
infestations
by
activating
the
immune
response
of
host.
Antibodies
produced
host
inhibit
growth
and
reproduction,
but
molecular
mechanism
remains
be
clarified.
In
this
study,
we
investigated
effects
cystatin
antibodies
on
ovarian
function
Haemaphysalis
doenitzi
.
Histological
analysis
revealed
that
exposure
resulted
in
significant
reduction
number
eggs
caused
severe
damage
tissue
structure.
Immunofluorescence
experiments
confirmed
expression
within
ovary.
Proteomics
phosphoproteomics
identified
31
10
differentially
expressed
proteins
relevant
pathways,
respectively.
These
changes
protein
levels
were
found
regulated
various
mechanisms,
including
ribosomes
activity,
regulation
actin
cytoskeleton,
RNA
transport,
TCA
cycle,
drug
metabolism,
mTOR
signaling
pathways.
Notably,
there
was
high
tropomyosin
low
glutathione
S-transferase
(GST)
during
detoxification.
Enzyme
activity
assays
indicated
down-regulation
GST
enzyme
immunized
group,
suggesting
impaired
detoxification
capacity
ticks.
Both
successfully
cloned
designated
HD-TPMa
HD-GSTa,
interference
(RNAi)
knocked
down
target
gene.
Ticks
subjected
immersion
exhibited
significantly
increased
mortality
rate
after
72
hours.
This
study
elucidated
which
development
ovaries,
providing
an
important
scientific
basis
for
effective
ovary
control
strategies.
Язык: Английский
RNAi‐mediated pest control targeting the Troponin I (wupA) gene in sweet potato weevil, Cylas formicarius
Insect Science,
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 11, 2024
Abstract
The
sweet
potato
weevil
(
Cylas
formicarius
)
is
a
critical
pest
producing
enormous
global
losses
in
crops.
Traditional
management
approaches
for
weevil,
primarily
using
chemical
pesticides,
causes
pollution,
food
safety
issues,
and
harming
natural
enemies.
While
RNA
interference
(RNAi)
promising
environmentally
friendly
approach
to
control,
its
efficacy
controlling
the
has
not
been
extensively
studied.
In
this
study,
we
selected
potential
target
C.
,
Troponin
I
gene
wupA
),
which
essential
musculature
composition
crucial
fundamental
life
activities.
We
determined
that
abundantly
expressed
throughout
all
developmental
stages
of
weevil.
evaluated
efficiency
double‐stranded
RNAs
silencing
via
microinjection
oral
feeding
larvae
at
different
ages.
Our
findings
demonstrate
both
significantly
reduced
expression
produced
high
mortality.
Moreover,
1st
instar
administered
ds
exhibited
significant
growth
inhibition.
assessed
toxicity
on
no‐target
insect
silkworm
safety.
study
indicates
knockdown
can
inhibit
development
offer
control.
Язык: Английский
Hexamerin and allergen are required for female reproduction in the American cockroach, Periplaneta americana
Insect Science,
Год журнала:
2023,
Номер
31(1), С. 186 - 200
Опубликована: Июнь 16, 2023
Abstract
Reproduction
is
of
great
importance
for
the
continuation
species.
In
insects,
fat
body
major
tissue
nutrient
storage
and
involved
in
vitellogenesis,
which
essential
female
reproduction.
Here,
2
proteins,
hexamerin
allergen,
were
separated
from
bodies
adult
American
cockroaches
(
Periplaneta
americana
)
identified
as
encoding
733
amino
acids
with
molecular
weight
87.88
kDa
686
82.18
kDa,
respectively.
The
genes
these
proteins
are
mainly
expressed
body.
RNA
interference‐mediated
knockdown
Hexamerin
Allergen
early
stage
first
reproductive
cycle
females
suppressed
vitellogenesis
ovarian
maturation,
indicating
that
controlling
Importantly,
expression
was
repressed
by
juvenile
hormone
(JH)
receptor
gene
Met
primary
response
Kr‐h1
,
induced
methoprene,
a
JH
analog,
both
vivo
vitro
experiments.
Altogether,
we
have
determined
allergen
play
an
important
role
promoting
reproduction
cockroach.
their
signaling.
Our
data
reveal
novel
mechanism
necessary
JH‐stimulated
Язык: Английский