Microglial C/EBPβ-Fcgr1 regulatory axis blocking inhibits microglial pyroptosis and improves neurological recovery
Journal of Neuroinflammation,
Год журнала:
2025,
Номер
22(1)
Опубликована: Янв. 31, 2025
CAAT/Enhancer
Binding
Protein
β
(C/EBPβ)
is
associated
with
inflammatory
responses
in
neurodegenerative
pathologies,
particularly
the
brain.
However,
regulatory
role
of
C/EBPβ
spinal
cord
injury
and
its
impact
on
neurological
recovery
remain
unknown.
In
this
study,
we
observed
significant
upregulation
microglia
after
mice
was
neuroinflammation.
Knocking
down
attenuated
pyroptosis,
reduced
production
proinflammatory
cytokines,
inhibited
neuronal
apoptosis.
Mechanistically,
promoted
transcription
Fcgr1,
which
involved
activating
pyroptosis.
both
in-vivo
in-vitro
experiments,
knocking
Cebpb
or
pyroptosis
inhibitor
VX765
apoptosis
improved
mice.
These
findings
indicate
that
functions
as
a
key
regulator
participates
pyroptosis-mediated
neuroinflammation
by
Fcgr1
transcription.
Язык: Английский
RNA production of IgG receptors is present in podocytes and varies depending on glycemia – preliminary results on Fc gamma receptor presence in kidney cells
Gene,
Год журнала:
2025,
Номер
unknown, С. 149501 - 149501
Опубликована: Апрель 1, 2025
Язык: Английский
ADCC: the rock band led by therapeutic antibodies, tumor and immune cells
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 16, 2025
Antibody-dependent
cellular
cytotoxicity
(ADCC)
is
a
critical
mechanism
by
which
therapeutic
antibodies
leverage
the
immune
system
to
target
and
eliminate
cancer
cells.
The
key
agents
of
ADCC
are
natural
killer
(NK)
cells,
specifically
targeting
antibody-covered
cells
through
CD16
receptor.
While
other
Fc
receptors
can
contribute
enhance
ADCC,
NK
receptor
crucial
for
efficacy
therapies
such
as
trastuzumab,
cetuximab
rituximab.
Co-culture
assays
essential
understanding
mechanisms
these
therapies,
overcoming
resistance
optimizing
novel
antibodies.
This
review
highlights
importance
measuring
assess
Here
we
also
present
various
in
vitro
models
assay
methodologies
available
studying
comparing
strengths
limitations
approaches
like
using
PBMCs
better
reflect
real-life
conditions
or
cell
lines
standardization.
It
covers
different
readouts
either
focusing
on
effector
activation,
including
reporter
degranulation
killing,
molecule
release
assays,
flow
cytometry
immunofluorescence
techniques.
Selecting
best
model
translational
significance
antibody
research.
Язык: Английский