EPAS1, a hypoxia‐ and ferroptosis‐related gene, promotes malignant behaviour of cervical cancer by ceRNA and super‐enhancer
Journal of Cellular and Molecular Medicine,
Год журнала:
2024,
Номер
28(9)
Опубликована: Май 1, 2024
Abstract
Hypoxia
and
Ferroptosis
are
associated
with
the
malignant
behaviour
of
cervical
cancer.
Endothelial
PAS
domain‐containing
protein
1
(EPAS1)
contributes
to
progression
EPAS1
plays
important
roles
in
hypoxia
ferroptosis.
Using
GEO
dataset,
machine‐learning
algorithms
were
used
screen
for
hypoxia‐
ferroptosis‐related
genes
(HFRGs)
was
identified
as
hub
gene.
qPCR
WB
investigate
expression
normal
cancer
tissues.
The
proliferation,
invasion
migration
cells
HeLa
SiHa
cell
lines
detected
using
CCK8,
transwell
wound
healing
assays,
respectively.
Apoptosis
by
flow
cytometry.
A
dual‐luciferase
assay
analyse
MALAT1‐miR‐182‐5P‐EPAS1
mRNA
axis
core
promoter
elements
super‐enhancer.
significantly
overexpressed
could
increase
invasion,
reduce
apoptosis
cell.
According
double‐luciferase
assay,
regulated
MALAT1‐Mir‐182‐5p‐EPAS1
axis.
is
super‐enhancers.
Double‐luciferase
showed
that
super‐enhancer
E1
E3.
EPAS1,
an
HFRG,
promotes
cells.
super‐enhancers
MALAT1‐miR‐182‐5P‐
may
be
a
target
diagnosis
treatment
Язык: Английский
The roles of enhancer, especially super-enhancer-driven genes in tumor metabolism and immunity
International Journal of Biological Macromolecules,
Год журнала:
2025,
Номер
unknown, С. 142414 - 142414
Опубликована: Март 1, 2025
Язык: Английский
Harnessing single-cell and multi-omics insights: STING pathway-based predictive signature for immunotherapy response in lung adenocarcinoma
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 16, 2025
Background
Lung
adenocarcinoma
is
the
most
prevalent
type
of
small-cell
carcinoma,
with
a
poor
prognosis.
For
advanced-stage
patients,
efficacy
immunotherapy
suboptimal.
The
STING
signaling
pathway
plays
pivotal
role
in
lung
adenocarcinoma;
therefore,
further
investigation
into
relationship
between
and
warranted.
Methods
We
conducted
comprehensive
analysis
integrating
single-cell
RNA
sequencing
(scRNA-seq)
data
bulk
transcriptomic
profiles
from
public
databases
(GEO,
TCGA).
pathway-related
genes
were
identified
through
Genecard
database.
Advanced
bioinformatics
analyses
using
R
packages
(Seurat,
CellChat)
revealed
heterogeneity,
intercellular
communication
networks,
immune
landscape
characteristics.
developed
signature
(STINGsig)
101
machine
learning
frameworks.
functional
significance
ERRFI1,
key
component
STINGsig,
was
validated
mouse
models
multicolor
flow
cytometry,
particularly
examining
its
enhancing
antitumor
immunity
potential
synergy
α-PD1
therapy.
Results
Our
characterized
15
distinct
cell
populations,
including
epithelial
cells,
macrophages,
fibroblasts,
T
B
endothelial
each
unique
marker
gene
profiles.
activity
scoring
elevated
activation
neutrophils,
contrasting
lower
inflammatory
macrophages.
Cell-cell
demonstrated
enhanced
interaction
networks
high-STING-score
evident
fibroblasts
cells.
STINGsig
showed
robust
prognostic
value
microenvironment
characteristics
risk
groups.
Notably,
ERRFI1
knockdown
experiments
confirmed
significant
modulating
therapy
response.
Conclusion
STING-related
exhibited
expression
levels
across
high-score
cells
showing
tumor-promoting
pathways,
active
interactions,
enrichment
IFI27+
In
contrast,
low-score
associated
phenotypes
reduced
activity.
(STINGsig),
which
linked
to
microenvironment.
Through
vivo
experiments,
we
that
critical
within
significantly
enhances
synergizes
cancer
model,
underscoring
therapeutic
responses.
Язык: Английский
Emerging role of tumor microenvironmental nutrients and metabolic molecules in ferroptosis: Mechanisms and clinical implications
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
179, С. 117406 - 117406
Опубликована: Сен. 9, 2024
Язык: Английский
Ferroptosis-related gene transferrin receptor protein 1 expression correlates with the prognosis and tumor immune microenvironment in cervical cancer
PeerJ,
Год журнала:
2024,
Номер
12, С. e17842 - e17842
Опубликована: Авг. 6, 2024
Ferroptosis
is
a
non-apoptotic
iron-dependent
form
of
cell
death
implicated
in
various
cancer
pathologies.
However,
its
precise
role
tumor
growth
and
progression
cervical
(CC)
remains
unclear.
Transferrin
receptor
protein
1
(TFRC),
key
molecule
associated
with
ferroptosis,
has
been
identified
as
influencing
broad
range
pathological
processes
different
cancers.
the
prognostic
significance
TFRC
CC
The
present
study
utilized
bioinformatics
to
explore
ferroptosis-related
gene
prognosis
CC.
Язык: Английский
Unveiling epigenetic regulatory elements associated with breast cancer development
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 15, 2024
Breast
cancer
is
the
most
common
in
women
and
2nd
worldwide,
yearly
impacting
over
2
million
females
causing
650
thousand
deaths.
It
has
been
widely
studied,
but
its
epigenetic
variation
not
entirely
unveiled.
We
aimed
to
identify
mechanisms
expression
of
breast
related
genes
detect
new
potential
biomarkers
therapeutic
targets.
considered
The
Cancer
Genome
Atlas
database
with
800
samples
several
omics
datasets
such
as
mRNA,
miRNA,
DNA
methylation,
which
we
used
select
2701
features
that
were
statistically
significant
differ
between
control
using
Monte
Carlo
Feature
Selection
Interdependency
Discovery
algorithm,
from
an
initial
total
417,486.
Their
biological
impact
on
cancerogenesis
was
confirmed
using:
statistical
analysis,
natural
language
processing,
linear
machine
learning
models
well
as:
transcription
factors
identification,
drugs
3D
chromatin
structure
analyses.
Classification
vs
selected
returned
high
classification
weighted
Accuracy
0.91
0.98
depending
feature-type:
algorithm.
In
general,
showed
lower
differentially
expressed
increased
Язык: Английский