ClinicoEconomics and Outcomes Research, Год журнала: 2024, Номер Volume 16, С. 847 - 868
Опубликована: Дек. 1, 2024
To evaluate chronic conditions as leading predictors of economic burden over time among older adults with incident primary Merkel Cell Carcinoma (MCC) using machine learning methods.
Язык: Английский
Springer eBooks, Год журнала: 2025, Номер unknown, С. 1 - 75
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Biology Direct, Год журнала: 2024, Номер 19(1)
Опубликована: Июнь 7, 2024
Abstract Background The molecular mechanisms of osteosarcoma (OS) are complex. In this study, we focused on the functions melanoma cell adhesion molecule (MCAM), methyltransferase 3 (METTL3) and insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1) in OS development. Methods qRT-PCR assay western blot were performed to determine expression MCAM, METTL3, IGF2BP1 YY1. MTT colony formation conducted assess proliferation. Cell apoptosis, invasion migration evaluated by flow cytometry analysis, transwell wound-healing assay, respectively. Methylated RNA Immunoprecipitation (MeRIP), dual-luciferase reporter, Co-IP, RIP ChIP assays analyze relationships METTL3 MCAM tumor explored through vivo experiments. Results was upregulated OS, overexpression promoted growth, inhibited apoptosis. demonstrated mediate m6A methylation MCAM. Functionally, or silencing progression, while ameliorated effects. Transcription YY1 transcription level regulated cells. Additionally, deficiency suppressed vivo, abated effect. Conclusion YY1/METTL3/IGF2BP1/MCAM axis aggravated development, which might provide novel therapy targets for OS.
Язык: Английский
Процитировано
3
Molecular Medicine, Год журнала: 2024, Номер 30(1)
Опубликована: Окт. 22, 2024
Abstract Background Overconsumption of retinoic acid (RA) or its analogues/derivatives has been linked to severe craniomaxillofacial malformations, such as cleft palate and midface hypoplasia. It noted that RA disturbed the proliferation migration embryonic palatal mesenchymal (EPM) cells in these yet exact mechanisms underlying disruptions remained unclear. Methods A model (RA)-induced fetal mice was successfully established. Histological alterations were evaluated using Hematoxylin Eosin (H&E) staining RNA situ hybridization (RNAscope). Cellular levels quantified via Cell Counting Kit-8 (CCK-8) assay EdU incorporation assay, while cell capabilities investigated wound healing Transwell assays. Mitochondrial functions assessed through Mito-Tracker fluorescence, mitochondrial reactive oxygen species (ROS) measurement, ATP level quantification, DNA (mtDNA) copy number analysis. Differential gene expression associated signaling pathways identified bioinformatics Alterations transcriptional translational Lhx6 genes with mitophagy quantitative PCR (qPCR) Western blot analysis, respectively. morphology autophagosomes within examined transmission electron microscopy (TEM). Results Abnormal development mice, along impaired human (HEPM) cells, affecting function concomitant downregulation Lhx6. Knockdown HEPM resulted altered proliferation, migration, function. Conversely, aberrant function, observed RA-induced ameliorated by overexpression Subsequent research demonstrated dysfunction modulating PINK1/Parkin-mediated mitophagy, thereby activating MAPK pathways. Conclusion is essential for homeostasis tuning Downregulation transcriptionally disturbs homeostasis, which turn leads defect ultimately causing palate. Graphical abstract
Язык: Английский
Процитировано
3
Basic and Clinical Andrology, Год журнала: 2024, Номер 34(1)
Опубликована: Сен. 17, 2024
Cryopreservation of human spermatozoa is a widely used technique in the assisted reproduction technology laboratory for storage gametes later use, fertility preservation and sperm donation programs. can cause damage to membrane, cytoskeletal, acrosome increased oxidative stress, DNA transcriptome changes. To assess impact time on frozen spermatozoa, semen samples were collected from 24 normospermic donors whom 13 had cryostored short-time (1 week) 11 long-time (median 9 years).
Язык: Английский
Процитировано
2
Microbial Biotechnology, Год журнала: 2024, Номер 17(10)
Опубликована: Окт. 1, 2024
Merkel cell polyomavirus (MCPyV) is the foremost causative factor of carcinoma (MCC), a rare yet highly aggressive skin cancer. Although evaluation circulating IgG antibodies against LT/sT oncoproteins clinically useful for MCC diagnosis/prognosis, limited number assays identifying such have been developed. Herein, novel indirect immunoassay with synthetic epitopes/mimotopes MCPyV was computationally designed and experimentally validated on control sera from healthy individuals patients. Upon computational design five peptides, performance in detecting anti-oncoprotein IgGs MCPyV-positive -negative evaluated. The afterwards extended individuals, and, longitudinal analysis, Performance properties as sensitivity specificity positive/negative predictive values were adequate. Receiver-operating characteristic (ROC) curves indicated that areas under (AUCs) within low/moderately accurate ranges. Immunoassay repeatable, reproducible accurate. As expected, serum prevalence low (2%-5%). Anti-oncoprotein slightly increased when patients experienced partial tumour remission and/or stable disease, compared to baseline. Our data indicate newly developed reliable both
Язык: Английский
Процитировано
1
ClinicoEconomics and Outcomes Research, Год журнала: 2024, Номер Volume 16, С. 847 - 868
Опубликована: Дек. 1, 2024
To evaluate chronic conditions as leading predictors of economic burden over time among older adults with incident primary Merkel Cell Carcinoma (MCC) using machine learning methods.
Язык: Английский
Процитировано
0