Genome-wide association study reveals genetic loci for seed density per silique in rapeseed (Brassica napus L.)

Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)

Опубликована: Март 20, 2025

Язык: Английский

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Phospholipase-mediated phosphate recycling during plant leaf senescence

Genome biology, Год журнала: 2024, Номер 25(1)

Опубликована: Июль 29, 2024

Phosphorus is a macronutrient necessary for plant growth and development its availability efficient use affect crop yields. Leaves are the largest tissue that uses phosphorus in plants, membrane phospholipids main source of cellular usage. Here we identify key process recycling mediated by phospholipid hydrolysis during leaf senescence. Our results indicate over 90% lipid phosphorus, accounting more than one-third total recycled from senescent leaves before falling off plants. Nonspecific phospholipase C4 (NPC4) Dζ2 (PLDζ2) highly induced senescence, knockouts PLDζ2 NPC4 decrease loss delay Conversely, overexpression accelerates promoting remobilization to young tissues growth. We also show this conserved These PLDζ2- NPC4-mediated promotes growing hydrolysis-mediated improves efficiency

Язык: Английский

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Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus

Cell Reports, Год журнала: 2025, Номер 44(2), С. 115280 - 115280

Опубликована: Фев. 1, 2025

Highlights•A pleiotropic QTL gene is identified by GWAS for seed coat content and oil content•The expression of BnaA09.MYB52 predominant in the during development•BnaA09.MYB52 directly targets PMEI14 BAN to affect SOC SCC•BnaMYB52 mutation results increased SOC, decreased SCC, reduced lignin contentSummarySeed synthesized embryo, which surrounded coat. However, genetic molecular mechanisms how development affects (SOC) remains less studied. Through genome-wide association studies Brassica napus accessions, we identify transcription factor as a candidate underlying ZS11_qSCC.A09. Knocking out BnaMYB52 7.7%–8.2% reduction (SCC) but 12.3%–13.4% increase whereas overexpression leads opposite effects. Intriguingly, has development. activates BAN. Mutant analyses indicate that 4.3%–7.7%, while thickness shows 12.9%–22.8% ban pmei14 mutants. Thus, our findings mechanism MYB52-BAN/PMEI14 modules regulating SCC provide potential target improvement B. napus.Graphical abstract

Язык: Английский

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A chromosome-level genome assembly of Mylabris sibirica Fischer von Waldheim, 1823 (Coleoptera, Meloidae)

Scientific Data, Год журнала: 2025, Номер 12(1)

Опубликована: Фев. 14, 2025

Язык: Английский

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Integration of GWAS and transcriptome analysis to identify temperature-dependent genes involved in germination of rapeseed (Brassica napus L.)

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Март 3, 2025

Low temperature germination (LTG) is one of crucial agronomic traits for field-grown rapeseed in the Yangtze River Basin, where delayed sowing frequently exposes to cold stress. Because its importance, genetic basis underlying under different temperatures has been continuously focused. By long-term field observation, we screened out two cultivars with significantly LTG performance (JY1621 and JY1605) lab conditions, which therefore were further used transcriptome sequencings at three key timepoints normal low temperatures. Comparative analysis among multiple groups differentially expressed genes (DEGs) revealed a set either e arly or l ate t emperature r esponse g ermination (ETRG LTRG) genes, as well c ol d - olerant (CDT) em p erature- i nsensitive (TPI) candidate regulators stages. Furthermore, performed genome-wide association study (GWAS) using index 273 accessions identified 24 significant loci associated potential Through integrated sequencing GWAS, series involved temperature-dependent germination. Based on comprehensive analysis, hypothesized that BnaA3.CYP77A4 BnaA3.NAC078 could be important due their expression patterns haplotype distributions. This multi-omics provided required robust germination, considered low-temperature breeding rapeseed.

Язык: Английский

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Improved Salt Tolerance in Brassica napus L. Overexpressing a Synthetic Deinocuccus Stress-Resistant Module DICW

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(6), С. 2500 - 2500

Опубликована: Март 11, 2025

Salt stress adversely impacts plant physiology by causing ionic, osmotic, and oxidative stress, ultimately hindering growth yield. The genus Deinococcus contains unique resistance genes, previous studies have shown that proteins such as IrrE, Csp, WHy enhance tolerance in plants microbial cells. However, their role Brassica napus L. (oilseed rape) remains unexamined. In this study, a synthetic stress-resistance module, DICW, was constructed using the Deinococcus-derived genes heterologously overexpressed B. to assess its impact on salt tolerance. results demonstrated DICW module significantly improved seed germination seedling under stress. Transgenic exhibited reduced membrane damage, higher leaf relative water content, enhanced accumulation of osmoregulatory substances, elevated antioxidant enzyme activity compared wild-type plants. Additionally, qRT-PCR analysis revealed upregulation stress-related (BnRD29A, BnP5CS, BnKIN1, BnLEA1, BnNHX1, BnSOS1) enzyme-related (BnSOD, BnPOD, BnCAT) transgenic lines. conclusion, plays crucial enhancing regulating responses mechanisms. This study provides valuable molecular insights into improving survival saline environments.

Язык: Английский

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Applications of Genomics and Genome-Editing Technologies in Oil Crops

Опубликована: Янв. 1, 2025

Язык: Английский

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Fine mapping and candidate gene analysis of the major QTL qSW-A03 for seed weight in Brassica napus

Theoretical and Applied Genetics, Год журнала: 2025, Номер 138(4)

Опубликована: Март 17, 2025

Язык: Английский

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Genetic and transcriptome analyses of the effect of genotype-by-environment interactions on Brassica napus seed oil content

The Plant Cell, Год журнала: 2025, Номер 37(4)

Опубликована: Март 26, 2025

Abstract The molecular basis underlying crop traits is complex, with gene-by-environment interactions (GEIs) affecting phenotypes. However, quantitative trait nucleotide (QTN)-by-environment (QEIs) and GEIs for seed oil content (SOC) in crops are rare. Here, we detected 11 environmentally specific 10 stable additive QTNs QEIs SOC rapeseed (Brassica napus) using genome-wide association studies. Weighted gene co-expression network analysis identified 8 Environmental-Developmental Gene Modules which the eigengenes correlated environment explained a large proportion of variance expression. By incorporating information from multi-omics dataset, 17 candidate genes were predicted. We mined 1 GEI candidate, LIGHT-DEPENDENT SHORT HYPOCOTYLS5 (LSH5), around QTN qspOC.A02.1 QEI qeOC.A02.1 by climatic indices as covariates. BnaA02.LSH5 was highly expressed early development, its expression varied significantly across planting sites, trend opposite to light-related indices. BnaA02.lsh5 BnaC02.lsh5 double mutants had lower SOC, hypocotyl length, photosynthesis, carbon- energy-related metabolites compared wild type. Moreover, transcriptionally directly repressed BnaA02.pMDH2 fatty acid β-oxidation photosynthetic electron transport. propose that BnaLSH5 affects accumulation response light intensity. This study provides creating high-oil germplasm adapted environments.

Язык: Английский

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Transcriptional regulation of transcription factor genes WRI1 and LAFL during Brassica napus seed development

PLANT PHYSIOLOGY, Год журнала: 2024, Номер unknown

Опубликована: Июль 23, 2024

Abstract The WRINKLED1 (WRI1) and LAFL (LEAFY COTYLEDON1 [LEC1], ABSCISIC ACID INSENSITIVE3 [ABI3], FUSCA3 [FUS3], LEC2) transcription factors play essential roles in governing seed development oil biosynthesis. To gain a comprehensive understanding of the transcriptional regulation WRI1 LAFL, we conducted genome-wide association studies for expression profiles developing seeds at 20 40 days after flowering (DAF) using 302 rapeseed (Brassica napus) accessions. We identified total 237 quantitative trait nucleotides (eQTNs) 51 QTN-by-environment interactions (eQEIs) associated with LAFL. Around these eQTNs eQEIs, pinpointed 41 8 candidate genes known regulations or protein their traits, respectively. Based on RNA-sequencing assay transposase-accessible chromatin high-throughput sequencing data, employed Extreme Gradient Boosting Basenji models which predicted 15 potentially regulating further validated predictions via tissue profile, haplotype analysis, correlation analysis verified activation activity BnaC03.MYB56 (R2R3-MYB factor 56) BnaA09.LEC1 by dual-luciferase reporter yeast 1-hybrid assays. BnaA10.AGL15 (AGAMOUS-LIKE 15), BnaC04.VAL1 (VIVIPAROUS1/ABSCISIC INSENSITIVE3-LIKE 1), BnaC03.MYB56, BnaA10.MYB56 were coexpressed DAF M35, key module positive MYB56 accumulation Arabidopsis (Arabidopsis thaliana) mutants. This study not only delivers framework future eQEI identification but also offers insights into developmental accumulation.

Язык: Английский

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