An optimized protocol for in vitro regeneration and genetic transformation of broomcorn millet DOI Creative Commons

Zhaolan Cui,

Wenmin Wei,

Xinqi Han

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 2, 2024

Abstract Background Broomcorn millet has many advantages, such as abiotic stress resistance, a short growth cycle and high nutritional value. However, due to the lack of efficient genetic transformation methods for broomcorn millet, characterization genes related important traits lags behind that other crop species. Therefore, establishing in vitro regeneration is essential. Results In this study, we used mature seeds from sequenced cultivar 'Longmi 4' explants optimized their methods. The optimal hormone concentrations embryogenic callus induction medium were 2.5 mg/L 2,4-D 0.5 BAP. shoot media 2 kinetin a-naphthaleneacetic acid. Additionally, cocultivation time was 3 days, hygromcin concentration putative transgenic selection 45 mg/L. efficiency 21.25% after our modification approach. Conclusions Here, present simple highly Agrobacterium-mediated protocol millet. Our work provides tool traits, well new strategy breeding.

Язык: Английский

The role of pangenomics in orphan crop improvement DOI Creative Commons
Haifei Hu, Junliang Zhao, William J. W. Thomas

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

Global food security depends heavily on a few staple crops, while orphan despite being less studied, offer the potential benefits of environmental adaptation and enhanced nutritional traits, especially in changing climate. Major crops have benefited from genomics-based breeding, initially using single genomes later pangenomes. Recent advances DNA sequencing enabled pangenome construction for several offering more comprehensive understanding genetic diversity. Orphan crop research has now entered pangenomics era applying these pangenomes with advanced selection methods genome editing technologies can transform neglected species into broader agricultural significance. A reference is not enough to harness full variation species. Here, authors review principles, applications improvement, discuss possible ways support pangenomics-driven molecular breeding.

Язык: Английский

Процитировано

1
Comparative Transcriptomic Analysis Reveals Domestication and Improvement Patterns of Broomcorn Millet (Panicum miliaceum L.) DOI Open Access
Xinyu Zhao, Minxuan Liu, Chunxiang Li

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(20), С. 11012 - 11012

Опубликована: Окт. 13, 2024

Broomcorn millet (

Язык: Английский

Процитировано

0
An optimized protocol for in vitro regeneration and genetic transformation of broomcorn millet DOI Creative Commons

Zhaolan Cui,

Wenmin Wei,

Xinqi Han

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Авг. 2, 2024

Abstract Background Broomcorn millet has many advantages, such as abiotic stress resistance, a short growth cycle and high nutritional value. However, due to the lack of efficient genetic transformation methods for broomcorn millet, characterization genes related important traits lags behind that other crop species. Therefore, establishing in vitro regeneration is essential. Results In this study, we used mature seeds from sequenced cultivar 'Longmi 4' explants optimized their methods. The optimal hormone concentrations embryogenic callus induction medium were 2.5 mg/L 2,4-D 0.5 BAP. shoot media 2 kinetin a-naphthaleneacetic acid. Additionally, cocultivation time was 3 days, hygromcin concentration putative transgenic selection 45 mg/L. efficiency 21.25% after our modification approach. Conclusions Here, present simple highly Agrobacterium-mediated protocol millet. Our work provides tool traits, well new strategy breeding.

Язык: Английский

Процитировано

0