The Plant Journal, Год журнала: 2025, Номер 121(4)

Опубликована: Фев. 1, 2025

In this study, we produced a haplotype-phased genome sequence of the fig tree (Ficus carica L.), non-Rosaceae fruit model species, providing systematic overview organization heterozygous diploid and, for first time in tree, evidence allelic expression direction-shifting among haplotypes. The was used whole analysis heterozygosis, cytosine methylation, and profiles peel pulp tissues. two pseudo-haplotypes spanned approximately 355 346 Mbp, respectively, 97% sequences were associated with 13 chromosome pairs tree. Overall, methylation profile tissues showed no variations between homologous chromosomes. However, detected differential DNA within defined gene regions, particularly upstream regions. Among 6768 coding identified, 4024 exhibited allele-specific expression, 18% specific to 14% pulp. Specifically, 2715 genes consistent, one allele always more expressed than other both On contrary, 22 switched tissues, indicating overdominance suggesting that can express alleles higher or lower depending on developmental environmental triggers. Notably, these key biological processes, including maturation regulation, seed maturation, stress response, highlighting their potential role plant's adaptive functions view editing-based breeding.

Язык: Английский