GFAPβ and GFAPδ Isoforms Expression in Mesenchymal Stem Cells, MSCs Differentiated Towards Schwann-like, and Olfactory Ensheathing Cells
Current Issues in Molecular Biology,
Год журнала:
2025,
Номер
47(1), С. 35 - 35
Опубликована: Янв. 9, 2025
Olfactory
ensheathing
cells
(OECs)
and
mesenchymal
stem
(MSCs)
differentiated
towards
Schwann-like
have
plasticity
properties.
These
express
the
Glial
fibrillary
acidic
protein
(GFAP),
a
type
of
cytoskeletal
that
significantly
regulates
many
cellular
functions,
including
those
promote
needed
for
regeneration.
However,
expression
GFAP
isoforms
(α,
β,
δ)
in
these
has
not
been
characterized.
We
evaluated
by
Polymerase
Chain
Reaction
(PCR)
assay
three
conditions:
(1)
OECs,
(2)
exposed
to
OECs-conditioned
medium
(dBM-MSCs),
(3)
MSC
cell
culture
from
rat
bone
marrow
undifferentiated
(uBM-MSCs).
First,
characterization
phenotyping
was
verified
morphology
immunocytochemistry,
using
p75,
CD90,
antibodies.
Then,
we
found
conditions;
GFAPα
(10.95%AUC)
GFAPβ
(9.17%AUC)
predominantly
followed
dBM-MSCs
(α:
3.99%AUC,
β:
5.66%AUC)
uBM-MSCs
2.47%AUC,
2.97%AUC).
GFAPδ
isoform
similar
groups
(OEC:
9.21%AUC,
dBM-MSCs:
11.10%AUC,
uBM-MSCs:
9.21%AUC).
findings
suggest
different
may
regulate
properties,
potentially
contributing
tissue
remodeling
processes
dBM-MSCs,
uBM-MSCs.
Язык: Английский
Fluorescence ‘turn-on’ sensing of glial fibrillary acidic protein using graphene oxide-quenched copper nanoclusters
Geneva Indongo,
Merin K. Abraham,
Greeshma Rajeevan
и другие.
Microchimica Acta,
Год журнала:
2025,
Номер
192(4)
Опубликована: Март 26, 2025
Язык: Английский
Alzheimer’s Disease, Obesity, and Type 2 Diabetes: Focus on Common Neuroglial Dysfunctions (Critical Review and New Data on Human Brain and Models)
Brain Sciences,
Год журнала:
2024,
Номер
14(11), С. 1101 - 1101
Опубликована: Окт. 30, 2024
Obesity,
type
2
diabetes
(T2D),
and
Alzheimer's
disease
(AD)
are
pathologies
that
affect
millions
of
people
worldwide.
They
have
no
effective
therapy
difficult
to
prevent
control
when
they
develop.
It
has
been
known
for
many
years
these
diseases
pathogenic
aspects
in
common.
We
highlight
this
review
neuroglial
cells
(astroglia,
oligodendroglia,
microglia)
play
a
vital
role
the
origin,
clinical-pathological
development,
course
brain
neurodegeneration.
Moreover,
we
include
new
results
T2D-AD
mouse
model
(APP+PS1
mice
on
high-calorie
diet)
investigating.
Язык: Английский