Transcriptomic Profile Analysis of Brain Tissue in the Absence of Functional TRPM8 Calcium Channel DOI Creative Commons
Erick Benjamin Saldes,

Alexandra Erdmier,

Jai Velpula

и другие.

Biomedicines, Год журнала: 2024, Номер 13(1), С. 75 - 75

Опубликована: Дек. 31, 2024

Background/Objectives: Transient Receptor Potential Melastatin 8 (TRPM8) is a non-selective, Ca2+-permeable cation channel involved in thermoregulation and other physiological processes, such as basal tear secretion, cell differentiation, insulin homeostasis. The activation deactivation of TRPM8 occur through genetic modifications, interactions, signaling cascades. Recent evidence suggests significant role the hypothalamus amygdala related to pain sensation sexual behavior. Notably, has been implicated neuropathic pain, migraines, neurodegenerative diseases Parkinson’s disease. Our laboratory identified testosterone high-affinity ligand TRPM8. deficiency appears influence behavioral traits mice, like increased aggression deficits satiety. Here, we aim explore pathways altered brain tissues TRPM8-deficient mice using expression methylation profiles messenger RNA (mRNA) long non-coding (lncRNA). Specifically, focused on regions integral hormonal control, including olfactory bulb, hypothalamus, amygdala, insula. Methods: was isolated purified for microarray analysis collected from male wild-type knockout mice. Results: We various differentially expressed genes tied multiple pathways. Among them, androgen–estrogen receptor (AR-ER) pathway, steroidogenesis reward cocaine pathway are particularly worth noting. Conclusions: These results should bridge existing gaps knowledge regarding inform potential targets future studies elucidate its behavior changes pathology associated with activity.

Язык: Английский

Glutamatergic heterogeneity in the neuropeptide projections from the lateral hypothalamus to the mouse olfactory bulb DOI Creative Commons
Meizhu Qi,

J. Won,

Claudia Rodríguez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 16, 2025

The direct pathway from the lateral hypothalamus to mouse olfactory bulb (OB) includes neurons that express neuropeptide orexin-A, and others do not. OB-projecting not orexin-A are present in an area of known contain melanin-concentrating hormone (MCH). We used virally mediated anterograde tract tracing immunohistochemistry for MCH demonstrate OB is broadly innervated by axon projections both populations neurons. Orexin-A were expressed each layer across its anterior posterior axis. Both genetically heterogeneous, with subsets co-express isoform vesicular glutamate transporter (VGLUT). high-resolution confocal imaging test whether reflect this glutamatergic heterogeneity. majority (∼57%) putative terminals overlapped VGLUT2, smaller proportions co-expressed VGLUT1, or did overlap either VGLUT1 VGLUT2. In contrast, only ∼26% overlapping VGLUT. Therefore, heterogeneous include can release two different neuropeptides. themselves distinct ratios non-glutamatergic terminals. A projection orexin-A-expressing was confirmed using tracing.Orexin-A (MCH), neuropeptides primarily produced throughout OB.Approximately 64% type 1 (VGLUT1) 2 (VGLUT2). 25%

Язык: Английский

Процитировано

0
Engineered nasal bacteria: A new weapon against obesity? DOI
Cristina Miralpeix, Daniela Cota

Cell Host & Microbe, Год журнала: 2025, Номер 33(4), С. 467 - 469

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Ferroptosis effects and behavioral changes associated with high accumulation and low elimination percentage of silver in brain following a 28-day nasal instillation of silver nanoparticles in mice DOI
Shuyan Niu, Meng-Hao Guo, Haitao Yang

и другие.

Food and Chemical Toxicology, Год журнала: 2025, Номер 202, С. 115567 - 115567

Опубликована: Май 21, 2025

Язык: Английский

Процитировано

0
Hunger signalling in the olfactory bulb primes exploration, food-seeking and peripheral metabolism DOI Creative Commons
Romana Stark, Harry Dempsey, Elizabeth A. Kleeman

и другие.

Molecular Metabolism, Год журнала: 2024, Номер 89, С. 102025 - 102025

Опубликована: Сен. 3, 2024

Although the metabolic state of an organism affects olfactory function, precise mechanisms and their impact on behavior metabolism remain unknown. Here, we assess whether ghrelin receptors (GHSRs) in bulb (OB) increase function influence foraging behaviors metabolism. We performed a detailed behavioural analysis mice lacking GHSRs OB (OBGHSR deletion). also analsyed scRNA-seq spatial transcriptomic datasets to GHSR+ cells main accessory bulbs, as well anterior nucleus. OBGHSR deletion affected discrimination habituation both food non-food odors. Anxiety-like depression-like were significantly greater after deletion, whereas exploratory was reduced, with greatest effect under fasted conditions. impacted feeding evidenced by altered bout number duration, buried food-seeking. increased body weight fat mass, spared utilisation chow diet impaired glucose indicating dysfunction. Cross referenced revealed glutamate neurons Ablation reduced ghrelin-induced finding phenocopied results seen deletion. OBGHSRs help maintain particularly during hunger, facilitate behavioral adaptations that optimise food-seeking anxiogenic environments, priming pathways preparation for consumption.

Язык: Английский

Процитировано

2
Novel Compounds as Orexin Receptor Agonists for Treating Sleep Disorders, Namely, Narcolepsy and Hypersomnia DOI Creative Commons
Ram W. Sabnis

ACS Medicinal Chemistry Letters, Год журнала: 2024, Номер 15(8), С. 1178 - 1179

Опубликована: Июль 3, 2024

Provided herein are novel compounds as orexin receptor agonists, pharmaceutical compositions, use of such in treating sleep disorders, namely, narcolepsy and hypersomnia, processes for preparing compounds.

Язык: Английский

Процитировано

1
Lack of Single Amino Acids Transcriptionally Remodels Sensory Systems to Enhance the Intake of Protein and Microbiota DOI Creative Commons
Gili Ezra-Nevo, Sílvia F. Henriques,

Daniel Muench

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 24, 2024

Adequate intake of dietary essential amino acids (eAAs) is vital for protein synthesis and metabolism. Any single eAA deprivation sufficient to increase in Drosophila melanogaster. How such nutritional -needs- are transformed into behavioral -wants- remains poorly understood. We derived transcriptomes from the heads flies deprived individual eAAs identify mechanisms by which this achieved. found that, while specific deprivations have unique effects on gene expression, a large set changes shared across deprivations. show that Or92a upregulation upon increases exploitation yeast, main source flies. Furthermore, Ir76a was crucial feeding Lactobacillus, gut bacterium ameliorates fitness eAA-deprived Our work uncovers common transcriptional induced an animal reveals novel underlying organisms physiological response challenges.

Язык: Английский

Процитировано

0
Vasopressin differentially modulates the excitability of rat olfactory bulb neuron subtypes DOI Creative Commons
Hajime Suyama, Gaia Bianchini, Michael Lukas

и другие.

Frontiers in Neural Circuits, Год журнала: 2024, Номер 18

Опубликована: Авг. 29, 2024

Vasopressin (VP) plays a crucial role in social memory even at the level of olfactory bulb (OB), where OB VP cells are activated during interactions. However, it remains unclear how modulates processing to enable enhanced discrimination very similar odors, e.g., rat body odors. Thus far, has been shown that reduces firing rates mitral (MCs) odor presentation vivo and decreases amplitudes nerve-evoked excitatory postsynaptic potentials (ON-evoked EPSPs) external tufted vitro . We performed whole-cell patch-clamp recordings population Ca 2+ imaging on acute slices. recorded ON-evoked EPSPs as well spontaneous inhibitory currents (IPSCs) from two types projection neurons: middle (mTCs) MCs. bath application reduced frequencies IPSCs mTCs but did not change those Therefore, we analyzed interneurons, i.e., periglomerular (PGCs) granule (GCs), search for origin increased inhibition mTCs. increase evoked either type interneurons. next two-photon glomerular layer superficial GC responses stronger ON stimulation than experiments should evoke action measured cells. observed influx juxtaglomerular somata. Thus, our findings indicate by neurons via strong input-mediated interneuron activity. This neural modulation could improve representation hence, better discriminability conspecific

Язык: Английский

Процитировано

0
As a phenomenon: Ramadan fasting improves olfactory performance DOI Creative Commons
Doğan Çakan, Hüseyin Baki Yılmaz,

Harun Cansız

и другие.

Laryngoscope Investigative Otolaryngology, Год журнала: 2024, Номер 9(5)

Опубликована: Сен. 23, 2024

The present study objectived to investigate the influence of Ramadan fasting (RF) on olfactory function.

Язык: Английский

Процитировано

0
Transcriptomic Profile Analysis of Brain Tissue in the Absence of Functional TRPM8 Calcium Channel DOI Creative Commons
Erick Benjamin Saldes,

Alexandra Erdmier,

Jai Velpula

и другие.

Biomedicines, Год журнала: 2024, Номер 13(1), С. 75 - 75

Опубликована: Дек. 31, 2024

Background/Objectives: Transient Receptor Potential Melastatin 8 (TRPM8) is a non-selective, Ca2+-permeable cation channel involved in thermoregulation and other physiological processes, such as basal tear secretion, cell differentiation, insulin homeostasis. The activation deactivation of TRPM8 occur through genetic modifications, interactions, signaling cascades. Recent evidence suggests significant role the hypothalamus amygdala related to pain sensation sexual behavior. Notably, has been implicated neuropathic pain, migraines, neurodegenerative diseases Parkinson’s disease. Our laboratory identified testosterone high-affinity ligand TRPM8. deficiency appears influence behavioral traits mice, like increased aggression deficits satiety. Here, we aim explore pathways altered brain tissues TRPM8-deficient mice using expression methylation profiles messenger RNA (mRNA) long non-coding (lncRNA). Specifically, focused on regions integral hormonal control, including olfactory bulb, hypothalamus, amygdala, insula. Methods: was isolated purified for microarray analysis collected from male wild-type knockout mice. Results: We various differentially expressed genes tied multiple pathways. Among them, androgen–estrogen receptor (AR-ER) pathway, steroidogenesis reward cocaine pathway are particularly worth noting. Conclusions: These results should bridge existing gaps knowledge regarding inform potential targets future studies elucidate its behavior changes pathology associated with activity.

Язык: Английский

Процитировано

0