Poly-D,L-Lactic Acid Filler Increases Extracellular Matrix by Modulating Macrophages and Adipose-Derived Stem Cells in Aged Animal Skin

Antioxidants, Год журнала: 2023, Номер 12(6), С. 1204 - 1204

Опубликована: Июнь 1, 2023

Poly-D,L-lactic acid (PDLLA) filler corrects soft tissue volume loss by increasing collagen synthesis in the dermis; however, mechanism is not fully understood. Adipose-derived stem cells (ASCs) are known to attenuate decrease fibroblast that occurs during aging, and nuclear factor (erythroid-derived 2)-like-2 (NRF2) increases ASCs survival inducing M2 macrophage polarization IL-10 expression. We evaluated ability of PDLLA induce fibroblasts modulating macrophages a H2O2-induced cellular senescence model aged animal skin. increased NRF2 expression senescence-induced macrophages. Conditioned media from senescent treated with (PDLLA-CMMΦ) reduced proliferation transforming growth factor-β (TGF-β) (FGF) 2 ASCs. PDLLA-CMMΦ (PDLLA-CMASCs) 1a1 3a1 NF-κB MMP2/3/9 fibroblasts. Injection skin resulted NRF2, IL-10, 1a1, These results suggest increase expression, which stimulates secretion TGF-β FGF2. This leads synthesis, can aging-induced loss.

Язык: Английский

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Human beta defensin-2 loaded PLGA nanoparticles impregnated in collagen-chitosan composite scaffold for the management of diabetic wounds

Biomedicine & Pharmacotherapy, Год журнала: 2023, Номер 161, С. 114540 - 114540

Опубликована: Март 17, 2023

Diabetic wound (DW) is the most devastating complication resulting in significant mortality and morbidity diabetic patients. The standard treatment of DW care fails to address prerequisites treating owing its multifactorial pathophysiology. Henceforth, developing a single strategy handle all loopholes may effectively manage DW. objective current study was formulate Human beta defensin-2 (HBD-2) loaded Poly (lactic-co-glycolic acid) (PLGA) nanoparticle impregnated collagen/chitosan (COL-CS) composite scaffolds for accelerated healing Upon investigation, developed biodegradable crosslinked scaffold possesses low matrix degradation, optimum porosity, sustained drug release than non-crosslinked scaffold. In vitro studies revealed that HBD-2 COL-CS biocompatible cell migration angiogenesis. showed antimicrobial activity S. aureus, E. coli, P. aeruginosa. vivo treated group compared those control groups. ELISA results indicated decrease MMP-9, TNF-α, MPO, NAG, NO with an increase IL-10 group. might be due synergistic effects PLGA (collagen synthesis deposition positive angiogenic effect), (anti-inflammatory, antibacterial, effect, proliferation, migration), COL (established healer stabilizer) CS (antibacterial, controlled release).

Язык: Английский

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Advancements in Regenerative Medicine for Aesthetic Dermatology: A Comprehensive Review and Future Trends

Cosmetics, Год журнала: 2024, Номер 11(2), С. 49 - 49

Опубликована: Март 31, 2024

The growing interest in maintaining a youthful appearance has encouraged an accelerated development of innovative, minimally invasive aesthetic treatments for facial rejuvenation and regeneration. close correlation between tissue repair, regeneration, aging paved the way application regenerative medicine principles cosmetic dermatology. theoretical substrates applications dermo-aesthetics are plentiful. However, dermatology is emerging field needs more data vivo trials to reach consensus on standardization methods. In this review, we summarize techniques as they apply dermatology, suggesting unexplored fields future directions.

Язык: Английский

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Evaluating Poly‐D,L‐Lactic Acid for Lower Eyelid Rejuvenation: Efficacy and Safety

Journal of Cosmetic Dermatology, Год журнала: 2025, Номер 24(3)

Опубликована: Фев. 28, 2025

Injectable fillers like PDLLA offer a novel approach to addressing lower eyelid rejuvenation. Despite their growing popularity, comprehensive studies on newer products are limited. This study evaluates the efficacy and safety of (Juvelook, VAIM Inc., Seoul, Korea) for treating tear trough deformities in Korean women, aiming provide long-term, minimally invasive solution periorbital A selected group women exhibiting underwent treatment with PDLLA. Patients were assessed pre-treatment followed up at 1 week, month, 6 months post-treatment, outcomes measured by independent clinicians using Hirmand classification patient satisfaction scores. demonstrated significant improvements high minimal adverse effects. Follow-up results showed sustained no severe complications, indicating safe profile The highlights PDLLA's potential advantages over traditional fillers, including longer-lasting effects enhanced collagen production, suggesting it as superior option those seeking durable aesthetic improvements. is an effective rejuvenation providing lasting benefits satisfaction, warranting further comparative broader applications.

Язык: Английский

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Unveiling the Mechanism: Injectable Poly‐L‐Lactic Acid's Evolving Role—Insights From Recent Studies

Journal of Cosmetic Dermatology, Год журнала: 2024, Номер unknown

Опубликована: Окт. 16, 2024

For over 25 years, Sculptra poly-L-lactic acid—(PLLA-SCA; Galderma Sweden) is used in aesthetic dermatology, addressing volume loss, skin laxity, and wrinkles effectively [1]. This synthetic, bio-compatible product available as a lyophilized powder with PLLA-SCA microparticles, mannitol, sodium carboxymethylcellulose, prepared suspension using sterile water for injection [2]. Biocompatible, polymeric biomaterials like PLLA, when implanted, induce foreign body reaction shaped by the material's characteristics, patient specifics, implantation technique. Controlling these factors ensures predictable host response, crucial achieving desired outcomes collagen stimulation treatments. While specific molecular mechanisms of PLLA's production are not fully understood, this article reviews literature on PLLA-induced biochemical pathways fibroblasts, adipocytes, macrophages, exploring their interplay. Variations among injectable PLLA formulations, particularly tissue integration degradation rates, noteworthy [3, 4]. differentiates between other formulation. Fibroblasts, known involvement assembly maintenance extracellular matrix (ECM), have been recognized key players mechanism action. In 2012 Courderot-Masuyer et al. [5] presented first evidence that addition 0.1% to growth medium ex vivo human derived from female wrinkles, leads an increase Type I production. Although initial study limited sample size three donors, its findings supported subsequent research. Goldberg [6] substantiated idea PLLA-SCA-induced neogenesis humans. clinical involving 14 subjects, they demonstrated subdermal application significantly enhances Notably, levels increased 65.5% after 3 months, slight decrease at 6-month mark, illustrating sustained effect. Kim [7] explored whether could trigger synthesis fibroblasts. Cultured fibroblasts (Cell line Hs68) showed significant RNA procollagen cell extracts, 48-h exposure PLLA-SCA. Two independent studies results. Huth [8] reported thickening model PLLA-SCA-application, Zhu [9] confirmed increasing concentrations (0.1%, 0.5%, 1%) resulted gradual COL1A1 (collagen 1 alpha 1) COL1A2 mRNA, well protein same line. These also highlighted PLLA-SCA's broader anabolic effects ECM, evidenced similar results elastin—a crosslinking fibers—and changes TIMP-1 -2 (tissue inhibitor metalloprotease) mRNA dose-dependent reduction MMP-1 (matrix metalloprotease levels. linked TGF-β (transforming factor beta) signaling pathway. Increased TGF-β1, phosphorylated SMAD-1 proteins were detected 0.5% align previous research, indicating TGF-β1 auto- paracrine stimuli regulate various fibroblast functions, including proliferation, migration, connective synthesis, wound healing, documented Ashcroft, Yao, Lee [10-12]. [13] provided additional link 3D models. The current mechanistic observation posits following administration suspension, immediate volumization observed, persisting several days until carrier solution absorbed, detailed Moyle's 2004 research [14]. remaining particles incite subclinical inflammatory reaction, described Junge's [15]. entails recruitment monocytes differentiation into which coalesce giant cells, encapsulating bodies composed later Mazzuco [6, 16]. However, alongside multinucleated histiocytes surrounding particles, Goldberg's team presence lymphocytes around superficial dermal vessels some samples. was characterized mild even 6 months post-injection proportion patients (10 out 14). researchers noted occurred without notable suggesting more complex mechanism. 2023, Oh [17] foundation elegant hypothesis coexistence leukocytes absence overt attributing it polarization macrophages. Macrophages exist two subtypes: pro-inflammatory M1 anti-inflammatory, tissue-regenerating M2 macrophages [18, 19]. activated bacterial lipopolysaccharide interferon gamma secrete proinflammatory cytokines (e.g., IL-1, IL-6, IL-12, IL-23). contrast, IL-4 IL-13, promote anti-inflammatory responses regeneration, secreting TGF-β. Using murine models, cultured (RAW264.7), (CCD-986sk), own formulation, observed IL-13 post-PLLA treatment mg/mL), shift toward macrophage both vitro. Although, compared differ time points models (Oh: 48 h culture 28d mice vs. Goldberg: 3, 6, 12 humans) one possible conclusion likely polarized correlating inflammation. substantiate result demonstrating CD-163+ (cluster differentiation-163, marker polarization) [13]. Furthermore, replicated findings, upregulation TGF-β, COL1A1, TIMP1, SMAD2 phosphorylation, downregulation MMP2 MMP3 biopsies. They phosphorylation AKT senescent aged mouse skin, echoing [7]. As side note, already cultures display (MIP1a 1b) incubation 24 [20]. no inflammation markers regulated, finding reflects short-term stress response. Finally, although less play role context PLLA-based Focusing scaffolds post-mastectomy treatments Ogino [21-23] shown consistent fat mass across different animal rats, rabbits, pigs. adipocyte rabbits porous capsule implants under inguinal fat, added [22]. After 12- 24-month invasion implants. sponge surrounded PLLA-mesh, authors very low number while stronger than implant alone. porcine model, achieved: decreased adipose tissue, especially 9 then reducing [23]. contained fibers, notably, capillary formation threads direct process. Recently Jin induced browning primary fibroblast, but adipogenic mesenchymal stem cells [24]. increases lactate uptake via transporter Mct1/4 responsible browning. Contradictory study, effect adipogenesis pre-adipocytes (3T3-L1 cells) post UVB-irradiation, simulating deep photoaging [25]. Day 7 post-differentiation, enhancing Types IV VIɑ1 production, UVB damage. interesting has [26] (as [27] produced upon PLLA-SCA-stimulation). differences due chosen vitro summary, recent advanced our understanding biological effects. encapsulated triggering activation thus promoting new ECM formation. vital role, undergoing PLLA-SCA, inflammation, releasing signals IL-13. stimulates activity Additionally, triggers adipogenesis, may explain treated areas [28]. prompts VI Collagen creating feedback loop regulation. there hints positively affecting [29] Figure 1. Despite progress, questions persist. precise recognition remains unclear. Further needed immunological angiogenesis, interaction cells. Luiz Eduardo Avelar investigator, speaker, consultant Galderma. Schafiq Nabhani employee Stas Wüst former submission fees process funded data support openly PubMed (National Library Medicine, NLM) https://pubmed.ncbi.nlm.nih.gov/.

Язык: Английский

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Injectable Poly-l-Lactic Acid for Body Aesthetic Treatments: An International Consensus on Evidence Assessment and Practical Recommendations

Aesthetic Plastic Surgery, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 26, 2024

Abstract Non-facial (body) rejuvenation is a treatment of increasing demand, with patients seeking to harmonize the benefits rejuvenated facial appearance other areas body. Poly- l -lactic acid (PLLA)-SCA (Sculptra®) has been approved for aesthetic uses since 1999 in Europe and 2009 USA more recently evaluated cellulite buttocks thighs body indications. The current evidence base consists largely prospective observational analyses case series although systematic evaluations number are underway. Current data support potential benefit use PLLA-SCA neck, knees, abdomen, hands upper arms. Improvements (e.g. sagging, wrinkling, dimpling, cellulite) that sustained over time minimal side effects can be achieved. Standardization injection protocols different now needed along validation clinical tools used agree on goals evaluate improvements time. A group international experts fields aesthetics, plastic surgery dermatology were selected based their educational, scientific publication merits together experience using rejuvenation. Here, we provide an evidence-based expert-led consensus (14 years off-face experience) recommendations appropriate sites evaluation when biostimulator procedures. Level Evidence IV This journal requires authors assign level each article. For full description these Evidence-Based Medicine ratings, please refer Table Contents or online Instructions Authors www.springer.com/00266 .

Язык: Английский

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Octanoyl esterification of low molecular weight sulfated galactan enhances the cellular uptake and collagen expression in fibroblast cells

Biomedical Reports, Год журнала: 2023, Номер 19(6)

Опубликована: Окт. 23, 2023

Low molecular weight sulfated galactan (LMSG) supplemented with octanoyl ester (Oct-LMSG) demonstrated superior wound healing activity compared to the unsupplemented LMSG in a fibroblast model. To test hypothesis that increased bioactivity of Oct-LMSG may depend on its penetration into plasma membrane, cellular uptake was investigated and collagen production cells assessed for first time. The examined using indirect immunofluorescence confocal laser scanning microscope. In addition, degree activation associated this evaluated. results indicated internalization fibroblasts treated Oct-LMSG. Transmission electron micrographs revealed ultrastructure active protein upon treatment upregulated expression type I mRNA proteins, as well related signaling molecules involved synthesis, including α1 chain (Col1A1), Col1A2, phosphorylated (p)-Smad2/3 p-Smad4. current findings support notion supplementation enhanced and, result, regulated via Smad pathway. This study demonstrates therapeutic potential promoting tissue regeneration.

Язык: Английский

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Dermal fibroblasts in the focus of modern cosmetology: aging and response to cosmetic procedures: A review

Consilium Medicum, Год журнала: 2024, Номер 26(8), С. 541 - 549

Опубликована: Окт. 6, 2024

The article presents a review of modern information about the structure and function dermal fibroblasts. morphophysiology fibroblasts, their role in formation connective tissue dermis its restoration after damage are discussed. Detailed knowledge proliferative synthetic activity fibroblasts seems to be very important reasonable choice methods for correction age-related skin changes sequence invasive procedures.

Язык: Английский

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Heparan sulfate 6-O-sulfotransferase 2 promotes gastric cancer progression by modulating the TGF-β/smad2/3 pathway

American Journal of Translational Research, Год журнала: 2024, Номер 16(12), С. 7924 - 7936

Опубликована: Янв. 1, 2024

To investigate the role of heparan sulfate 6-O-sulfotransferase 2 (HS6ST2) in gastric cancer (GC). HS6ST2 expression GC and adjacent normal mucosa was first detected via immunohistochemical (IHC) staining. The correlation between level clinicopathological parameters were observed. protein AGS, MKN45 GES-1 cells examined using Western blotting. function explored CCK-8, wound healing Transwell assays. elucidate underlying molecular mechanisms, we whether modulated TGF-β/smad2/3 signaling pathway. Finally, investigated tumor growth a nude mouse model. tissues significantly higher than that positively correlated with size. Compared cells, AGS elevated. Silencing impaired cell growth, mobility epithelial-mesenchymal transition (EMT). On other hand, upregulation increased migration invasion, which dramatically blocked by SB431542 treatment. Furthermore, xenograft experiments demonstrated silencing inhibited EMT vivo. promotes progression through modulation

Язык: Английский

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Enhancement of skin regeneration through activation of TGF-β/SMAD signaling pathway by Panax ginseng meyer non-edible callus-derived extracellular vesicles

Journal of Ginseng Research, Год журнала: 2024, Номер 49(1), С. 34 - 41

Опубликована: Авг. 24, 2024

This study aimed to investigate the effects of ginseng non-edible callus-derived extracellular vesicle (GNEV) on skin regeneration, particularly focusing its impact proliferation and migration in human dermal fibroblast (HDF). GNEV was isolated from callus using sequential filtration size exclusion chromatography (SEC). The characterized nanoparticle tracking analysis (NTA). HDF treated with various concentrations GNEV, cell viability, proliferation, were assessed MTT scratch wound healing assays. Gene expression related collagen synthesis (TGF-β, SMAD-2, SMAD-3, COL1A1) measured RT-PCR. Treatment resulted a significant 2.5-fold increase compared non-treated group. Furthermore, demonstrated upregulation genes, specifically TGF-β, COL1A1, by 41.7 %, 59.4 60.2 21.8 respectively. These findings indicated that activates TGF-β/SMAD signaling pathway, showcasing potential induce regeneration. In conclusion, exhibits notable ability enhance regeneration through stimulatory key genes. activation pathway further suggests as promising candidate for drug delivery systems fields cosmetics pharmaceuticals, opening avenues research application skincare dermatology.

Язык: Английский

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