Exosomal miR‐155‐5p Facilitates Lipopolysaccharide Transport and Foam Cell Formation: A Novel Link Between Periodontitis and Atherosclerosis DOI Open Access

Wenwen Yang,

Qingxiang Li, Fei Wang

и другие.

Journal of Periodontal Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

ABSTRACT Aim To investigate the role of lipopolysaccharide (LPS) from Porphyromonas gingivalis and miR‐155‐5p‐enriched exosomes in formation foam cells occurrence carotid atherosclerosis (CAS). Methods The CAS tissue samples plasma healthy control group or patients undergoing periodontitis without with were collected at Xuanwu Hospital, Capital Medical University. expression level miR‐155‐5p was evaluated by immunofluorescent analysis qRT‐PCR. Oil red O staining lipid accumulation assays performed to explore effects LPS on mouse macrophage Raw264.7 human monocytes THP‐1. levels lipid‐regulated genes detected Dual‐luciferase reporter gene assay DET1 overexpressed inhibited used verify target exosomal miR‐155‐5p. ApoE −/− mice confirm auxo‐action vivo, LAL detect content. Results higher than those CAS. significantly increased tissues compared Normal tissues, associated tissues. MiR‐155‐5p‐enriched accelerated macrophage‐like promoted activity lipid‐accumulation targeting . In mice, circulating captured LPS, LPS‐laden conferred access for triggering Conclusion enriched capture escort atherosclerotic sites, licensing thus promoting

Язык: Английский

Exosomes in Oral Diseases: Mechanisms and Therapeutic Applications DOI Creative Commons
Qing Miao, Shaoqing Li, Wei Lyu

и другие.

Drug Design Development and Therapy, Год журнала: 2025, Номер Volume 19, С. 457 - 469

Опубликована: Янв. 1, 2025

Exosomes, small extracellular vesicles secreted by various cells, play crucial roles in the pathogenesis and treatment of oral diseases. Recent studies have highlighted their involvement orthodontics, periodontitis, squamous cell carcinoma (OSCC), hand, foot, mouth disease (HFMD). Exosomes a positive effect on inflammatory environment cavity, remodeling regeneration tissues, offer promising therapeutic options for bone periodontal tissue restoration. In OSCC tumor-derived exosomes promote cancer progression through proliferation, migration, invasion, angiogenesis, serve as potential biomarkers early diagnosis prognosis. Additionally, engineered constructed specifically based exosome properties hold great promise targeted drug delivery regenerative therapies such orthodontics healing. With continued research, improving diseases, advancing personalized therapies.

Язык: Английский

Процитировано

0

Extracellular Vesicles in Periodontitis: Pathogenic Mechanisms and Therapeutic Potential DOI Creative Commons
Ling Zhang, Xiaotong Li,

Bin Zhang

и другие.

Journal of Inflammation Research, Год журнала: 2025, Номер Volume 18, С. 1317 - 1331

Опубликована: Янв. 1, 2025

Periodontitis is a prevalent yet frequently overlooked oral disease that linked to range of systemic conditions. Although basic treatment and periodontal surgery can alleviate the symptoms periodontitis certain extent, severe tissue defects or refractory cases not effective. Extracellular vesicles (EVs) are subcellular lipid bilayer particles come from variety sources in biological fluids vertebrates. They play key role intercellular communication by transporting multiple signaling molecules. Recent research has indicated EVs derived pathogens trigger periodontitis, exacerbate damage, potentially disseminate other parts body, leading Conversely, extracellular dental stem cells (DSCs) have demonstrated ability regulate local immune environment foster regeneration repair tissues, positioning them as promising candidate for cell-free therapeutic approaches periodontitis. This review aims summarize latest on involvement different pathogenesis especially systematically elucidate mechanism secreted periodontitis-related diseases first time. By uncovering these complex regulatory processes, new more effective be explored battle against its associated diseases.

Язык: Английский

Процитировано

0

Youthful small extracellular vesicles restore the function and reparative capacity of inflammatory-impaired periodontal ligament stem cells via delivering protein biglycan for bone regeneration DOI Creative Commons
Jiaqi Yang,

Junxiang Su,

Zhuo Sun

и другие.

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Авг. 24, 2024

Regenerating inflamed bone defects represents a severe clinical challenge due to the undesirable inflammatory microenvironment. The stimulus poses weighty threat regenerative capacity of endogenously derived mesenchymal stem cells (MSCs), which are mainly responsible for osteogenic differentiation, thereby resulting in compromised endogenous formation. Consequently, alleviating biological characteristics inflammatory-impaired MSCs is crucial promoting regeneration. Nano-sized small extracellular vesicles (sEVs) have emerged as promising therapeutic tools orchestrate fate their intrinsic biocompatibility and encapsulated bioactive contents. In present study, we extracted sEVs from youthful adult dental pulp explored ability recover inflammation-compromised periodontal ligament (IPDLSCs). results indicated that both types were capable facilitating IPDLSCs osteogenesis. However, young exhibited more robust potential at lower concentration compared sEVs. Mechanically, enhanced expression morphogenetic protein 4 (BMP4) via delivering Biglycan, correspondingly promoted capability IPDLSCs. Collectively, our findings emphasized hold enormous rescue inherent function competence inflammation-impaired MSCs, shedding light on prospects infected

Язык: Английский

Процитировано

2

Severe Periodontitis is Associated With Recurrent Cardiovascular Events—A 10‐Year Longitudinal Cohort Study DOI Creative Commons
Stefan Reichert,

Selina Rehm,

Axel Schlitt

и другие.

Journal of Periodontal Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 22, 2024

ABSTRACT Aim The study aimed to elucidate a putative association between severe periodontitis and the incidence of recurrent cardiovascular events in patients with disease (CVD) within 10 years after their initial hospitalisation. Methods A cohort 1002 stationary angiographically proven CVD was included. They were examined regarding prevalence (≥ 30% teeth proximal attachment loss ≥ 5 mm), probing depth, clinical loss, bleeding on probing, number missing oral care habits. Recurrent summarised as combined end point (myocardial infarction, stroke/transitory ischemic attack, death caused by stroke). Survival analyses carried out 10‐year follow‐up period. Hazard ratios (HRs) adjusted for known cardiac risk factors using Cox regression. Results completed 792 patients. overall 42.8%. Severe associated (adjusted hazard ratio [HR] = 1.26, 95% confidence interval [CI] 1.0–1.58 Standard error [SE] 0.11), whereas both, tooth brushing more than once day HR 0.74, CI 0.57–0.97, SE 0.13) performing interdental hygiene 0.71, 0.52–0.99, 0.16) decreased this risk. Conclusions is factor events. Trial Registration ClinicalTrials.gov identifier: NCT01045070

Язык: Английский

Процитировано

2

Exosomal miR‐155‐5p Facilitates Lipopolysaccharide Transport and Foam Cell Formation: A Novel Link Between Periodontitis and Atherosclerosis DOI Open Access

Wenwen Yang,

Qingxiang Li, Fei Wang

и другие.

Journal of Periodontal Research, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

ABSTRACT Aim To investigate the role of lipopolysaccharide (LPS) from Porphyromonas gingivalis and miR‐155‐5p‐enriched exosomes in formation foam cells occurrence carotid atherosclerosis (CAS). Methods The CAS tissue samples plasma healthy control group or patients undergoing periodontitis without with were collected at Xuanwu Hospital, Capital Medical University. expression level miR‐155‐5p was evaluated by immunofluorescent analysis qRT‐PCR. Oil red O staining lipid accumulation assays performed to explore effects LPS on mouse macrophage Raw264.7 human monocytes THP‐1. levels lipid‐regulated genes detected Dual‐luciferase reporter gene assay DET1 overexpressed inhibited used verify target exosomal miR‐155‐5p. ApoE −/− mice confirm auxo‐action vivo, LAL detect content. Results higher than those CAS. significantly increased tissues compared Normal tissues, associated tissues. MiR‐155‐5p‐enriched accelerated macrophage‐like promoted activity lipid‐accumulation targeting . In mice, circulating captured LPS, LPS‐laden conferred access for triggering Conclusion enriched capture escort atherosclerotic sites, licensing thus promoting

Язык: Английский

Процитировано

0