An innovative approach for marine macro-organism monitoring: methodology and future perspectives of environmental DNA (eDNA) technology
Marine Biology,
Год журнала:
2025,
Номер
172(3)
Опубликована: Март 1, 2025
Язык: Английский
Tree of life metabarcoding can serve as a biotic benchmark for shifting baselines in urbanized estuaries
Environmental Research,
Год журнала:
2024,
Номер
258, С. 119454 - 119454
Опубликована: Июнь 19, 2024
Urbanization
of
estuaries
drastically
changed
existing
shorelines
and
bathymetric
contours,
in
turn
modifying
habitat
for
marine
foundational
species
that
host
critical
biodiversity.
And
yet
we
lack
approaches
to
characterize
a
significant
fraction
the
biota
inhabit
these
ecosystems
on
time
scales
align
with
rates
urbanization.
Environmental
DNA
(or
eDNA)
metabarcoding
combines
multiple
assays
targeting
broad
range
taxonomic
groups
can
provide
solution,
but
need
determine
whether
biological
communities
it
detects
ally
different
habitats
changing
aquatic
environments.
In
this
study,
tested
tree
life
(ToL-metabarcoding)
data
extracted
from
filtered
seawater
samples
correlated
four
known
geomorphic
zones
across
heavily
urbanized
estuary
(Sydney
Harbour,
Australia).
Using
method,
substantially
expanded
our
knowledge
composition
spatial
distribution
biodiversity
Sydney
particularly
organisms
where
records
are
sparse.
Excluding
terrestrial
inputs,
identified
effects
both
distance
mouth
Harbour
zone
community
structure
ToL-metabarcoding
dataset
(entire
community),
as
well
each
subgroups
considered
(fish,
macroinvertebrates,
algae
plants,
bacteria).
This
effect
appeared
be
driven
by
taxa
collective
versus
few
individual
taxa,
taxon
explaining
no
more
than
0.62%
variation
between
zones.
Similarly,
richness
was
significantly
higher
within
large
sample
sizes,
also
decreased
1%
additional
kilometer
mouth,
result
consistent
reduction
tidal
inputs
available
upper
catchments.
Based
results,
suggest
used
benchmark
monitoring
other
globally,
at
future
points
based
detection
bioindicators
life.
We
robust
biotic
snapshots
archived
following
extensive
curation
assignments
incorporates
ecological
affinities,
supported
relevant
regional
repositories.
Язык: Английский
Microbial, holobiont, and Tree of Life eDNA/eRNA for enhanced ecological assessment
Trends in Microbiology,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 1, 2024
Microbial
environmental
DNA
and
RNA
(collectively
'eNA')
originate
from
a
diverse
abundant
array
of
microbes
present
in
samples.
These
eNA
signals,
largely
representing
whole
organisms,
serve
as
powerful
complement
to
signals
derived
fragments
or
remnants
larger
organisms.
Integrating
microbial
data
into
the
toolbox
ecosystem
assessments
biotic
indices
therefore
has
potential
transform
how
we
use
understand
biodiversity
dynamics
functions,
inform
next
generation
monitoring.
Incorporating
holobiont
Tree
Life
approaches
analyses
offers
further
holistic
insight
range
ecological
interactions
between
other
paving
way
for
advancing
our
understanding
of,
ultimately
manipulating
properties
pertinent
management,
conservation,
wildlife
health,
food
production.
Язык: Английский