Molecular Ecology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 12, 2025
ABSTRACT Epigenetic changes in the methylation of DNA may occur response to environmental stressors, including warming climates. also play an important role regulating gene expression during both male and female reproduction many insect species. However, it is currently unknown how shifts when individuals are reproducing under warmer temperatures. We exposed European corn borer moths ( Ostrinia nubilalis ) heat pupal adult life stages then investigated across genome using enzymatic methyl‐seq (EM‐seq). compared patterns reproductive males females (28°C) those that experienced ambient temperature (23°C). found exposure led a small but significant increase percentage methylated CpG sites throughout sexes. rates were higher differential following localised unique regions each sex. In males, shifted within genes belonging pathways Hippo signalling, ubiquitin‐mediated proteolysis, damage repair spermatogenesis. females, occurred related histone modification oogenesis. Our results suggest respond moderate Lepidoptera provide insight into epigenetic responses heatwaves, suggesting novel be involved responding stress metamorphosis reproduction.
Язык: Английский