Rhs NADase effectors and their immunity proteins are exchangeable mediators of inter-bacterial competition in Serratia DOI Creative Commons

Martin Hagan,

G. Pankov, Ramsés Gallegos‐Monterrosa

и другие.

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Сен. 28, 2023

Abstract Many bacterial species use Type VI secretion systems (T6SSs) to deliver anti-bacterial effector proteins into neighbouring cells, representing an important mechanism of inter-bacterial competition. Specific immunity protect bacteria from the toxic action their own effectors, whilst orphan without a cognate may provide protection against incoming effectors non-self competitors. T6SS-dependent Rhs contain variable C-terminal toxin domain (CT), with protein encoded immediately downstream effector. Here, we demonstrate that Rhs1 two strains Serratia marcescens , model strain Db10 and clinical isolate SJC1036, possess distinct CTs which both display NAD(P) + glycohydrolase activity but belong different subgroups NADase each other T6SS-associated NADases. Comparative structural analysis identifies conserved functions required for reveals unrelated utilise common inhibition. By replicating natural recombination event, show successful functional exchange encodes provides T6SS-delivered SJC1036 NADase. Our findings highlight flexible during inter-strain competition repeated adoption toxins as weapons cells.

Язык: Английский

Rhizosphere bacterial interactions and impact on plant health DOI Creative Commons

Jane Chepsergon,

Lucy Moleleki

Current Opinion in Microbiology, Год журнала: 2023, Номер 73, С. 102297 - 102297

Опубликована: Март 30, 2023

The rhizosphere is a chemically complex environment that harbors strikingly diverse microbial community. past few decades have seen rapid growth in the body of literature on plant–microbe–microbe interactions and plant health. Thus, aim this paper to review current knowledge (specifically bacteria) how these influence microbiomes impact This article discusses (i) recruits beneficial bacteria ii) competition between mechanisms/weapons employed bacteria–bacteria shapes microbiome turn affects heath. discussion mainly focuses interference competition, characterized by production specialized metabolites (antibacterial compounds) exploitative where bacterial strain restricts competitor's access nutrients such as through secretion siderophores could allude cooperation. Understanding mechanisms plant–bacteria provide insights into manipulate for improved agricultural outcomes.

Язык: Английский

Процитировано

71
The ecological impact of a bacterial weapon: microbial interactions and the Type VI secretion system DOI Creative Commons
Ramsés Gallegos‐Monterrosa, Sarah J. Coulthurst

FEMS Microbiology Reviews, Год журнала: 2021, Номер 45(6)

Опубликована: Июнь 20, 2021

Bacteria inhabit all known ecological niches and establish interactions with organisms from kingdoms of life. These are mediated by a wide variety mechanisms very often involve the secretion diverse molecules bacterial cells. The Type VI system (T6SS) is protein that uses bacteriophage-like machinery to secrete array effectors, usually translocating them directly into neighbouring effectors display toxic activity in recipient cell, making T6SS an effective weapon during inter-bacterial competition eukaryotic Over last two decades, microbiology research has experienced shift towards using systems-based approaches study between their communities context. Here, we focus on this aspect T6SS. We consider how our perspective developed examine what currently about impact bacteria deploying can have environments, including associated plants, insects mammals. T6SS-mediated affect host shaping microbiota, as well be established different microorganisms through deployment versatile system.

Язык: Английский

Процитировано

62
SecReT6 update: a comprehensive resource of bacterial Type VI Secretion Systems DOI
Jianfeng Zhang, Jiahao Guan, Meng Wang

и другие.

Science China Life Sciences, Год журнала: 2022, Номер 66(3), С. 626 - 634

Опубликована: Ноя. 4, 2022

Язык: Английский

Процитировано

41
An ADP-ribosyltransferase toxin kills bacterial cells by modifying structured non-coding RNAs DOI Creative Commons
Nathan P. Bullen, David Sychantha,

Stephanie S. Thang

и другие.

Molecular Cell, Год журнала: 2022, Номер 82(18), С. 3484 - 3498.e11

Опубликована: Сен. 1, 2022

Язык: Английский

Процитировано

38
Pathogenicity and virulence of Acinetobacter baumannii : Factors contributing to the fitness in healthcare settings and the infected host DOI Creative Commons
Massimiliano Lucidi, Daniela Visaggio, Antonella Migliaccio

и другие.

Virulence, Год журнала: 2023, Номер 15(1)

Опубликована: Дек. 6, 2023

Acinetobacter baumannii is a common cause of healthcare-associated infections and hospital outbreaks, particularly in intensive care units. Much the success A. relies on its genomic plasticity, which allows rapid adaptation to adversity stress. The capacity acquire novel antibiotic resistance determinants tolerance stresses encountered environment promote spread among patients long-term contamination healthcare setting. This review explores virulence factors physiological traits contributing infection environment. Several cell-associated secreted involved biofilm formation, cell adhesion, invasion, persistence host, as well xeric stress imposed by settings, are illustrated give reasons for pathogen.

Язык: Английский

Процитировано

37
Killing in the name of: T6SS structure and effector diversity DOI
Luke P. Allsopp, Patricia Bernal

Microbiology, Год журнала: 2023, Номер 169(7)

Опубликована: Июль 25, 2023

The life of bacteria is challenging, to endure employ a range mechanisms optimize their environment, including deploying the type VI secretion system (T6SS). Acting as bacterial crossbow, this delivers effectors responsible for subverting host cells, killing competitors and facilitating general access common goods. Due its importance, lethal machine has been evolutionarily maintained, disseminated specialized fulfil these vital functions. In fact, T6SS structural clusters are present in over 25 % Gram-negative bacteria, varying number from one six different genetic per organism. Since discovery 2006, research on rapidly progressed, yielding remarkable breakthroughs. identification characterization novel components T6SS, combined with biochemical studies, have revealed fascinating governing assembly, loading, firing disassembly processes. Recent findings also demonstrated efficacy against fungal Gram-positive expanding scope. Ongoing continues uncover an extensive repertoire effectors, genuine mediators function. These studies shedding light new aspects biology prokaryotic eukaryotic organisms. This review provides comprehensive overview highlighting recent discoveries structure diversity effectors. Additionally, it injects personal perspective avenues future research, aiming deepen our understanding combative system.

Язык: Английский

Процитировано

35
Blood–Brain Barrier Integrity Damage in Bacterial Meningitis: The Underlying Link, Mechanisms, and Therapeutic Targets DOI Open Access
Ruicheng Yang, Jundan Wang, Fen Wang

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(3), С. 2852 - 2852

Опубликована: Фев. 2, 2023

Despite advances in supportive care and antimicrobial treatment, bacterial meningitis remains the most serious infection of central nervous system (CNS) that poses a risk to life. This clinical dilemma is largely due our insufficient knowledge pathology behind this disease. By controlling entry molecules into CNS microenvironment, blood-brain barrier (BBB), highly selective cellular monolayer specific CNS's microvasculature, regulates communication between rest body. A defining feature pathogenesis increase BBB permeability. So far, several contributing factors for disruption have been reported, including direct damage brought on by virulence factors, as well host-specific proteins or inflammatory pathways being activated. Recent studies demonstrated targeting pathological enhanced permeability an effective therapeutic complement therapy treating meningitis. Hence, understanding how these meningitis-causing pathogens affect will provide novel perspectives investigating meningitis's pathogenesis, prevention, therapies. Here, we summarized recent research progress disrupting function BBB. review provides handy information pathogens, helps design future develop potential combination

Язык: Английский

Процитировано

34
Identification of type VI secretion system effector-immunity pairs using structural bioinformatics DOI Creative Commons
Alexander Martin Geller,

Maor Shalom,

David Zlotkin

и другие.

Molecular Systems Biology, Год журнала: 2024, Номер 20(6), С. 702 - 718

Опубликована: Апрель 24, 2024

Abstract The type VI secretion system (T6SS) is an important mediator of microbe–microbe and microbe–host interactions. Gram-negative bacteria use the T6SS to inject effectors (T6Es), which are usually proteins with toxic activity, into neighboring cells. Antibacterial have cognate immunity that neutralize self-intoxication. Here, we applied novel structural bioinformatic tools perform systematic discovery functional annotation T6Es their from a dataset 17,920 T6SS-encoding bacterial genomes. Using clustering, identified 517 putative T6E families, outperforming sequence-based clustering. We developed logistic regression model reliably quantify protein–protein interaction new T6E-immunity pairs, yielding candidate for 231 out families. used sensitive structure-based yielded annotations 51% again annotation. Next, validated four pairs using basic experiments in E. coli . In particular, showed Pfam domain DUF3289 homolog Colicin M DUF943 acts as its protein. Furthermore, discovered SleB, lytic transglycosylase, specific glutamate catalytic residue. Overall, this study applies pair discovery, provides extensive database annotated pairs.

Язык: Английский

Процитировано

8
Antifungal weapons of Lysobacter , a mighty biocontrol agent DOI Open Access
Long Lin,

Kangwen Xu,

Danyu Shen

и другие.

Environmental Microbiology, Год журнала: 2021, Номер 23(10), С. 5704 - 5715

Опубликована: Июль 19, 2021

Summary Bacteria interact with fungi in a variety of ways to inhibit fungal growth, while the underlying mechanisms remain only partially characterized. The plant‐beneficial Bacillus and Pseudomonas species are well‐known antifungal biocontrol agents, whereas Lysobacter far less studied. Members easy grow fermenters safe humans, animals plants. These environmentally ubiquitous bacteria use diverse arsenal weapons prey on other microorganisms, including oomycetes. small molecular toxins secreted by represent long‐range effective against filamentous fungi. hydrolytic enzymes act as intermediate‐range non‐filamentous contact‐dependent killing devices proposed work short‐range weapons. We describe here structure, biosynthetic pathway, action mode applications one best‐characterized weapons, heat‐stable factor (HSAF) produced enzymogenes . discuss how flagellar type III secretion system has evolved into an enzyme machine for highlight intricate mechanism coordinating production weapon, HSAF device, VI system. also overview regulatory involving specific transcription factors bacterial second messenger c‐di‐GMP.

Язык: Английский

Процитировано

47
Mounting, structure and autocleavage of a type VI secretion-associated Rhs polymorphic toxin DOI Creative Commons
Dukas Jurėnas, Leonardo T. Rosa, Martial Rey

и другие.

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Дек. 1, 2021

Abstract Bacteria have evolved toxins to outcompete other bacteria or hijack host cell pathways. One broad family of bacterial polymorphic gathers multidomain proteins with a modular organization, comprising C-terminal toxin domain fused N-terminal that adapts the delivery apparatus. Polymorphic include bacteriocins, contact-dependent growth inhibition systems, and specialized Hcp, VgrG, PAAR Rhs Type VI secretion (T6SS) components. We recently described characterized Tre23, T6SS-associated protein in Photorhabdus laumondii , Rhs1. Here, we show Rhs1 forms complex T6SS spike VgrG EagR chaperone. Using truncation derivatives cross-linking mass spectrometry, demonstrate VgrG-EagR-Rhs1 formation requires β-helix region. then report cryo-electron-microscopy structure Rhs1-EagR complex, demonstrating central region β-barrel cage-like encapsulates domain, provide evidence for processing through aspartyl autoproteolysis. propose model loading on T6SS, transport into target cell.

Язык: Английский

Процитировано

42