Environmental exposure to perchlorate, nitrate, and thiocyanate in relation to biological aging in U.S. adults, a cross-sectional NHANES study
Frontiers in Public Health,
Год журнала:
2025,
Номер
13
Опубликована: Март 18, 2025
Few
studies
have
investigated
the
associations
between
perchlorate,
nitrate,
and
thiocyanate
(PNT)
biological
aging.
This
study
aimed
to
assess
association
PNT
aging
among
U.S.
adults.
Utilizing
multivariable
linear
regression
restricted
cubic
splines
(RCS),
we
analyzed
urinary
levels'
impact
on
phenotypic
age
age.
Subgroup
sensitivity
analyses
were
also
conducted.
Weighted
Quantile
Sum
(WQS)
Bayesian
Kernel
Machine
Regression
(BKMR)
models
examined
mixtures.
8,368
participants
analyzed.
Mean
was
43.05
±
0.48
years,
mean
47.08
0.4
years.
Multivariable
showed
significant
negative
higher
levels
(perchlorate
β
=
-0.6,
95%
CI:
-0.93
-0.27;
nitrate
-0.81,
-1.19
-0.42;
-0.56,
-0.77
-0.34)
after
covariates
adjusted.
RCS
demonstrated
nonlinear
relationships
exposure
(nonlinear
p
values:
0.002,
<0.001,
<0.001),
with
stable
results
in
analyses.
Nitrate
a
(β
-0.78,
-1.13
-0.44),
indicating
consistent
relationship
observed
through
remaining
across
WQS
revealed
mixture
both
positive
directions,
direction.
BKMR
analysis
mixtures
age,
identified
as
primary
predictors
of
No
found
Individual
or
combined
are
negatively
associated
High
is
reduced
showcasing
outcomes.
Язык: Английский
Exploring Mammalian Heme Peroxidases: A comprehensive review on the structure and function of Myeloperoxidase, Lactoperoxidase, Eosinophil peroxidase, Thyroid peroxidase and Peroxidasin.
Archives of Biochemistry and Biophysics,
Год журнала:
2024,
Номер
unknown, С. 110155 - 110155
Опубликована: Сен. 1, 2024
Язык: Английский
Expression of RcrB confers resistance to hypochlorous acid in uropathogenic Escherichia coli
Journal of Bacteriology,
Год журнала:
2023,
Номер
205(10)
Опубликована: Окт. 4, 2023
ABSTRACT
To
eradicate
bacterial
pathogens,
neutrophils
are
recruited
to
the
sites
of
infection,
where
they
engulf
and
kill
microbes
through
production
reactive
oxygen
chlorine
species
(ROS/RCS).
The
most
prominent
RCS
is
antimicrobial
oxidant
hypochlorous
acid
(HOCl),
which
rapidly
reacts
with
various
amino
side
chains,
including
those
containing
sulfur
primary/tertiary
amines,
causing
significant
macromolecular
damage.
Pathogens
like
uropathogenic
Escherichia
coli
(UPEC),
primary
causative
agent
urinary
tract
infections,
have
developed
sophisticated
defense
systems
protect
themselves
from
HOCl.
We
recently
identified
RcrR
regulon
as
a
novel
HOCl
strategy
in
UPEC.
Expression
rcrARB
operon
controlled
by
HOCl-sensing
transcriptional
repressor
RcrR,
oxidatively
inactivated
resulting
expression
its
target
genes,
rcrB
.
gene
encodes
hypothetical
membrane
protein,
deletion
substantially
increases
UPEC’s
susceptibility
However,
mechanism
behind
protection
RcrB
unclear.
In
this
study,
we
investigated
whether
(i)
mode
action
requires
additional
help,
(ii)
induced
physiologically
relevant
oxidants
other
than
HOCl,
(iii)
system
limited
specific
media
and/or
cultivation
conditions.
provide
evidence
that
sufficient
E.
Furthermore,
protects
several
but
not
ROS.
plays
protective
role
for
RCS-stressed
planktonic
cells
under
growth
conditions
appears
be
irrelevant
biofilm
formation.
IMPORTANCE
Bacterial
infections
pose
an
increasing
threat
human
health,
exacerbating
demand
alternative
treatments.
Uropathogenic
common
etiological
(UTIs),
confronted
neutrophilic
attacks
bladder,
must
therefore
equipped
powerful
fend
off
toxic
effects
species.
How
UPEC
deal
negative
consequences
oxidative
burst
neutrophil
phagosome
remains
Our
study
sheds
light
on
requirements
RcrB,
potent
toward
(HOCl)
stress
phagocytosis.
Thus,
could
potentially
serve
attractive
drug
increase
body’s
own
capacity
fight
UTIs.
Язык: Английский
The neutrophil oxidant hypothiocyanous acid causes a thiol-specific stress response and an oxidative shift of the bacillithiol redox potential in Staphylococcus aureus
Microbiology Spectrum,
Год журнала:
2023,
Номер
11(6)
Опубликована: Ноя. 6, 2023
ABSTRACT
During
infections,
Staphylococcus
aureus
is
exposed
to
hypochlorous
acid
(HOCl)
and
hypothiocyanous
(HOSCN),
which
are
produced
by
the
neutrophil
myeloperoxidase
as
potent
antimicrobial
killing
agents.
In
this
work,
we
applied
RNAseq
transcriptomics,
Brx-roGFP2
biosensor
measurements,
phenotype
analyses
investigate
stress
responses
defense
mechanisms
of
S.
COL
toward
HOSCN
stress.
Based
on
transcriptome
profile,
exerts
strong
thiol-specific
oxidative,
electrophile,
metal
well
protein
damage
in
,
indicated
induction
HypR,
TetR1,
PerR,
QsrR,
MhqR,
CstR,
CsoR,
CzrA,
AgrA,
HrcA,
CtsR
regulons.
Phenotype
various
mutants
HOSCN-responsive
genes
revealed
that
reductase
MerA
conferred
highest
resistance
COL,
whereas
QsrR
MhqR
electrophile
regulons
do
not
contribute
protection.
measurements
bacillithiol
(BSH)-specific
Western
blot
a
oxidative
shift
redox
potential
(
E
BSH
)
increased
S
-bacillithiolations
indicating
oxidized
disulfide
(BSSB)
under
While
Δ
merA
mutant
was
delayed
recovery
reduced
overproduction
hypR
enabled
faster
due
efficient
detoxification.
Moreover,
both
were
shown
growth
assays.
summary,
provokes
response,
an
-bacillithiolation
.
IMPORTANCE
colonizes
skin
airways
but
can
also
lead
life-threatening
systemic
chronic
infections.
colonization
phagocytosis
immune
cells,
encounters
thiol-reactive
oxidant
HOSCN.
The
understanding
adaptation
important
identify
novel
drug
targets
combat
multi-resistant
isolates.
As
mechanism,
uses
flavin
MerA,
functions
protects
against
homologs
have
conserved
detoxification
other
bacteria,
including
intestinal
respiratory
pathogens.
studied
comprehensive
mode
action
its
effect
reversible
discover
new
oxidant.
These
findings
provide
leads
for
future
design
fight
pathogen
at
sites
Язык: Английский
The role of metals in hypothiocyanite resistance inEscherichia coli
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 8, 2024
ABSTRACT
The
innate
immune
system
employs
a
variety
of
antimicrobial
oxidants
to
control
and
kill
host-associated
bacteria.
Hypothiocyanite/hypothiocyanous
acid
(
-
OSCN/HOSCN)
is
one
such
oxidant
that
synthesized
by
lactoperoxidase,
myeloperoxidase,
eosinophil
peroxidase
at
sites
throughout
the
human
body.
HOSCN
has
potent
antibacterial
activity
while
being
largely
non-toxic
towards
cells.
molecular
mechanisms
which
bacteria
sense
defend
themselves
against
have
only
recently
begun
be
elaborated,
notably
discovery
bacterial
reductase
(RclA),
an
HOSCN-degrading
enzyme
widely
conserved
among
live
on
epithelial
surfaces.
In
this
paper,
I
show
Ni
2+
sensitizes
Escherichia
coli
inhibiting
glutathione
reductase,
inorganic
polyphosphate
protects
E.
effect,
probably
chelating
ions.
also
found
RclA
very
sensitive
inhibition
Cu
Zn
,
metals
are
accumulated
high
levels
cells,
that,
surprisingly,
thioredoxin
not
involved
in
stress
resistance
.
These
results
advance
our
understanding
contribution
different
oxidative
response
redox
buffering
pathways
illustrate
important
interactions
between
metal
ions
enzymes
use
stress.
IMPORTANCE
Hypothiocyanite
(HOSCN)
produced
system.
understood.
paper
significant
because
they
buffer
critical
components
response,
working
mechanism
distinct
from
HOSCN-specific
defenses
provided
RclA,
RclB,
RclC
proteins,
(including
nickel,
copper,
zinc)
may
impact
ability
resist
specific
defensive
e.g.
or
RclA).
Язык: Английский
The therapeutic potential of thiocyanate and hypothiocyanous acid against pulmonary infections
Free Radical Biology and Medicine,
Год журнала:
2024,
Номер
219, С. 104 - 111
Опубликована: Апрель 11, 2024
Язык: Английский
The role of metals in hypothiocyanite resistance in Escherichia coli
Journal of Bacteriology,
Год журнала:
2024,
Номер
206(8)
Опубликована: Авг. 22, 2024
ABSTRACT
The
innate
immune
system
employs
a
variety
of
antimicrobial
oxidants
to
control
and
kill
host-associated
bacteria.
Hypothiocyanite/hypothiocyanous
acid
(
−
OSCN/HOSCN)
is
one
such
oxidant
that
synthesized
by
lactoperoxidase,
myeloperoxidase,
eosinophil
peroxidase
at
sites
throughout
the
human
body.
HOSCN
has
potent
antibacterial
activity
while
being
largely
non-toxic
toward
cells.
molecular
mechanisms
which
bacteria
sense
defend
themselves
against
have
only
recently
begun
be
elaborated,
notably
discovery
bacterial
reductase
(RclA),
an
HOSCN-degrading
enzyme
widely
conserved
among
live
on
epithelial
surfaces.
In
this
paper,
I
show
Ni
2+
sensitizes
Escherichia
coli
inhibiting
glutathione
inorganic
polyphosphate
protects
E.
effect,
probably
chelating
ions.
also
found
RclA
very
sensitive
inhibition
Cu
Zn
,
metals
are
accumulated
high
levels
cells,
that,
surprisingly,
thioredoxin
not
involved
in
stress
resistance
.
These
results
advance
our
understanding
contribution
different
oxidative
responses
redox
buffering
pathways
illustrate
important
interactions
between
metal
ions
enzymes
use
stress.
IMPORTANCE
Hypothiocyanite
(HOSCN)
produced
system.
understood.
paper
significant
because
they
low
weight
thiol
critical
components
response,
working
mechanism
distinct
from
HOSCN-specific
defenses
provided
RclA,
RclB,
RclC
proteins
(including
nickel,
copper,
zinc)
may
impact
ability
resist
specific
defensive
(e.g.,
or
RclA).
Язык: Английский
Xanthine oxidase-lactoperoxidase system: Dose-dependent antibacterial effects and global gene expression changes in infant oral microbiota
Food Research International,
Год журнала:
2024,
Номер
201, С. 115596 - 115596
Опубликована: Дек. 30, 2024
Язык: Английский
Expression of RcrB confers resistance to hypochlorous acid in uropathogenicEscherichia coli
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Июнь 1, 2023
To
eradicate
bacterial
pathogens,
neutrophils
are
recruited
to
the
sites
of
infection,
where
they
engulf
and
kill
microbes
through
production
reactive
oxygen
chlorine
species
(ROS/RCS).
The
most
prominent
RCS
is
antimicrobial
oxidant
hypochlorous
acid
(HOCl),
which
rapidly
reacts
with
various
amino
acids
side
chains,
including
those
containing
sulfur
primary/tertiary
amines,
causing
significant
macromolecular
damage.
Pathogens
like
uropathogenic
Язык: Английский
Periodontal disease etiology and role of oxidative stress
Al-Kufa University Journal for Biology,
Год журнала:
2023,
Номер
15(3), С. 137 - 140
Опубликована: Дек. 31, 2023
The
term
"periodontal
disease"
is
used
to
describe
an
array
of
inflammatory
conditions
that
can
affect
the
periodontium.
In
response
challenge
given
by
bacteria,
gingival
and
periodontal
tissues
undergo
a
complicated
chain
events
involving
communication
It
between
membranes
vital
nature
located
under
gums
person's
immune
reaction.
When
there
imbalance
oxidants
antioxidants
in
body,
this
known
as
oxidative
stress.
Antioxidants
are
compounds
may
either
seek
out
destroy
or
prevent
them
from
forming
first
place.
Some
produced
by-products
normal
cellular
metabolism
include
reactive
oxygen
species
nitrogen
species.
These
byproducts
species,
respectively.
presence
(ROS)
has
been
shown
be
associated
with
disease
research.
Язык: Английский