Glutamic acid reshapes the plant microbiota to protect plants against pathogens

Microbiome, Год журнала: 2021, Номер 9(1)

Опубликована: Дек. 20, 2021

Plants in nature interact with other species, among which are mutualistic microorganisms that affect plant health. The co-existence of microbial symbionts the host contributes to fitness a natural context. In turn, composition microbiota responds environment and state host, raising possibility it can be engineered benefit plant. However, technology for engineering structure microbiome is not yet available.The loss diversity reduction population density Streptomyces globisporus SP6C4, core microbe, was observed coincident aging strawberry plants. Here, we show glutamic acid reshapes community enriches populations Streptomyces, functional microbe anthosphere. Similarly, tomato rhizosphere, treatment increased sizes as well those Bacillaceae Burkholderiaceae. At same time, diseases caused by species Botrytis Fusarium were significantly reduced both habitats. We suggest directly modulates community.Much known about plant-associated communities, but less understood how complexity controlled. Our results demonstrate intrinsic level planta associated microbiota, modulated an external supply biostimulant. Video Abstract.

Язык: Английский

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Biocontrol Ability of the Bacillus amyloliquefaciens Group, B. amyloliquefaciens, B. velezensis, B. nakamurai, and B. siamensis, for the Management of Fungal Postharvest Diseases: A Review

Journal of Agricultural and Food Chemistry, Год журнала: 2022, Номер 70(22), С. 6591 - 6616

Опубликована: Май 23, 2022

The Bacillus amyloliquefaciens group, composed of B. amyloliquefaciens, velezensis, nakamurai, and siamensis, has recently emerged as an interesting source biocontrol agents for the management pathogenic fungi. In this review, all reports regarding ability these species to control postharvest fungal diseases have been covered first time. showed various antifungal mechanisms, including production lipopeptides volatile organic compounds, competition nutrients, induction disease resistance. Most discussed their use fruit diseases. Several strains were studied in combination with additives, improving inhibitory efficacies. addition, a few commercialized. Overall, studies that are suitable environmentally friendly alternative However, there still crucial knowledge gaps improve efficacy host range.

Язык: Английский

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Evaluation of chitosan coatings enriched with turmeric and green tea extracts on postharvest preservation of strawberries

LWT, Год журнала: 2022, Номер 163, С. 113551 - 113551

Опубликована: Май 16, 2022

Strawberries are a widely consumed fruit that contains variety of antioxidant metabolites. However, postharvest strawberries highly perishable and susceptible to fungal pathogens. In this study, chitosan-based coating containing turmeric (TU) green tea (GT) extracts was developed evaluated for the preservation strawberries. Both TU GT contained high phenolic contents (>5.8 g kg−1 dry weight), but only extract showed strong antifungal activity. SEM observations revealed concentrations (3–5% v/v) caused formation agglomerates within films, reduced elongation at break increased tensile strength. The treatment with chitosan inhibited proliferation Botrytis cinerea during 7 d storage 20 °C, whereas extended properties from 4 8 °C. Sensory analysis indicated coatings did not significantly affect flavor taste. Overall, used first time food packaging purposes proved be great complement extend shelf-life

Язык: Английский

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Chemical composition and antifungal activity of essential oils and their combinations against Botrytis cinerea in strawberries

Journal of Food Measurement & Characterization, Год журнала: 2021, Номер 15(2), С. 1815 - 1825

Опубликована: Янв. 3, 2021

Язык: Английский

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Comparative performance of bio-based coatings formulated with cellulose, chitin, and chitosan nanomaterials suitable for fruit preservation

Carbohydrate Polymers, Год журнала: 2021, Номер 259, С. 117764 - 117764

Опубликована: Фев. 10, 2021

Язык: Английский

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BioClay™ prolongs RNA interference‐mediated crop protection against Botrytis cinerea

Journal of Integrative Plant Biology, Год журнала: 2022, Номер 64(11), С. 2187 - 2198

Опубликована: Авг. 30, 2022

One of the most promising tools for control fungal plant diseases is spray-induced gene silencing (SIGS). In SIGS, small interfering RNA (siRNA) or double-stranded (dsRNA) targeting essential virulence-related pathogen genes are exogenously applied to plants and postharvest products trigger interference (RNAi) targeted genes, inhibiting growth disease. However, SIGS limited by unstable nature under environmental conditions. The use layered double hydroxide clay particles as carriers deliver biologically active dsRNA, a formulation termed BioClay™, can enhance durability on plants, prolonging its activity against pathogens. Here, we demonstrate that dsRNA delivered BioClay prolong protection Botrytis cinerea, major pathogen, tomato leaves fruit mature chickpea plants. increased window from 1 3 weeks 5 10 days fruits, when compared with naked dsRNA. flowering provided prolonged up 4 weeks, covering critical period poding, whereas protection. This research represents step forward adoption an eco-friendly alternative traditional fungicides.

Язык: Английский

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Development of a single-cell atlas for woodland strawberry (Fragaria vesca) leaves during early Botrytis cinerea infection using single-cell RNA-seq

Horticulture Research, Год журнала: 2022, Номер 9

Опубликована: Янв. 1, 2022

Abstract Pathogen invasion leads to fast, local-to-systemic signal transduction that initiates plant defense responses. Despite tremendous progress in past decades, aspects of this process remain unknown, such as which cell types respond first and how signals are transferred among types. Here, we used single-cell RNA-seq >50 000 single cells document the gene expression landscape leaves woodland strawberry during infection by Botrytis cinerea identify major We constructed a atlas characterized distinct patterns hydathode, epidermal, mesophyll incubation period B. infection. Pseudotime trajectory analysis revealed transition from normal functioning response epidermal upon Genes related disease resistance showed different types: resistance-related genes encoding transcription factors were highly expressed individual interacted trigger systemic immunity cinerea. This is report transcriptional pathogenic process; it provides new insights into holistic dynamics host–pathogen interactions can guide identification formulation strategies for resistant cultivar development.

Язык: Английский

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Monitoring and prediction of sensory shelf‐life in strawberry with ultraviolet‐visible‐near‐infrared (UV‐VIS‐NIR) spectroscopy

Applied Food Research, Год журнала: 2022, Номер 2(2), С. 100123 - 100123

Опубликована: Май 14, 2022

The suitability of UV-VIS-NIR reflectance for predicting the sensory shelf-life (SSL) and number days under refrigerated storage (DS) strawberries was examined. performance different classification methods compared. Partial least squares regression (PLSR) models were calibrated evaluated resulting in similar with NIR (R2 = 0.870), UV-VIS 0.874), 0.877) datasets evaluation sets. estimated from visual scores a panel. Based on shelf life each strawberry their storage, remaining till rejection (DTR) calculated. PLSR trained to predict DTR up R2 0.712 influence examined simple models. residuals improve prediction DTR. built predictor dataset containing PCA denoised Savistzky-Golay (SG) second derivate transformed data extended multiplicative signal corrected (EMSC) first SG data. achieved 0.818 Repeated feature selection carried out using Uninformative Variable Elimination (UVE-PLS) shelf-life. wavebands selected more frequently by DS recorded, visualized, interpreted.

Язык: Английский

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Control of postharvest diseases in berries through edible coatings and bacterial probiotics

Scientia Horticulturae, Год журнала: 2022, Номер 304, С. 111326 - 111326

Опубликована: Июль 8, 2022

The world's population is growing, which requires more resources, including food. Some necessary foods, such as berries, are very perishable fresh products that suffer contamination by pathogens, generating great economic losses. Various physical and chemical strategies have been used to mitigate these losses over the years, use of pesticides. However, negative impact on environment human health has aroused interest in development other control methods. Biocontrol one innovative strategies, various biological agents can be used, bacteria probiotics. Probiotics act antagonists fungal pathogens competition for space nutrients, production secondary metabolites, volatile organic compounds (VOCs), lytic enzymes, activation plant defenses. On hand, there materials protection against seen, edible coatings, since they components, chitosan, with antimicrobial properties. In addition, probiotics conjunction elements resulting from a new strategy post-harvest diseases. This review compiles studies and/or coatings method reducing diseases, specifically, berries.

Язык: Английский

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Pathogenic Drug Resistant Fungi: A Review of Mitigation Strategies

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(2), С. 1584 - 1584

Опубликована: Янв. 13, 2023

Fungal pathogens cause significant human morbidity and mortality globally, where there is a propensity to infect vulnerable people such as the immunocompromised ones. There increasing evidence of resistance antifungal drugs, which has implications for cutaneous, invasive bloodstream infections. The World Health Organization (WHO) published priority list fungal in October 2022, thus, highlighting that crisis point been reached pressing need address solutions. This review provides timely insight into challenges on topic drug along with discussing effectiveness established disease mitigation modalities approaches. also elucidate cellular molecular mechanisms inform effective decontamination approaches are medical device processing sterilization, but presence pathogenic fungi recalcitrant biofilms can lead challenges, particularly during cleaning. Future design ideas implantable reusable devices should consider materials appropriates disinfection, it relevant, sterilization. Preventing growth mycotoxin-producing foods through use appropriate end-to-end processes advisable, mycotoxins challenging eliminate once they have formed.

Язык: Английский

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