Harnessing the genomic diversity of Pseudomonas strains against lettuce bacterial pathogens

Frontiers in Microbiology, Год журнала: 2022, Номер 13

Опубликована: Дек. 22, 2022

Lettuce is a major vegetable crop worldwide that affected by numerous bacterial pathogens, including Xanthomonas hortorum pv. vitians, Pseudomonas cichorii, and Pectobacterium carotovorum. Control methods are scarce not always effective. To develop new sustainable approaches to contain these we screened more than 1,200 plant-associated strains retrieved from agricultural soils for their in vitro antagonistic capabilities against the three pathogens under study. Thirty-five significantly inhibited some or all pathogens. Their genomes were fully sequenced annotated. These belong P. fluorescens putida phylogenomic groups distributed at least 27 species, 15 validly described species. They harbor genes clusters of known be involved plant-bacteria interactions, microbial competition, biocontrol. Strains group displayed on average better inhibition abilities group. carry biosynthetic mostly absent latter production secondary metabolites such as 7-hydroxytropolone, putisolvins, pyochelin, xantholysin-like pseudomonine-like compounds. The presence biosynthesis type VI secretion systems, tailocins, hydrogen cyanide also positively correlated with strains' overall observed results show promise development biocontrol products lettuce provide insights potential mechanisms involved, contribute public genome databases, quality sequences poorly represented

Язык: Английский

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Differentiation of the Xanthomonas hortorum – Xanthomonas hydrangeae Species Complex Using Sensitive and Selective LAMP Assays

Frontiers in Agronomy, Год журнала: 2022, Номер 4

Опубликована: Июнь 1, 2022

The seven pathovars of Xanthomonas hortorum and hydrangeae , referred to as the X. – species complex, cause disease on a multitude plants, including crops, ornamental wild plants. Cross-pathogenicity was proven for some strains within this complex. It is thus important have highly specific fast diagnostics methods members A comparative genomic analysis conducted representative complex identify singletons use targets assays. Seven loop-mediated isothermal amplification (LAMP) assays were developed detection six clades in addition one assay entire Primer sets tested set 62 reference strains. primer amplified their respective 15 minutes. Based set, all had sensitivity, specificity, efficiency 100%. used validation 60 According LAMP results, out strains, 39 assigned 9 but yet undefined 12 previously misclassified since DNA did not yield with any genome-based are promising diagnostic tools various assigning new historical isolates

Язык: Английский

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Bacterial leaf streak: Inoculation methodology determination

Tropical Plant Pathology, Год журнала: 2023, Номер 48(6), С. 720 - 727

Опубликована: Сен. 12, 2023

Язык: Английский

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Characterization of Emerging Xanthomonas campestris Isolates on the Nonnative Weed Garlic Mustard (Alliaria petiolata)

Plant Disease, Год журнала: 2024, Номер 108(6), С. 1418 - 1424

Опубликована: Янв. 11, 2024

Nonnative plant infestations provide unique opportunities to investigate pathogen emergence with evolutionarily recent introduction events. The widespread distribution of invasive plants and their proximity genetically related crops highlights the risks nonnative acting as ancillary hosts fostering microbial recombination selection. Garlic mustard (

Язык: Английский

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The alternative host with the most: Understanding the ecology of Xanthomonas hortorum pv. carotae on non-carrot crops in central Oregon

Plant Disease, Год журнала: 2024, Номер 108(6), С. 1755 - 1761

Опубликована: Янв. 12, 2024

Bacterial blight of carrot, caused by Xanthomonas hortorum pv. carotae (Xhc), is an economically important disease in carrot (Daucus carota subsp. sativus) seed production. The objectives this study were to determine if Xhc was present on noncarrot crops grown central Oregon and, detected, evaluate its ability colonize alternative hosts. Surveys three fields and adjacent rye (Secale cereale), alfalfa (Medicago sativa), parsley root (Petroselinum crispum var. tuberosum), Kentucky bluegrass (Poa pratensis) demonstrated that crops. Greenhouse experiments conducted the cultivated region. Carrot, alfalfa, curly crispum), bluegrass, mint (Mentha × piperita), root, roughstalk trivialis), wheat (Triticum aestivum) plants spray-inoculated with destructively sampled at 1, 7, 14, 28 or 25 days post-inoculation. populations quantified using viability quantitative PCR dilution plating. A significant (P ≤ 0.03) effect crop observed both experiments. While hosted most final timepoint, other supported epiphytic including bluegrasses. Mint, least Xhc. symptoms carrots but not This suggests have potential be asymptomatically colonized may serve as reservoirs pathogen production systems.

Язык: Английский

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Time of arrival during disease progression and humidity additively influenceSalmonella entericacolonization of lettuce

Опубликована: Фев. 17, 2024

ABSTRACT The interplay between plant host, phytopathogenic bacteria, and enteric human pathogens in the phyllosphere have consequences for health. Salmonella enterica has been known to take advantage of phytobacterial infection increase its success on plants, but there is little knowledge additional factors that may influence relationship pathogen disease. In this study, we investigated role humidity extent disease progression S. colonization plants. We found high was necessary replication diseased lettuce, not required ingress into UV-protected apoplast. Additionally, Xanthomonas hortorum pv. vitians (hereafter, X. ) - infected lettuce host be a relatively hostile environment when it arrived prior development watersoaking or following necrosis onset, supporting existence an ideal window during progress maximizes survival . vitro growth studies sucrose media suggest allow benefit from cross-feeding infection. Overall, study emphasizes as driver phyllosphere, demonstrates how time arrival can enterica’s fate apoplast, highlights potential transform apoplast growth-promoting bacterial colonizers. Importance: Bacterial leaf spot caused by common threat leafy green production. global impact phytopathogens, including , likely with climate change. even under scenario where increased did enhance disease, had substantial effect facilitating Xanthomonas- High climates directly contribute crop fields indirectly affect via changes brought phytopathogen

Язык: Английский

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Eliminating the Pathogen Xanthomonas hortorum pv. carotae from Carrot Seeds Using Different Types of Nanoparticles

Agriculture, Год журнала: 2024, Номер 14(3), С. 498 - 498

Опубликована: Март 19, 2024

There exists a wide range of plant pathogens that are commonly referred to as seed-borne due their dominant mode spread. Treating seeds eliminate such is therefore very important in contemporary seed production. In the present study, eight types nanoparticles were evaluated for effectiveness against Xanthomonas hortorum pv. carotae, pathogen affects plants Apiaceae family. Initially, parameters considering inhibitory and bactericidal activity individual under vitro conditions. this way, three based on copper, silver, silver/selenium composite identified being most effective. Subsequently, ability carotae from artificially infected carrot was tested. This achieved through qPCR quantification 14-day-old plantlets developed inoculated with Xhc. Based obtained results, copper-based effective, resulting an approximately 10-fold decrease occurrence Xhc compared untreated control. Taking into account fact X. pathovars also attack other horticultural crops, presented results may have much wider scope than just seeds.

Язык: Английский

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Management of bacterial blight of carrots by phenolic compounds treatment

PLoS ONE, Год журнала: 2024, Номер 19(4), С. e0299105 - e0299105

Опубликована: Апрель 1, 2024

Bacterial blight is a serious disease of carrot production worldwide. Under favorable conditions, the causal organism Xanthomonas hortorum pv. carotae causes loss especially in seed because its seed-borne character. Unlike fungal diseases, treatment bacterial diseases limited and methods such as hot water or sodium hypochlorite (bleach) are mainly used by companies. Here, we compared efficacy treatment, with three phenolic compounds–carvacrol, thymol eugenol, to eliminate growth vitro subsequently vivo on seeds Xhc low, medium highly infested lots. The complete elimination from germinated plants was obtained only for low lot 1% carvacrol solutions concentrations 0.0196%– 0.313%. significant reduction presence achieved treatment. However, resulted germination percentage well. Considering infection effect plant vigor, 0.0196% solution suggested an alternative regarding additional costs related liquidation treated that has been proved be insufficient obtain disease-free plants.

Язык: Английский

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Molecular-Phylogenetic Characterization of Xanthomonas hortorum pv. pelargonii Strains Causing Leaf Spot of Geraniums in Iran

Plant Disease, Год журнала: 2024, Номер 108(9), С. 2701 - 2709

Опубликована: Апрель 10, 2024

Bacterial blight and leaf spot of geraniums is a destructive disease cultivated

Язык: Английский

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Early Stages of Bacterial Colonization Development After Xanthomonas campestris Seed Inoculation in Selected Brassica Species

Horticulturae, Год журнала: 2024, Номер 10(12), С. 1307 - 1307

Опубликована: Дек. 9, 2024

This study investigates Xanthomonas campestris pv. (Xcc) incidence rates in cabbage, kale, and kohlrabi, focusing on the roots stems over a 15-day period after sowing. Seeds were inoculated with Xcc, infection levels monitored using confocal microscopy combined fluorescence situ hybridization. Significant differences observed across 15 days scale from 0 to 5 (0–100% incidence). Kale exhibited highest mean (3.64), while cabbage kohlrabi showed greater variability (3.4–3.44). Stem infections more severe, kale showing (4.16), followed by (4.0), (3.68). In roots, increased until day 11, which significant decline was noted, whereas stem Xcc remained stable. peaked 9, fluctuations 13 15. Kohlrabi displayed moderate, stable root period. stems, increase drop Regression analysis revealed positive linear correlation between rate of observation roots. However, no trends all species, where high 7 onwards. Newly acquired data indicate that development depends type vegetable. bacterial occurrence parts does not always correspond intensity colonization section plants. Even at lower damage vascular bundles can be fatal. These findings offer valuable insights into dynamics, contributing improved disease management strategies for Brassica crops.

Язык: Английский

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