Опубликована: Янв. 1, 2024
Язык: Английский
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
1
Опубликована: Фев. 15, 2024
Ralstonia solanacearum is the pathogen responsible for solanacearum, which soil-borne, infests plant conduits to cause disease.They are not easy control and harmful.Therefore development of a controlled-release agent responsive pathogen's soil temperature moisture conditions could aid in its control. Corn kernel powder used as carrier Tetramycin biopesticide, with various compounds tested bonding.They were then made by extrusion moulding.Temperature wetness components, including Pentaerythritol Tetrastearate, Tetraoleate, Polyethylene Glycol Stearate, Monooleate,are compounded coated onto cores form an intermediate shell layer. Finally, Ethyl cellulose(EC)and Hydroxypropyl methyl cellulose(HPMC) dissolved sprayed outermost layer complete pellet's layer.After evaluation texture performance test,DSC analysis,TG test,temperature humidity response test, well pellet cross-section SEM observation, field effectiveness etc., preferred formulations screened.The results showed that Core formulation:Corn powder:Tetramycin aqueous:Xanthan gum mass ratio=13.5:23:2; Intermediate Shell layer:PETS: PETO: PEG400MO ratio=10:30:10; Outer layer: cellulose (EC):Hydroxypropyl (HPMC) ratio=5:1.Under high incidence i.e., sustained warmth (30-35℃) (30% content), formulated pills release rate 95.18% 96.24% over period 35 days. Moreover, they stepwise cyclic process under alternating warm humid-dry cold conditions.In tests on tobacco, relative preventive efficacy 54.74% was achieved 106 days post-transplanting. Soil bacterial community structure analysis revealed significant decrease (2.28-2.84 times) abundance Solanaceae Raelia treatment compared Additionally, fungal highter consistency initially across depths, diversity differences between layers reestablished later (106 days), while predominant categories bacteria fungi remained unchanged.In this study, we successfully developed nucleoshell-type slow-controlled pill embedded Tetramycin. This effectively responded changes, resulting disease prevention.This holds promise improving drug utilization easing application challenges, offering broad prospects prevention agriculture.
Язык: Английский
Процитировано
4
Heliyon, Год журнала: 2025, Номер 11(2), С. e41903 - e41903
Опубликована: Янв. 1, 2025
Potato (Solanum tuberosum) is an important global food source, the growth of which can be severely impacted by Ralstonia solanacearum bacterial infection. Despite extensive research, molecular mechanisms potato resistance to this pathogen are imperfectly known. Huashu No. 12, a tetraploid genotype, highly resistant R. solanacearum. We inoculate 12 and Longshu 7 (highly susceptible solanacearum) with compare disease in these two varieties. has significantly higher infection than 7, increased lignin content, abundance callose strong autofluorescence phloem sieve tube. Enzymes (e.g., superoxide dismutase, catalase, peroxidase, phenylalanine ammonia-lyase, polyphenol oxidase) contribute 12. Transcriptome sequencing reveals 659 differentially expressed genes between varieties, ethylene responsive factor family containing most genes. Gene ontology KEGG analyses provided further insights into genetic basis underlying plant defense against disease. By demonstrating importance enzymes differential gene expression infection, breeding disease-resistant becomes increasingly feasible.
Язык: Английский
Процитировано
0
Plant Stress, Год журнала: 2025, Номер unknown, С. 100804 - 100804
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Cell Reports, Год журнала: 2024, Номер 43(8), С. 114596 - 114596
Опубликована: Авг. 1, 2024
The Ralstonia solanacearum species complex causes bacterial wilt in a variety of crops. Tomato cultivar Hawaii 7996 is widely used resistance resource; however, the evaded by virulent strains, with underlying mechanisms still unknown. Here, we report that phylotype Ⅱ strain ES5-1 can overcome resistance. RipV2, type Ⅲ effector specific to vital overcoming tomato which encodes an E3 ubiquitin ligase, suppresses immune responses and Toll/interleukin-1 receptor/resistance nucleotide-binding/leucine-rich repeat (NLR) (TNL)-mediated cell death. helper NLR N requirement gene 1 (NRG1), enhanced disease susceptibility (EDS1), senescence-associated 101b (SAG101b) are identified as RipV2 target proteins. essential for virulence but not SlNRG1-silenced tomato, demonstrating SlNRG1 be target. Our results dissect disrupting immunity highlight importance converged components conferring
Язык: Английский
Процитировано
2
Agronomy, Год журнала: 2024, Номер 14(6), С. 1199 - 1199
Опубликована: Июнь 1, 2024
Plant bacterial wilt is caused by Ralstonia solanacearum, a soilborne pathogen that infects plant conduits, leading to disease. It extremely difficult cure plants infected with solanacearum; however, bactericide-loaded beads hot-/wet-responsive properties may be able release biocide in line the increase hot-/wet-associated activity of effectively killing pathogenic cells and providing high levels protection. A biopesticide, Tetramycin, was embedded corn kernel powder (CKP)-based cores. An oil-phase mixture sprayed onto core surface form intermediate shell (IMS). Subsequently, layer ethyl cellulose (EC) hydroxypropyl methyl (HPMC) coated IMS create single wet-responsive outer (OTS). The ratios components cores, including (CKP), xanthan gum (XG), were optimized, as well those IMS, pentaerythrityl tetrastearate (PETS), tetraoleate (PETO), polyethylene glycol stearate (PEG400MS), monooleate (PEG400MO), (OTS), (HPMC). texture performance analysis, differential scanning calorimetry (DSC) thermogravimetric analysis (TGA), temperature humidity response tests, electron microscope (SEM) observations, field effectiveness test conducted characterize Tetramycin-loaded beads. results indicated optimal formula for bead cores comprised mass ratio CKP/Tetramycin solution/XG = 13.5:23:2. preferred PETS/PETO/PEG400MO 10:30:10, applicable OTS consisted EC/HPMC 5:1. In soil 30–35 °C 30%, period beads, cumulative rate over 95%, could last up 35 days. Furthermore, exhibited gradual multi-cyclic process under alternating hot/wet dry/cold environments. relative preventive efficacy 54.74% on tobacco revealed at field-testing scale. significant reduction abundance solanacearum also observed treatment early fungal community structure higher consistency compared control. However, later stage, diversity differences between layers restored. conclusion, respond fluctuations developed, resulting enhanced disease prevention offering broad prospects control agricultural settings.
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown
Опубликована: Окт. 16, 2024
Abstract Members of the Ralstonia solanacearum species complex are soil-borne pathogens important crop plants. Strain-level host specialisation is considered typical, yet outbreak monitoring in Martinique revealed emergence a new lineage with dramatically expanded range. We show was caused by parallel expansion lineages from two different species. One Asian origin retains signature specialisation, while emergent broad range originated Americas. The presence similar integrative and conjugative elements both suggests they exchanging genetic material. acquired genes encoding novel catalytic metabolic functions, but shows little variation among virulence specificity determinants. event not evolution strategy targeting immunity, more likely due to acquisition substrate utilisation pathways enhancing pathogen fitness soil or plant tissues.
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0